Allyl-alcohol (99%), benzyl alcohol (99%), butanol (99.5%), 1-octanol (99%), phenyl acetaldehyde (98%, stabilized) and trimethylamine (99.7%) were purchased from Acros Organics. 1-Adamantyl isocyanide (95%), cyclohexanol (99%), cyclohexyl isocyanide (98%), 2-butanol (99.5%, anhydrous), 2,4-dinitrobenzaldehyde (97%), isobutyraldehyde (≥99.5%), 2-morpholinoethyl isocyanide (≥98%), tert-butanol (≥99.5%, anhydrous), tert-butyl isocyanide (98%), tetradecane (≥99.5%) and undecylenic aldehyde (95%) were obtained from Sigma Aldrich. Other chemicals used include: diazabicyclo[5.4.0]undec-7-ene (DBU, TCl, >98%), deuterated chloroform (CDCI3-d, Merck), dimethyl sulfoxide (DMSO, VWR, 99%) and trimethyl acetaldehyde (ABCR, 97%). Carbon dioxide (CO2) with purity over 99.9% was obtained from Air Liquide. Cyclohexane, ethyl acetate and dichloromethane were distilled before usage, while tetrahydrofuran (THF) was of technical grade and used without further purification. All other chemicals were used as received from the supplier.
Butanol
Manufactured by Thermo Fisher Scientific
Sourced in United States, Belgium
Butanol is a four-carbon alcohol commonly used in various industrial applications. It serves as a versatile organic solvent and can be employed as a precursor for the synthesis of other chemical compounds. Butanol exhibits properties suitable for specific laboratory and industrial processes, though its precise applications may vary based on the specific requirements of the user.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Thermo Fisher Scientific (7 mentions)
Methanol, by Thermo Fisher Scientific (6 mentions)
Ethanol, by Thermo Fisher Scientific (4 mentions)
Sodium hydroxide (naoh), by Thermo Fisher Scientific (3 mentions)
Fm4 64, by Thermo Fisher Scientific (3 mentions)
Acetone, by Thermo Fisher Scientific (3 mentions)
Benzyl alcohol, by Thermo Fisher Scientific (2 mentions)
Chloroform, by Thermo Fisher Scientific (2 mentions)
Formic acid, by Thermo Fisher Scientific (2 mentions)
Hexane, by Thermo Fisher Scientific (2 mentions)
Phenylacetaldehyde, by Thermo Fisher Scientific (1 mentions)
Tetradecane, by Merck Group (1 mentions)
Allyl alcohol, by Thermo Fisher Scientific (1 mentions)
Trimethylamine, by Thermo Fisher Scientific (1 mentions)
Cyclohexyl isocyanide, by Merck Group (1 mentions)
Isobutyraldehyde, by Merck Group (1 mentions)
1 octanol, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Avantor (1 mentions)
2 butanol, by Merck Group (1 mentions)
Tert butyl isocyanide, by Merck Group (1 mentions)
16 protocols using butanol
1
Chemical Reagents for Organic Synthesis
2
Analytical Reagents and Standards
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The salts and supplements used to prepare the culture media were in general purchased from Sigma-Aldrich; except Dichloran-glycerol (DG18) agar, yeast extract and bacteriological peptone that were purchased from Oxoid; sucrose from Alfa Aesar; casein hydrolysate from Fluka; agar and NaCl from Panreac AppliChem; NaOH from J.M.G. Santos and glycerol from Fisher Bioreagents. Molecular biology reagents used for PCR reactions were purchased from NZYTech. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was purchased from Sigma-Aldrich. The reagents used for sphingolipid extraction: pyridine, butanol, ammonium hydroxide and 40% methylamine in water were purchased from Acros Organics; ethanol, methanol and chloroform (and dimethyl sulfoxide used in MTT assays) were purchased from Fisher Chemical and diethylether from Panreac AppliChem. Sphingoid bases standards (phytosphingosine, sphingosine and dihydrosphingosine) were purchased from Avanti Polar Lipids. All solvents used in chromatographic analyses were of the highest analytical grade and water was obtained from a Milli-Q system (Millipore).
3
Analytical Reagents for Phytochemical Analysis
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Methanol HPLC grade and acetonitril Far UV HPLC grade were purchased by Fisher Scientific (Hampton, NH, USA). Butanol (99%), formic acid (99+%), acetic acid (99.8%), sulfuric acid (85% g/g) and ethylacetate HPLC grade were provided by Acros (Geel, Belgium). Hederacoside C (98.19% purity and 97.70% purity) was obtained by Extrasynthese (Genay, Cedex, France) and Roth (Bonheiden, Belgium), rutin (99.70% purity) was purchased by Extrasynthese (Genay, Cedex, France) and Sigma (96% purity) (Bornem, Belgium).
