Retinal ganglion cell projections from the right and the left eye were bulk labeled with CTB Alexa 488 and CTB-Alexa 555. The tracer was diluted to 1 mg/ml in 0.9% saline. At P12/13, mice were anesthetized and injected with 1–2 μL tracer per eye using a glass pulled pipette and Nanoject (Drummond Scientific, Broomall, PA). 48 hours later mice were transcardially perfused and the brains were fixed overnight in 4% PFA. Coronal sections (80 μm thickness) were collected with a vibratome as described above, mounted in Aquamount and imaged with a CCD camera (Zeiss). Analysis of segregation of contralateral and ipsilateral projections in dLGN was performed as previously described (Torborg and Feller, 2004 (link)). Briefly, images were background subtracted with a rolling ball radius of 200 in ImageJ, and the three sections with the largest ipsilateral (Alexa 555 labeled) area were used for analysis. The logarithm of the intensity ratio, R = log10 (ipsilateral channel fluorescence intensity/contralateral channel fluorescence intensity), was determined for each pixel, and a segregation index for each animal was computed as the mean of the variance of the distribution of R values. A larger segregation index (higher variance) is indicative of better segregation (Torborg and Feller, 2004 (link)).
Ccd camera
Manufactured by Zeiss
Sourced in Germany, United States, Japan
The CCD camera is a type of image sensor that converts light into an electrical signal. It is designed to capture high-quality images and is commonly used in various scientific and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Axiovision software, by Zeiss (8 mentions)
Axio observer microscope, by Zeiss (5 mentions)
Axioscope microscope, by Zeiss (5 mentions)
Axio observer d1 microscope, by Zeiss (3 mentions)
Triton x 100, by Merck Group (3 mentions)
Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (3 mentions)
Image pro plus 6, by Media Cybernetics (3 mentions)
Axio observer a1, by Zeiss (3 mentions)
Rhodamine phalloidin, by Thermo Fisher Scientific (3 mentions)
Lsm 710 confocal microscope, by Zeiss (3 mentions)
Nanoject, by Drummond (2 mentions)
Zen 2, by Zeiss (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions)
Non specific polyclonal rabbit antibody, by Abcam (2 mentions)
Ht90132, by Zeiss (2 mentions)
Stemi 2000 c stereomicroscope, by Zeiss (2 mentions)
Anti rabbit igg, by BD (2 mentions)
Anti mouse igg alexa fluor 488, by BD (2 mentions)
Anti fibronectin mouse monoclonal antibody, by Santa Cruz Biotechnology (2 mentions)
Axioskop 40, by Zeiss (2 mentions)
78 protocols using ccd camera
1
Mapping Retinal Ganglion Cell Projections
2
Mapping Retinal Ganglion Cell Projections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Retinal ganglion cell projections from the right and the left eye were bulk labeled with CTB Alexa 488 and CTB-Alexa 555. The tracer was diluted to 1 mg/ml in 0.9% saline. At P12/13, mice were anesthetized and injected with 1–2 μL tracer per eye using a glass pulled pipette and Nanoject (Drummond Scientific, Broomall, PA). 48 hours later mice were transcardially perfused and the brains were fixed overnight in 4% PFA. Coronal sections (80 μm thickness) were collected with a vibratome as described above, mounted in Aquamount and imaged with a CCD camera (Zeiss). Analysis of segregation of contralateral and ipsilateral projections in dLGN was performed as previously described (Torborg and Feller, 2004 (link)). Briefly, images were background subtracted with a rolling ball radius of 200 in ImageJ, and the three sections with the largest ipsilateral (Alexa 555 labeled) area were used for analysis. The logarithm of the intensity ratio, R = log10 (ipsilateral channel fluorescence intensity/contralateral channel fluorescence intensity), was determined for each pixel, and a segregation index for each animal was computed as the mean of the variance of the distribution of R values. A larger segregation index (higher variance) is indicative of better segregation (Torborg and Feller, 2004 (link)).
3
ADPKD Kidney Receptor Immunohistochemistry
4
Melanoma Cell Migration Assay with CAF/NHF
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Melanoma cells were plated on 35 mm dishes and allowed to grow until confluence. To obtain a standardized cell-free area, the cell monolayer was then wounded with a pipette tip (1 mL), washed, and maintained in CAF or NHF supernatant diluted 1:1 with culture medium for 24, 48, and 72 h. The cultures were fixed immediately after the scratch (T0), at 24, 48, and 72 h. Images were recorded using a CCD camera (Zeiss, Oberkochen, Germany) and the migratory ability was quantified by measuring the leading edge distance using the Zen 2.6 software (Zeiss). Results are expressed as the percentage of reduction with respect to T0, which was set as 100. For each time point evaluated, the reduction of edge distance of melanoma cells treated with NHF supernatant was compared with that of the cells maintained with CAF supernatant.
