Linear regression analysis in PROC MIXED of
SAS for Windows 9.4 (SAS Institute, Inc., Cary, NC, USA) was used for growth kinetics, biofilm, hemolysis, and bioluminescence assays where the mutant strains were compared to
E. piscicida strain C07–087. Separate models were developed for each analytical test using manual forward selection, and each included mutant type and hour as fixed effects. Rep was included as a random effect. Adjustment for multiple comparisons were made using the SIMULATE option. Results are reported as least square means± standard error.
Exact logistic regression was used in PROC LOGISTIC of
SAS for Windows 9.4 (SAS Institute, Inc., Cary, NC, USA) to evaluate percent mortalities during the pathogenicity and vaccine trials. The outcome was the number of deaths over the total number of trials. For the challenge analysis, comparisons were made to the negative control, and separate models were developed for low and high doses. Odds ratios and 95% Confidence Limits are reported. For the pathogenicity analysis, comparisons were made to strain C07–087.
Edrees A., Abdelhamed H., Nho S.W., Park S.B., Karsi A., Austin F.W., Essa M., Pechan T, & Lawrence M.L. (2018). Construction and evaluation of type III secretion system mutants of the catfish pathogen Edwardsiella piscicida. Journal of fish diseases, 41(5), 805-816.