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Dylight fluorophore

Manufactured by Thermo Fisher Scientific
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DyLight fluorophores are a series of fluorescent dyes developed by Thermo Fisher Scientific. They are designed to be used as labels in various biological and analytical applications, such as immunoassays, flow cytometry, and fluorescence microscopy. DyLight fluorophores are available in a range of excitation and emission wavelengths, allowing for flexibility in experimental design.

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Lab products found in correlation

Horseradish peroxidase, by Thermo Fisher Scientific (1 mentions) Ha ha 7 h9658, by Merck Group (1 mentions) Sab4301135, by Merck Group (1 mentions) Prolong gold with dapi, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647 phalloidin, by Thermo Fisher Scientific (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Cyclohexane, by Merck Group (1 mentions)

4 protocols using dylight fluorophore

1

Antibody Characterization for Yeast Protein Detection

Cited 2 times
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Mouse monoclonal antibodies against the FLAG (M2; F3165) and HA (HA-7; H9658) tags were from Sigma-Aldrich. Rabbit polyclonal antibodies against the FLAG (SAB4301135) and HA (51064-2-AP) tags were from Sigma-Aldrich and Proteintech, respectively. Other antibodies include the anti-Aac2 monoclonal antibodies 6H8 and 2C10 (65 (link)), rabbit anti-Tom70 (66 (link)), anti-Atp1/2 (67 (link)), anti-Por1 (68 (link)), anti-Kgd1 and anti-Cor2 (69 (link)), anti-Aac2 (19 (link)), anti-Cox1 (70 (link)), anti-Cox2 (71 (link)), anti-Cox4, anti-Rip1, and anti-Qcr6 (72 (link)), anti-Om45 (73 (link)), and horseradish peroxidase–conjugated (Figs. 1; 2F; 3B; 4; 5, A and F; and 6, A and E; and figs. S1 and S2) and DyLight fluorophore–conjugated (Figs. 2, B and E; 5, D and E; and 6D) secondary antibodies (Thermo Fisher Scientific).
Senoo N., Kandasamy S., Ogunbona O.B., Baile M.G., Lu Y, & Claypool S.M. (2020). Cardiolipin, conformation, and respiratory complex-dependent oligomerization of the major mitochondrial ADP/ATP carrier in yeast. Science Advances, 6(35), eabb0780.
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2

Fluorescent Labeling of Amino-Functionalized Mesoporous Silica Nanoparticles

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A suspension of surfactant-free MSN-NH2 (5 mg) in ethanol (1 mL) was sonicated with an ethanolic solution of 3 mg/mL DyLight™ fluorophore (594 or 633; Thermo Fisher Scientific) and kept under gentle stirring overnight at room temperature. Next, the suspension was centrifuged (20,000 rcf) and washed with pure ethanol until the supernatant became clear (approximately 3 times). The labeled MSN were stored in ethanol at 4 °C in the dark.
Noureddine A., Paffett M.L., Franco S., Chan A.E., Pallikkuth S., Lidke K, & Serda R.E. (2021). Endolysosomal Mesoporous Silica Nanoparticle Trafficking along Microtubular Highways. Pharmaceutics, 14(1), 56.
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3

Fluorescent Mesoporous Silica Nanoparticle Synthesis

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Mesoporous silica nanoparticles (MSN; 200 nm particle size with 4 nm pore) were purchased from Sigma–Aldrich or fabricated in house, as previously described [62 (link)]. NHS-ester-activated DyLight fluorophores (ThermoFisher) were conjugated to propylamine functionalized MSN using 50−65 µg DyLight for every 2.5 mg MSN in ethanol at 10 mg/mL, with a one-hour incubation at room temperature with rotation.
Franco S., Noureddine A., Guo J., Keth J., Paffett M.L., Brinker C.J, & Serda R.E. (2020). Direct Transfer of Mesoporous Silica Nanoparticles between Macrophages and Cancer Cells. Cancers, 12(10), 2892.
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4

Fluorescent Labeling of Cellular Components

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Absolute (100%) and 95% ethanol (EtOH) were obtained from Decon Labs. Hydrochloric acid (HCl, 37%), ammonium nitrate (NH4NO3), cetyltrimethylammonium chloride (CTAC; 25 wt.% in H2O), cyclohexane, tetraethyl orthosilicate (TEOS), triethanolamine (TEOA), and (3-aminopropyl)triethoxysilane (APTES) were purchased from Millipore Sigma (Burlington, MA, USA). DyLight fluorophores, LysoTracker® Red DND-99, Alexa Fluor 647 phalloidin, and Prolong Gold with DAPI were purchased from Thermo Fisher Scientific (Grand Island, NY, USA).
Noureddine A., Paffett M.L., Franco S., Chan A.E., Pallikkuth S., Lidke K, & Serda R.E. (2021). Endolysosomal Mesoporous Silica Nanoparticle Trafficking along Microtubular Highways. Pharmaceutics, 14(1), 56.
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