4,4′-Azobis(4-cyanovaleric acid) (ACVA), METAC, and APMAA were purchased from Sigma-Aldrich (St Louis, MO, USA) and were used without purification. Acetic acid, tetramethylammonium hydroxide pentahydrate, and N-hydroxysuccinimide were purchased from FUJIFILM Wako Pure Chemical (Chuo-Ku, Osaka, Japan), and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (WSCD-HCl) was purchased from Peptide Institute, Inc. (Ibaraki, Osaka, Japan). HpD was purchased from MedChem Express (San Diego, CA, USA).
N hydroxysuccinimide
Manufactured by Fujifilm
Sourced in Japan
N-hydroxysuccinimide is a chemical compound that is commonly used as a reactive group in organic synthesis. It is a versatile reagent that can be used to activate carboxylic acids for coupling with primary amines, forming stable amide bonds. This process is often utilized in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride wscd hcl, by Peptide Institute (2 mentions)
Ll 37 fragment 17 29, by Funakoshi (2 mentions)
Ckrivkrivkkwlr, by Toray (2 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride, by Fujifilm (2 mentions)
Sodium azide, by Fujifilm (2 mentions)
Dioxane, by Fujifilm (2 mentions)
3 aminopropyltrimethoxysilane, by Tokyo Chemical Industry (2 mentions)
Acetic acid, by Fujifilm (1 mentions)
Hyaluronic acid, by Tokyo Chemical Industry (1 mentions)
Prednisolone, by Fujifilm (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride, by Dojindo Laboratories (1 mentions)
Sudan 3, by Fujifilm (1 mentions)
Methanol, by Fujifilm (1 mentions)
Rpmi 1640 medium without phenol red, by Thermo Fisher Scientific (1 mentions)
4 4 azobis 4 cyanovaleric acid acva, by Merck Group (1 mentions)
Ripa buffer, by Nacalai Tesque (1 mentions)
Live dead cell staining kit, by Dojindo Laboratories (1 mentions)
2 7 dichlorodihydrofluorescein diacetate dcfh da, by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Ez link nhs peg4 biotin, by Thermo Fisher Scientific (1 mentions)
17 protocols using n hydroxysuccinimide
1
Synthesis of Polymeric Photosensitizers
2
Peptide-Polymer Nanoparticle Synthesis
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The LL-37 fragment (17–29) was purchased from Funakoshi Co., Ltd. (Tokyo, Japan). The mutant peptide (CKRIVKRIVKKWLR) was synthesised by Toray Research Center (Tokyo, Japan). Poly (d, l-lactic-co-glycolic acid) (PLGA7510, MW: 10,000), 3-(2-pyridyldithio) propionylhydrazide, N, N-dicyclohexylcarbodiimide, and N-hydroxysuccinimide were purchased from Wako Pure Chemical Industries (Osaka, Japan). Candida albicans (JCM 1542) was purchased from the Japan Collection of Microorganisms in the Riken BioResource Research Center (Ibaragi, Japan).
3
Synthesis and Characterization of Glycoprotein Adjuvants
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Prednisolone, carbonyl-diimidazole (CDI), dimethyl-aminopyridine (DMAP), N-hydroxysuccinimide (NHS), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (WSC), and sodium alginate (AL-Na; low viscosity grade (viscosity of 1% aqueous solution at 25 °C = 32.7 ± 0.4 mPa·s (n = 3)), cat no. 154725, lot no. QR13046) were purchased from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan). N-Trityl-glycine (Tr-G) was obtained from Sigma-Aldrich Co., LLC. (St. Louis, MO). Hyaluronic acid (viscosity of 1% aqueous solution at 25 °C = 1.2 ± 0.1 mPa·s (n = 3), product code H0595, lot no. 8FUKM-CI) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Non-viable and desiccated Mycobacterium tuberculosis H37 Ra was obtained from Becton, Dickinson and Company (Franklin Lakes, NJ) and used in the preparation of adjuvants. All other chemicals were of reagent grade.
