Random plaque sections were prepared by conventional histologic methods and stained with hematoxylin/eosin, Masson's trichrome, and immunohistochemical anti-CD68 staining (Ventana Medical Systems, Inc., Tucson, AZ). Fibrous cap, necrotic core, angiogenesis, macrophage content, plaque hemorrhage and calcification were semi-quantitatively assessed by a blinded vascular pathologist.
Anti cd68
Manufactured by Roche
Anti-CD68 is a laboratory reagent used for the detection and quantification of CD68, a glycoprotein expressed by monocytes and macrophages. This product can be used in various immunological and histological techniques, such as flow cytometry and immunohistochemistry, to study the presence and distribution of CD68-positive cells in biological samples.
Automatically generated - may contain errors
3 protocols using anti cd68
1
Histological Plaque Characterization
2
Frozen Muscle Tissue Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh muscle specimens were oriented, snap-frozen in isopentane-cooled liquid nitrogen and submitted for frozen section histology and routine enzyme histochemical staining, including acid phosphatase and alkaline phosphatase. Immunohistochemical staining for major histocompatibility complex class I (anti-MHC1, US Biological, Salem, MA, M3886-10) and anti-CD68 (Ventana, Tucson, AZ) were performed on frozen sections in some inflammatory cases. Additional segments of skeletal muscle were received isometrically fixed in 10% formalin and processed for paraffin embedding, hematoxylin and eosin (H&E) histology, Masson’s trichrome and Congo red (Sigma–Aldrich, St Louis, MO) stains; an average of 4 1–2-mm-blocks of fixed muscle were post-fixed in buffered glutaraldehyde and embedded in Epon-araldite for resin histology and electron microscopy.
3
Quantified Immunohistochemical Analysis of Carotid Artery
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Deparaffinized slides were treated with the following monoclonal antibodies: Ventana-prediluted monoclonal anti-CD45 (Ventana), anti-CD68 (Ventana), anti-α-smooth muscle actin (Ventana), anti-CD3 (Roche), anti-CD4 (Roche), anti-CD8 (Dako). Antibody incubations and staining were performed on the automated Ventana BenchMark(R) XT slide stainer. Sections were counterstained with H&E. As negative controls, the same procedures were performed without the primary antibodies. All slides were read independently by two investigators. The percentage of immunopositive cells per total number of cells, for each antibody, was determined under a microscope at 400× magnification, and averaged on three sections for carotid artery.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!