A Biostat B plus MO5L fermenter (Sartorius Stedim Biotech GmbH, Göttingen, Germany) was used to perform fed-batch fermentation reactions using 2 L of fresh M9 medium (20 g glucose, 9.8 g KH2PO4, 2.1 g citric acid, 0.3 g ammonium ferric citrate, 5 g beef extract, 25 mg MgSO4·7H2O per 1 L water, pH 7.0) at 37 °C. After the initial carbon source (20 g L−1 glucose) was almost entirely consumed, a 3 M glucose solution was added to begin the fed batch mode. A 25% ammonia solution was used to maintain a constant neutral pH. Given that oxygen is an important factor for isoprenoid synthesis, fermentation was performed under strict aerobic conditions, with the dissolved oxygen concentration maintained at 20% saturation. When the OD600 reached 20, 0.05 mM IPTG was added to induce recombinant protein expression. Fresh IPTG and antibiotics were added every 24 h, and lycopene production and cell growth were monitored every 5 h throughout the 72 h fermentation process.
Biostat b plus mo5l fermenter
Manufactured by Sartorius
Sourced in Germany
The Biostat B plus MO5L fermenter is a laboratory-scale bioreactor system designed for microbial and cell culture applications. It features a working volume of 5 liters and is equipped with necessary controls and instrumentation for precise monitoring and regulation of key process parameters such as temperature, pH, dissolved oxygen, and agitation speed.
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Lab products found in correlation
2 protocols using biostat b plus mo5l fermenter
1
Fed-Batch Fermentation for Isoprenoid Production
2
Lycopene Production via Fed-batch Fermentation
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A Biostat B plus MO5L fermenter (Sartorius Stedim Biotech GmbH, Göttingen, Germany) was used to perform fed-batch fermentation reactions using 2 L of fresh M9 medium (20 g glucose, 9.8 g KH2PO4, 2.1 g citric acid, 0.3 g ammonium ferric citrate, 5 g beef extract, 25 mg MgSO4·7H2O per 1 L water, pH 7.0) at 37 °C. After the initial carbon source (20 g/L glucose) was almost entirely consumed, a 3 M glucose solution was added to begin the fed batch mode. A 25% ammonia solution was used to maintain a constant neutral pH. Given that oxygen is an important factor for isoprenoid synthesis, fermentation was performed under strict aerobic conditions, with the dissolved oxygen concentration maintained at 20% saturation. When the OD600 reached 20, 0.05 mM IPTG was added to induce recombinant protein expression. Fresh IPTG and antibiotics were added every 24 h, and lycopene production and cell growth were monitored every 5 h throughout the 72 h fermentation process [35 (link)].
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