4
Dopamine Conjugation Using Carbodiimide
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3,4-Dihydroxyphenethylamine hydrochloride (dopamine) and N-hydroxysuccinimide were purchased from Wako Pure Chemical Industries (Osaka, Japan). 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (water-soluble carbodiimide) was obtained from Dojindo Molecular Technologies, Inc., (Kumamoto, Japan).
5
Antimicrobial Peptide Functionalization Protocol
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The LL-37 fragment (17–29) was purchased from Funakoshi Co., Ltd. (Tokyo, Japan). The mutant peptide (CKRIVKRIVKKWLR) was synthesized by the Toray Research Center (Tokyo, Japan). Poly (d ,l -lactic-co-glycolic acid) (PLGA7510, MW: 10,000), 3-(2-pyridyldithio) propionylhydrazide (PDPH), N,N′-dicyclohexylcarbodiimide, N-hydroxysuccinimide, and Sudan III were purchased from Wako Pure Chemical Industries (Osaka, Japan).
Three microbial strains were purchased from the Biological Resource Center, National Institute of Technology and Evaluation (NBRC) (Tokyo, Japan) and Japan Collection of Microorganisms in Riken BioResource Research Center (Ibaragi, Japan). The strains used in this study were S. aureus (NRBC 12732), E. coli (NRBC 3972), and C. albicans (JCM 1542).
Three microbial strains were purchased from the Biological Resource Center, National Institute of Technology and Evaluation (NBRC) (Tokyo, Japan) and Japan Collection of Microorganisms in Riken BioResource Research Center (Ibaragi, Japan). The strains used in this study were S. aureus (NRBC 12732), E. coli (NRBC 3972), and C. albicans (JCM 1542).
6
Hematoporphyrin Dihydrochloride Cellular Assays
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Hematoporphyrin dihydrochloride (HP) was purchased from MedChem Express (San Diego, CA, USA). RPMI-1640 medium with L-glutamine and phenol red, methanol, and N-hydroxysuccinimide were purchased from FUJIFILM Wako Pure Chemical (Osaka, Japan). RPMI 1640 medium without phenol red was purchased from Thermo Fisher Scientific (San Jose, CA, USA). RIPA buffer was purchased from Nacalai Tesque (Kyoto, Japan). 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (WSCD-HCl) was purchased from Peptide Institute, Inc. (Osaka, Japan). Trypsin-ethylenediaminetetraacetic acid (EDTA), [2-(methacryloyloxy)ethyl]trimethylammonium chloride (METAC), 4,4′-azobis(4-cyanovaleric acid) (ACVA), and N-(3-aminopropyl)methacrylamide hydrochloride (APMAA) were purchased from Sigma-Aldrich (St Louis, MI, USA). 2,2,6,6-Tetramethyl-4-piperidinol (4-OH-TEMP) was purchased from the Tokyo Chemical Industry (Tokyo, Japan). Fetal bovine serum (FBS) was purchased from Gibco (Waltham, MA, USA). The LIVE/DEAD Cell Staining Kit was purchased from Dojindo (Kumamoto, Japan). The Apoptosis/Necrosis Assay Kit (ab176749) was purchased from Abcam (Cambridge, UK). 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
7
Biotin-Avidin Surface Functionalization
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NeutrAvidin
and EZ-Link NHS-PEG4-biotin were
purchased from Thermo Fisher Scientific (Waltham, MA). Biotinylated
DNA and fluorescent-tagged oligonucleotides were purchased from Eurofin
Genomics (Tokyo, Japan). Manicure was purchased from Shiseido (Tokyo,
Japan). 3-Aminopropyltriethoxysilane (APTES) was purchased from Sigma
Aldrich (St. Louis, MO). Boric acid was purchased from Nacalai Tesque
(Kyoto, Japan). 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
(HEPES) and ethylenediaminetetraacetic acid (EDTA) were purchased
from Dojindo (Kumamoto, Japan). A cover glass (18 mm × 18 mm,
no. 1S, 0.175 ± 0.015 mm thickness) and a slide glass (76 mm
× 26 mm, S-0314 Neo no. 2, 1.0–1.2 mm thickness) were
purchased from Matsunami Glass Industries (Osaka, Japan). 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide
(EDC) was purchased from Tokyo Chemical Industry (Tokyo, Japan). N-Hydroxysuccinimide (NHS) was purchased from Wako Pure
Chemical Industries (Osaka, Japan). All of the other reagents were
purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan),
unless otherwise specified, and used without further purification.
