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On targetplus mcl 1 sirna

Manufactured by Thermo Fisher Scientific
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The ON-TARGETplus Mcl-1 siRNA is a laboratory reagent designed to target and silence the expression of the Mcl-1 gene. It is intended for use in gene knockdown studies and research applications.

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Lab products found in correlation

On targetplus non targeting pool, by Thermo Fisher Scientific (2 mentions) Smartpool, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

2 protocols using on targetplus mcl 1 sirna

1

Silencing Usp9X, Bag-3, and Mcl-1 in Cells

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SignalSilence® Usp9X siRNA I #6308 was purchased from CST. Non-targeting siRNA-pool (ON-TARGETplus Non-targeting Pool, # D-001810-10-05) and siRNA against Bag-3 (SMARTpool: ON-TARGETplus Bag3 siRNA, L-011957-00-0005) and Mcl-1 (SMARTpool: ON-TARGETplus Mcl-1 siRNA, L-004501-00-0005) were purchased from Thermo Fisher Scientific (Pittsburgh, PA) and transfected as previously described [38 (link)].
Karpel-Massler G., Shu C., Chau L., Banu M., Halatsch M.E., Westhoff M.A., Ramirez Y., Ross A.H., Bruce J.N., Canoll P, & Siegelin M.D. (2015). Combined inhibition of Bcl-2/Bcl-xL and Usp9X/Bag3 overcomes apoptotic resistance in glioblastoma in vitro and in vivo. Oncotarget, 6(16), 14507-14521.
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2

siRNA Knockdown of Usp9X, Bag3, PMAIP1, Mcl-1

Cited 1 time
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SignalSilence® Usp9X siRNA I #6308 was purchased from CST. Non-targeting siRNA-pool (ON-TARGETplus Non-targeting Pool, # D-001810-10-05) and siRNA against Bag3 (SMARTpool: ON-TARGETplus Bag3 siRNA, L-011957-00-0005), PMAIP1 siRNA and Mcl-1 (SMARTpool: ON-TARGETplus Mcl-1 siRNA, L-004501-00-0005) were purchased from Thermo Fisher Scientific (Pittsburgh, PA) and transfected as previously described [18 (link), 54 (link)]. Briefly, cells were incubated for 6 h with the formed complexes of Lipofectamine® 2000 (Invitrogen, Carlsbad, CA, U.S.A.) and the respective siRNA (12-well condition) in DMEM without FBS and antibiotics. After 6 h, FBS was added to a total concentration of 1.5%.
Karpel-Massler G., Bâ M., Shu C., Halatsch M.E., Westhoff M.A., Bruce J.N., Canoll P, & Siegelin M.D. (2015). TIC10/ONC201 synergizes with Bcl-2/Bcl-xL inhibition in glioblastoma by suppression of Mcl-1 and its binding partners in vitro and in vivo. Oncotarget, 6(34), 36456-36471.
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