Anti alb

Protein expression was analyzed using Western blotting as previously described (Yu et al. 2019 (link); Gong, Yan, et al. 2019 (link)). For Western blot analysis, primary antibodies against IL-6 (mouse monoclonal antibody, ab9324) and nephrin (rabbit monoclonal antibody, ab216341) were purchased from Abcam (UK), Anti-TGF-β1 (mouse monoclonal antibody, sc-130348) was purchased from Santa Cruz (USA), anti-podocin (rabbit polyclonal antibody, TA351459) was purchased from Origene (USA), anti-caspase-3 (rabbit polyclonal antibody, 9662S) was purchased from CST (USA), and anti-ALB (rabbit polyclonal antibody, 16475-1-AP) was purchased from Proteintech (USA). Goat anti-mouse IgG-HRP (ZB2305) and goat anti-rabbit (ZB2301) IgG-HRP secondary antibodies were purchased from ZSGB-Bio (Beijing, China). The targeted proteins were visualized with the Super Signal West Femto Chemiluminescent Substrate (Thermo Scientific Pierce), and the intensities of the visualized bands were analyzed using Quantity One software (Bio-Rad). β-Actin (mouse monoclonal antibody, TA-09, from ZSGB-Bio in Beijing, China) was used as an internal control. The data are expressed as the ratio to β-Actin.

An equal amount of total protein was run on 10% SDS-PAGE (EpiZyme, cat. no. PG112), transferred to PVDF membranes (Merck millipore, cat. no. IPVH00010) (380 mA for 2 h), and probed with primary antibodies. The primary antibodies include anti-HBsAg (1:1000, 27 kDa, Novus Biologicals), anti-HBx (1:1000, 17 kDa, Abcam), anti-CK7 (1:1000, 51 kDa, Signalway antibody (SAB)), anti-CK19 (1:1000, 40 kDa, Signalway antibody (SAB)), anti-Hep-Par1 (1:1000, 165 kDa, Proteintech Group), anti-ALB (1:1000, 66 kDa, Proteintech Group), anti-α-Tubulin (1:1000, 55 kDa, Proteintech Group), anti-GAPDH (1:1000, 36 kDa, CST). The target protein bands were captured by binding of the secondary antibodies linked with peroxidase (1:1000, Anti-Rabbit IgG (H + L), CST) (1:1000, anti-mouse IgG (H + L), CST) to the primary antibodies.