2400 analyzer

All of the chemicals were obtained from commercial sources and used without further purification. Elemental (C, H and N) analyses were performed on a Perkin-Elmer 2400 analyzer. FT-IR data were collected on a Perkin-Elmer 100 spectrophotometer by using KBr pellets in the range of 4500− 450 cm⁻¹. Thermal analyses were carried out on a STA-6000 with a heating rate of 10 °C min⁻¹ in a temperature range from 30 °C to 800 °C in atmosphere. Powder X-ray diffraction (PXRD) data were recorded on a Rigaku D/Max-3B X-ray diffractometer with CuKα as the radiation source (λ  =  0.15406 nm) in the angular range θ  =  5− 50° at room temperature. GC− MS spectra were recorded on a SHIMADZU GCMS-QP2010. Nuclear magnetic resonance (NMR) was carried out on a Bruker AVANCE III 500 system. The concentration of dyes was analyzed by a UV-visible spectrophotometer (Perkin-Elmer 35), which recorded the temporal UV-visible spectral variations of the dyes with characteristics absorbance peak. Electron paramagnetic resonance (EPR) spectra were recorded on a EMX-10/12 spectrometer.

The chemicals were purchased from Sigma-Aldrich and used without purification. A Nicolet iS10 spectrometer from Thermo Scientific was used to record IR spectra (KBr). The ¹H NMR and ¹³C NMR spectra were recorded by JEOL's (500 MHz) and Bruker NMR spectrometers (400 MHz) in DMSO-d₆ and CDCl₃. Mass spectra was measured through Thermo Scientific GC/MS model ISQ. The Perkin-Elmer 2400 analyzer was used to obtain the elemental analysis of C, H, and N.

The metal precursors [PtCl₂(phen)] and [PdCl₂(phen)] used in the synthesis of complexes I and II were prepared as previously reported in the literature [52 (link),53 (link),54 (link)]. All reagents and solvents were purchased from Merck and were used as received. Elemental analyses to determine the percentage carbon, hydrogen, and nitrogen (CHN) were performed on a CHNSO PerkinElmer 2400 Analyzer. Infrared spectra (4000–220 cm⁻¹) were performed on a PerkinElmer Frontier MIR spectrometer equipped with an attenuated total reflectance (ATR) sample holder with a diamond crystal. Conductivity measurements were performed using a Tecnopon mCA-150 conductivity meter with UV/HPLC grade dimethyl sulfoxide as solvent. ¹H, ¹³C and ¹⁹⁵Pt NMR spectra were performed on a Bruker AscendTM 400 Avance III HD spectrometer (9.2 T) at 400 MHz (¹H), 100 MHz (¹³C), and 86 MHz (¹⁹⁵Pt) using DMSO-d₆ as solvent at room temperature. Chemical shifts were expressed as δ (in ppm) from the internal reference standard TMS (δTMS = 0.00) and K₂[PtCl₄] (¹⁹⁵Pt NMR).

Fourier transform infrared (FTIR) spectra of samples in film form or diluted in KBr as pellets were recorded from 4000 to 250 cm⁻¹ (2 cm⁻¹ resolution) with a FTIR spectrophotometer BRUKER IFS 66v/S. CHNS elemental chemical microanalysis of samples was determined in a Perkin-Elmer 2400 analyzer. Solid-state CP-MAS ¹³C NMR spectra of samples spun at 10 kHz were obtained in a Bruker Avance 400 spectrometer, using a contact time of 2 ms and a period between successive accumulations of 5 s. The number of scans was 800 and chemical shift values were referenced to tetramethylsilane. The qualitative analysis of protein fractions (λ = 250–600 nm) and the UV–vis transmittance (λ = 200–800 nm) of bionanocomposite films (rectangular shape, 2 cm × 4 cm) were determined using a Shimadzu UV-1201 spectrophotometer. Surface morphology was observed with a FE-SEM equipment FEI-NOVA NanoSEM 230, which allowed semi-quantitative analysis of elements. The equipment allows for the direct observation of samples adhered on a carbon tape without requirement of any conductive coating on the surface. For the TEM images (Philips Tecnai 20, operating at 200 kV), the biohybrids were previously embedded in epoxy resin and then cut in very thin sections using an ultramicrotome (LEICA EM UC6) equipped with a diamond blade.

Attenuated total reflection-Fourier transform infrared (ATR-FTIR)
spectra were collected on a Bruker VERTEX 70 optical spectrometer.
Elemental analyses (C, H, N) were measured on a PerkinElmer 2400 analyzer.
Thermogravimetric (TG) analysis (TGA) data were recorded on an SDT-Q600
analyzer from TA instruments. The temperature varied from room temperature
(RT) to 900 °C at a heating rate of 10 °C·min^–1 under air or N₂ flow. Raman spectra were collected with
a JASCO NRS-5100 Raman microscope using an excitation line of 532
nm (Nd:YAG laser), a power of 2.3 mW, and an acquisition time of 10
s. Scanning electron microscopy (SEM) was carried out on a FEI, Helios
Nanolab 650, with an energy-dispersive X-ray spectrometer. High-angle
annular dark-field scanning transmission electron microscopy (HAADF-STEM)
and high-resolution transmission electron microscopy (HRTEM) were
performed in a FEI Talos F200X. X-ray photoelectron spectroscopy (XPS)
analyses were performed on a Physical Electronics ESCA 5701 spectrometer.