Cd34 percp antibody

MV4-11 cells were incubated at 5 × 10⁵/ml in culture medium for four hours with Venetoclax (10 nM), AC220 (10 nM) or S63845 (2.5 nM/5 nM) or a combination of drugs. Primary AML samples were incubated at 1 × 10⁶/ml in culture medium for four hours with Venetoclax (100 nM), AC220 (100 nM) or S63845 (25 nM/50 nM) or a combination of drugs. To measure the percentage of cells with loss of cytochrome C, cells were fixed in 2% para-formaldehyde following 4-hour drug incubation. Fixed and rinsed cells were permeabilised with saponin and labelled with Alexa-647-conjugated cytochrome C antibody. Leukaemic blast cells were identified using CD45 APC-H7 antibody (Becton Dickinson). LSCs were identified using CD34 PerCP antibody (Becton Dickinson) and CD38 (AT1) PE antibody (Beckton Dickinson). Data were collected on a FACSCanto II flow cytometer (Becton Dickinson) and analysed with FACS Diva software (Becton Dickinson).