Ursodeoxycholic acid (UDCA) was purchased in the form of Ursofalk ® 250 mg (Dr. Falk Pharma Benelux B.V.). Other chemicals included praziquantel (Droncit ® , Bayer), oxibendazole and myclozamide solution (Vitaminthe ® , BCI), marbofloxacin 2% (Marbocyl ® , Vétoquinol), acepromazin (Calmivet ® , Vétoquinol), atropin (Promopharm), tolfenamic acid 4% (Tolfidin ® , Vétoquinol), sodium thiopental (Nesdonal ® , Sanofi-Aventis), xylazine (Rompun ® , Bayer), ketamin (Clorketam 1000 ® , Vétoquinol) and isoflurane (Cooper Maroc).
A kit for cholesterol determination was obtained by DiaSYS Diagnostic Systems GmbH (Holzeim, Germany).
Ursodeoxycholic acid (UDCA) was purchased in the form of Ursofalk ® 250 mg (Dr. Falk Pharma Benelux B.V.). Other chemicals included praziquantel (Droncit ® , Bayer), oxibendazole and myclozamide solution (Vitaminthe ® , BCI), marbofloxacin 2% (Marbocyl ® , Vétoquinol), acepromazin (Calmivet ® , Vétoquinol), atropin (Promopharm), tolfenamic acid 4% (Tolfidin ® , Vétoquinol), sodium thiopental (Nesdonal ® , Sanofi-Aventis), xylazine (Rompun ® , Bayer), ketamin (Clorketam 1000 ® , Vétoquinol) and isoflurane (Cooper Maroc).
A kit for cholesterol determination was obtained by DiaSYS Diagnostic Systems GmbH (Holzeim, Germany).
4
Synthesis and Characterization of Silver Nanoparticles
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AgNO 3 (laboratory reagent grade) was obtained from Fisher Scientific. NaBH 4 (99%), (±)-α-lipoic acid (≥99%) and methanol (≥99.9%) were purchased from Sigma Aldrich. Butanol (99.5%) was obtained from Acros. Water was of Milli-Q quality, purified using a Millipore Direct-Q 3 water purification system.
5
Yeast Strain Generation by Homologous Recombination
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Deletion and GFP-fusion strains were obtained by homologous recombination of the PCR product with heterologous selection markers, as listed in Table 1 . All strains in this study are derivatives of the W303 strain. As templates for PCR, the DNA of yeast strains from Euroscarf collection, pUG27 (Gueldener et al., 2002 (link)) and pFA6a-His3MX6 (Longtine et al., 1998 (link)) plasmids were used. All newly generated strains were verified by PCR with independently designed primers (Table 2 ) and RT-qPCR (Supplementary Text S1 , Table S1 , Figure S1 ). We used standard yeast-rich and synthetic mediums described by Sherman (2002) (link). We obtained yeast extract from BD and D-glucose from Helicon. Clotrimazole, miconazole, nigericin, amphotericin B, propanol, butanol, and FM4–64 were obtained from Thermo Fisher Scientific. Bacto Agar, peptone, NaCl, KCl, and NaN3 were obtained from Amresco, and 2-Deoxy-D-glucose was obtained from Chem-Impex Int’l Inc.
6
Solvents and Water Purification for Analysis
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Methanol, acetonitrile, butanol, and dichloromethane were purchased from Thermo Fisher Scientific (Thermo Fisher, Waltham, MA, USA), and formic acid and ammonium acetate were from DiKMA Technologies (Beijing, China). Ultrapure water (18.2 MΩ) was prepared using a Milli-Q system (Millipore Billerica, Burlington, MA, USA).
