Loxl2

LOX enzyme
was extracted from pig skin by the method of Shackleton and Hulmes.^{51 (link)} LOXL2 and LOXL3 were purchased from R&D
Systems. LOX, LOXL2, and LOXL3 catalytic activity were determined
using the Promega ROS-Glo assay kit with cadaverine dihydrochloride
as substrate, BAPN as the reference inhibitor control, a preincubation
time of 20 min, 1 h, or 3 h, with nine dilutions from a top concentration
of 10 μM or 100 μM.

LOX enzyme
was extracted from pig skin by the method of Shackleton and Hulmes.^{24 (link)} LOX catalytic activity was determined using
a horseradish peroxidase (HRP)-coupled fluorescent assay previously
described,^{19 (link)} with cadaverine hydrochloride
as a substrate, BAPN as positive control, and a preincubation time
of 20 min with nine dilutions from a top concentration of 100 μM.
LOXL2 was purchased from R&D System. LOXL2 catalytic activity
was determined using the Promega ROS-Glo assay kit with cadaverine
hydrochloride as a substrate, BAPN as positive control, and a preincubation
time of 20 min at the same concentrations as above.