C57Bl/6 Myd88LoxP/LoxP mice (Jackson Laboratories) were crossed to C57Bl/6 CD4-Cre animals (Taconic) to produce Myd88+/+; CD4-Cre+ mice (WT) and Myd88LoxP/LoxP; CD4-Cre+ (T-MyD88−/−) animals. Age-matched male mice were used to compare the spontaneous weight phenotype, including immune and microbiota responses, on a standard diet. Age-matched male and female mice were used to compare the weight phenotype, including immune and microbiota responses, on a high-fat diet (HFD). To measure T cell-dependent shaping of the microbiota, 4-week old Tcrb−/− mice (Jackson Laboratories) were used. To investigate Desulfovibrio desulfuricans-dependent shaping of the microbiota, 6-week-old WT C57Bl/6 mice (Jackson Laboratories) were used or age-matched CD4-Cre+ (WT) mice from our facility were used. To measure microbiota effects on weight gain in immunodeficient mice, 4-week old Rag1−/− mice (Jackson Laboratories) were used. GF mice were maintained in sterile isolators and verified monthly for GF status by plating and PCR of feces. GF C57Bl/6 animals were used in this study. The use of animals in all experiments was in strict adherence to federal regulations as well as the guidelines for animal use set forth by the University of Utah Institutional Animal Care and Use Committee.
Tcrb mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
Tcrb−/− mice are genetically modified mice that lack the T cell receptor beta (Tcrb) gene. These mice are a useful tool in immunological research as they lack mature αβ T cells, which are important for adaptive immune responses.
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Lab products found in correlation
4 protocols using tcrb mice
1
Microbiome Modulation by T Cell Immunity
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Generation of STAT3-deficient OT-II Mice
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C57BL/6 mice were purchased from Orient (Gyeonggido, Republic of Korea). OT-II, B6.SJL (CD45.1) and Tcrb−/− mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA). STAT3flox/floxCD4-Cre mice were kindly provided by Drs. C. Dong (MD Anderson Cancer Center, Houston, TX, USA) and S. Akira (Osaka University, Osaka, Japan) and were crossed with OT-II to generate T cell-specific STAT3-deficient OT-II mice. All mice were maintained in the specific pathogen free facility at the vivarium of Seoul National University. All animal experiments were performed using a protocol approved by Institutional Animal Care and Use Committee Seoul National University (SNU-140602-2-2).
3
Purchasing and Maintaining C57BL/6 and Tcrb-/- Mice
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C57BL/6 mice within the ages of 6-10 weeks were purchased from Orient Bio (Gyeonggi, Republic of Korea). Tcrb-/- mice were purchased from Jackson Laboratories (Maine, USA). Mice were maintained under semi-specific-pathogen-free animal facility in sterile, individually ventilated cages at the Seoul National University with free access to sterile water. CO2 inhalation was used for euthanasia.
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Genetically Modified Mice for Immunology Research
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