Type 1 ultrapure water (Milli-Q) was used in all of the experiments.
and EZ-Link NHS-PEG4-biotin were
purchased from Thermo Fisher Scientific (Waltham, MA). Biotinylated
DNA and fluorescent-tagged oligonucleotides were purchased from Eurofin
Genomics (Tokyo, Japan). Manicure was purchased from Shiseido (Tokyo,
Japan). 3-Aminopropyltriethoxysilane (APTES) was purchased from Sigma
Aldrich (St. Louis, MO). Boric acid was purchased from Nacalai Tesque
(Kyoto, Japan). 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
(HEPES) and ethylenediaminetetraacetic acid (EDTA) were purchased
from Dojindo (Kumamoto, Japan). A cover glass (18 mm × 18 mm,
no. 1S, 0.175 ± 0.015 mm thickness) and a slide glass (76 mm
× 26 mm, S-0314 Neo no. 2, 1.0–1.2 mm thickness) were
purchased from Matsunami Glass Industries (Osaka, Japan). 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide
(EDC) was purchased from Tokyo Chemical Industry (Tokyo, Japan). N-Hydroxysuccinimide (NHS) was purchased from Wako Pure
Chemical Industries (Osaka, Japan). All of the other reagents were
purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan),
unless otherwise specified, and used without further purification.
Type 1 ultrapure water (Milli-Q) was used in all of the experiments.
8
Chitosan-Based Biomaterial Synthesis
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Chitosan with 83% of degree of deacetylation was purchased from KIMICA Corporation (Tokyo, Japan). Sodium gluconate, N-hydroxysuccinimide (NHS), Poly (vinyl alcohol) (PVA) (degree of saponification 96%, degree of polymerization 1000), and lysozyme from egg white were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide monohydrochloride (EDC) was purchased from Peptide Institute, Inc. (Osaka, Japan).
9
Labelling Alginate Gel Cores with Rhodamine 123
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Alginate gel cores without E. coli were labelled with rhodamine 123 (Wako) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Tokyo Chemical Industry Co., Ltd., TCI, Tokyo, Japan) and N-hydroxysuccinimide (Wako)56 . AGMs containing rhodamine 123-labelled cores were heated at 65 °C for 5 min in the presence of 50 mM EDTA or 50 mM CaCl2. Residual cores were observed as mentioned above.
10
Synthesis of HA-TA Conjugate Biomaterial
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The HA-TA conjugate was synthesized according to previously published methods [12 (link), 18 (link)]. Briefly, TA (final concentration 12.5 mM; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was dissolved in 25 mM 50 kDa HA solution (Kewpie Corporation Fine Chemical Division, Tokyo, Japan), and 2.5 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (FUJIFILM Wako Pure Chemical Corporation) and 2.5 mM N-hydroxysuccinimide (FUJIFILM Wako Pure Chemical Corporation) were added to start the conjugation reaction. Addition of 0.1 M NaOH was used to maintain the pH at 4.7 throughout the reaction. After stirring overnight at room temperature, the pH of the solution was raised to 7.0, and the solution was subsequently placed in dialysis tubes with a molecular cutoff of 1 kDa. Dialysis was performed in the tubes against 100 mM solution of sodium chloride for 2 days, a distilled water and ethanol mixture (3:1) for 1 day, and distilled water for 1 day. HA-TA was finally obtained by lyophilizing the purified solution.
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