7
Procurement of Analytical Reagents for Chemical Analyses
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Nile red, Nile blue A, and sodium hydroxide were purchased from Sigma-Aldrich Co. (Missouri, United States); 1,2-propanediol, hydrochloric acid, ammonium thiocyanate were obtained from Merck KGaA (Darmtstadt, Germany); potassium dihydrogen phosphate, sodium borate, sodium borohydride, sodium citrate dihydrate, barium chloride, ferrous sulfate, and ferrous iron were purchased from Sinopharm chemical reagent Co., Ltd (Shanghai, China); isopropanol, acetonitrile, pentane, ethyl acetate, hexane, isooctane, methol, and butanol were purchased from Thermo Fisher Scientific (Waltham, USA). Furosine*HCl was purchased from Iris Biotech GmbH (Marktredwitz, Germany); Nε-carboxymethyl-l -lysine was purchased from Toronto Research Chemicals Inc. (Toronto, Canada); l -lysine was purchased from Anpel Laboratory Technologies Inc. (Shanghai, China); FMOC N-hydroxysuccinimide ester (≥98 %, chromatographic grade) and Cumene hydroperoxide (80 %, technical grade), propanal (97 %) and hexanal (98 %) were purchased from Sigma-Aldrich Co. (Missouri, United States); Propionaldehyde-2,2,3,3,3,-d5 (98.7 %, chromatographic grade) and hexanal-d12 (98.5 %, chromatographic grade) were purchased from CDN isotopes Inc. (Quebec, Canada).
8
Comprehensive Analytical Methods for Plant Phenolics
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Ethanol, hydrochloric acid (37%), and acetone of HPLC grade were purchased from Scharlab Chemie (Barcelona, Spain). Gallic acid, Folin-Ciocalteu reagent, epicatechin, vanillin, polyethylene glycol (8000 Da), polyethylene glycol (4000 Da), twin20 (1228 Da), ethylene glycol (62 Da), dextran (50,000 Da), iron(III) chloride, sodium carbonate, hydrogen peroxide, 4-dimethylaminocinnamaldehyde (DMAC), 6-hydroxy-2,5,7,8-tetramethyl-chromane-2-carboxylic acid (Trolox), potassium persulfate, 2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) and diammonium salt (ABTS)were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Sodium dihydrogen phosphate dihydrate and dipotassium hydrogen phosphate were obtained from Merck (Hesse, Darmstadt, Germany).
MEthanol (99.99%), formic acid, acetonitrile, and butanol of HPLC grade were supplied by Fisher Scientific (Midlands, Leicestershire, UK). Ultrapure water (18.2 MΩ/cm) was obtained from a Millipore system (Millipore, Billerica, MA, USA). Promod 439 LTM enzyme was a kind donation of Biocatalysts Ltd. (Wales, Cardiff, UK).
MEthanol (99.99%), formic acid, acetonitrile, and butanol of HPLC grade were supplied by Fisher Scientific (Midlands, Leicestershire, UK). Ultrapure water (18.2 MΩ/cm) was obtained from a Millipore system (Millipore, Billerica, MA, USA). Promod 439 LTM enzyme was a kind donation of Biocatalysts Ltd. (Wales, Cardiff, UK).
9
Fabrication of Polyacrylonitrile-Polydimethylsiloxane Membranes
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Polyacrylonitrile (PAN) membranes, used as a support for PDMS in this study, were purchased from Synder Filtration (Vacaville, CA, USA) with a molecular weight cut-off of 30,000 Da and a thickness (Polyester + PAN) of 0.15 mm. PDMS and a cross-linking agent kit (RTV615 001-KIT) were obtained from Momentive Co. (Hebron, OH, USA). Super activated porous carbon nanopowder (US1074: Particle size 20–40 nm, with a pore size of 3.5 nm and specific surface area greater than 1400 m2/g), was purchased from US-Nano Company (South Bend, IN, USA). Commercial PDMS membranes with a total thickness of 200–235 μm (130, 100, 3–5 μm for Polyethylene terephthalate (PET), Polyimide (PI), and PDMS, respectively) were obtained from Pervatech B.V. Company (Rijssen, The Netherlands). Butanol (99% pure, Acros), acetone (95% pure, Acros), ethanol (99% pure, Acros), and toluene (99% pure, Acros) were obtained from Fisher Scientific (Fair Lawn, NJ, USA). Deionized distilled water was used to prepare all solutions.
10
Grape Pomace Extraction Method
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The grape pomace (GP) sample tested in the laboratory was collected from a red wine producer from the central region of Portugal after the fermentation of three Portuguese grape varieties (Trincadeira, Baga, and Alfrocheiro). The GP sample (ca. 10 kg) was dried at 40 °C until it reached a constant weight, milled (Retsch, model 5657, Germany) until particle size < 1 mm, and stored in a sealed and dry environment until further utilization. The solvents tested were ultrapure water (Direct-Pure Water System, Interlab, China), methanol (Carlo Erba Reagents, 99.9%, France), ethanol (Honeywell, 99.8%, Germany), acetone (Fisher Chemical, 99.8%, Belgium), and butanol ((Fisher Chemical, 99.5%).
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