5
Immunofluorescence Staining of Cultured Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After fixation in 4% Paraformaldehyde/4% Sucrose at room temperature for 15 min, the cells were washed with PBS and permeabilized with 0.2% Triton X-100 in PBS for 5 min twice. Subsequently, blocking solution (BS; 10% FBS in PBS) was applied at room temperature for 1 h. Cells were incubated with the primary antibodies at 4 °C over night. After washing 3× with PBS, the cells were incubated with Alexa Fluor conjugated secondary antibody diluted 1:1000 in BS at room temperature for 1 h. After washing 2× with PBS and 1× with sterile Millipore water, cells were mounted with ProLong® Gold antifade reagent with DAPI. Fluorescence images were obtained an upright Axioscope microscope equipped with a Zeiss CCD camera (16 bits; 1280 × 1024 ppi) using Axiovision software (Zeiss) and analyses of integrated densities was performed with ImageJ 1.50 g.
6
Immunocytochemistry of α-SMA and FAP
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells on coverslips were fixed with 4% paraformaldehyde for 15 min at room temperature followed by 0.1% Triton X-100 to allow cell permeabilization. Cells were then incubated with anti-α-SMA monoclonal (Sigma Aldrich, Merck Life Science S.r.l. Milan, Italy), or anti-FAP rabbit (Cohesion Bioscience, London, UK) for 1 h. Primary antibodies were visualized using an anti-mouse or anti-rabbit IgG Alexa Fluor 488 (BD Biosciences, Milan, Italy). Fluorescence signals were recorded using a CCD camera (Zeiss, Oberkochen, Germany).
7
Immunofluorescence Staining with Paraformaldehyde
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were fixed with 4% paraformaldehyde (PFA)/4% sucrose/PBS at 4°C for 20 min. After washing 2× 5 min with 1× PBS with 0.2% Triton X-100 at RT, blocking was performed with 10% FBS in 1× PBS at RT for 1 h, followed by the primary antibody for 2 h at RT. After a 3× 5 min washing-step with 1× PBS, incubation with the secondary Alexa488 and/or Alexa568 antibody followed for 1 h at RT. The cells were washed again in 1× PBS for 10 min and cell nuclei stained with DAPI for 5 min. After washing with aqua bidest., coverslips were mounted using VectaMount (Vector Labs). Fluorescence images were obtained using an upright Axioscope microscope equipped with a Zeiss CCD camera (16 bits; 1280 × 1024 ppi) using Axiovision software (Zeiss) and ImageJ 1.51j.
8
Immunohistochemical Analysis of Complex I
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serial sections derived from formalin-fixed and paraffin-embedded blocks were de-waxed in xylene and rehydrated through a graded series of ethanol. Tissue sections were incubated with mouse MoAb anti-Complex I 15 kDa (1:50; Molecular Probes, Life Technologies) and then visualized using a goat anti mouse-Texas Red 1:100 (Santa Cruz Biotechnology Inc.). Nuclei were counterstained with 4′,6′-diamidino-2-phenylindole (DAPI) (Sigma Aldrich). Fluorescence signals were analyzed using stained images with a CCD camera (Zeiss). The analysis was performed at 63x and 100x, and the results are shown here at 100x. Quantitative analysis of CxI fluorescence intensity was performed using AxioVision 4.7.1 software (Zeiss). The results were expressed as the fold increase of the fluorescence intensity reported as the mean value ± SD relative to the healthy skin, which was set as 1 by definition.
9
Quantifying Polyglutamine Aggregates
Check if the same lab product or an alternative is used in the 5 most similar protocols
unc‐54p::Q35::YFP(rmIs132)‐expressing day 3 adult animals were immobilized in 0.1 µm diameter polystyrene microspheres (Polysciences) on 2% agarose pads and were imaged using the 10× objective on a Zeiss Axio Observer microscope equipped with an Andor Clara CCD camera. Metamorph software was used to compile the images. The number of polyQ aggregates was counted, each aggregate determined as a structure fully discernible from any surrounding aggregates.
+ Expand
unc‐54p::Q35::YFP(rmIs132)‐expressing day 3 adult animals were immobilized in 0.1 µm diameter polystyrene microspheres (Polysciences) on 2% agarose pads and were imaged using the 10× objective on a Zeiss Axio Observer microscope equipped with an Andor Clara CCD camera. Metamorph software was used to compile the images. The number of polyQ aggregates was counted, each aggregate determined as a structure fully discernible from any surrounding aggregates.
10
Histological Sectioning of Maize Kernels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The histological section of developing kernels staged at 8, 10, 12, and 14 DAP were prepared according to Zhao et al. (2018) (link) with minor modification. The kernels were harvested freshly from the middle of the ear and immersed immediately in the formaldehyde–acetic acid fixative containing 50% (v/v) ethanol, 5% glacial acetic acid, and 3.7% (v/v) formaldehyde at 4°C overnight. Then, the samples were dehydrated through a gradient of ethanol, xylene, and embedded in paraffin. Samples were sectioned with a microtome at 8 μm in thickness (Leica Microsystems, Wetzler, Germany), stained with toluidine blue O (TBO), and pictured by Zeiss microscopy (Jean, Germany) with a CCD Camera.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!