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2 protocols using phenazine methosulfate pms

1

Sodium Arsenite and Tetrandrine Cytotoxicity Assay

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Sodium arsenite (NaAsO2, AsIII) (>99% purity) and tetrandrine (99.2% purity) were purchased from Tri Chemical Laboratories (Yamanashi, Japan) and National Institutes for Food and Drug Control (Beijing, China), respectively. Fetal bovine serum (FBS) was purchased from Nichirei Biosciences (Tokyo, Japan). Dulbecco’s modified Eagle’s medium (DMEM), RPMI-1640 medium, phenazine methosulfate (PMS), and dimethyl sulfoxide (DMSO) were obtained from Wako Pure Chemical Industries (Osaka, Japan). Propidium iodide (PI), proteinase K, ribonuclease A (RNaseA), 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)3.2-5-[(phenylamino)carbony]-2H-tetrazolium hydroxide (XTT), and tamoxifen were purchased from Sigma-Aldrich (St. Louis, MO, USA). MAPK inhibitors and their negative controls (JNK inhibitor SP600125 and its negative control SP600125NC; p38 MAPK inhibitor SB203580 and its negative control SB202474; ERK inhibitor PD98059) were purchased from Calbiochem (La Jolla, CA, USA). Autophagy inhibitors, 3-methyladenine (3-MA) and wortmannin, were purchased from Wako Pure Chemical Industries and Calbiochem, respectively. Can Get Signal® Immunoreaction Enhancer Solution was purchased from Toyobo Co., Ltd. (Osaka, Japan).
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2

Synergistic Anti-Cancer Effects of Sodium Arsenite and Tetrandrine

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Sodium arsenite (NaAsO2, AsIII) and tetrandrine (Tetra) were purchased from Tri Chemical Laboratories (Yamanashi, Japan) and National Institutes for Food and Drug Control (Beijing, China), respectively. Fetal bovine serum (FBS) was purchased from Nichirei Biosciences (Tokyo, Japan). Dulbecco’s modified Eagle’s medium (DMEM), phenazine methosulfate (PMS) and dimethyl sulfoxide (DMSO) were obtained from Wako Pure Chemical Industries (Osaka, Japan). Wortmannin, a potent autophagy inhibitor, propidium iodide (PI), ribonuclease A (RNaseA) and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tetrandrine hydrochloride injection and arsenious acid and sodium chloride injection were obtained from Jiangxi Yintao Pharmaceutical Co., Ltd. (Jiangxi, China) and Heilongjiang Harbin Pharmaceutical Co., Ltd. (Heilongjiang, China), respectively for the in vivo study. Can Get Signal® Immunoreaction Enhancer Solution were purchased from Toyobo Co., Ltd. (Osaka, Japan).
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