129 svimj

Manufactured by Jackson ImmunoResearch

Sourced in Montenegro, United States

129/SvImJ is a mouse strain that can be used for various research purposes. It serves as a genetic background for generating genetically modified mouse models. The 129/SvImJ strain is commonly used in the development and study of transgenic and knockout mice.

Automatically generated - may contain errors

Lab products found in correlation

9 protocols using 129 svimj

Phenylhydrazine-Induced Anemia in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

129/SvImJ (wild-type), 129S4/SvJae (wild-type), or 129S4/SvJae-Pparα^tm1Gonz/J female mice (The Jackson Laboratory) 6 – 8 weeks of age, randomized by weight, were pretreated with GW7647 (100 μg/kg) for 3 days (days −3 – −1). On day 0, the mice were injected with phenylhydrazine (PHZ) (60 mg/kg). GW7647 injection was continued during days 0 – 6. Whole blood samples were collected at each day of day 1 – 5, and day 7 and 9 for Complete Blood Count (CBC) analyses.
4 to 6 week old Nan/+ mutant mice (officially designated as Klf1^Nan; http://www.informatics.jax.org/allele/MGI:1861107), obtained from the Jackson Laboratory, were injected with GW7647 (100 μg/kg) for 18 days. Mice were randomized by weight. Whole blood samples were collected every 3 days for CBC analyses.
All mouse procedures were approved by the Animal Care and Use Committees of the Massachusetts Institute of Technology (Cambridge, MA).

Lee H.Y., Gao X., Barrasa M.I., Li H., Elmes R.R., Peters L.L, & Lodish H.F. (2015). PPARα and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal. Nature, 522(7557), 474-477.

+ Open protocol

+ Expand

Body Composition Analysis in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

All in vivo experiments were carried out with male A/J,
C57BL/6J, AKR/J, DBA/2J, 129SVImJ and (B6 × DBA/2J)F1 mice purchased
from the Jackson Laboratory (Bar Harbor, ME). Mice were maintained in a
temperature-controlled room (23°C) with a 12-h light/12-hr dark cycle.
The mice were reared under conventional conditions and fed PicoLab Rodent Diet
20 (Lab Diet; 13% kcal fat) until 16 weeks of age. A subset of mice was fed high fat diet (D12331; 58 kcal% fat) for 8 weeks (8–16 weeks of age). Body composition (body fat, lean mass and free fluid) was
analyzed by Minispec NMR (Bruker) which uses the contrasting hydrogen density
and/or hydrogen spin properties from adipose tissue and muscle for estimating
body composition. A quality control check of NMR parameters using a standard
provided by the manufacturer was performed at the beginning of each day of
testing. All animal experiments were approved by the Pennington Biomedical
Research Center and Maine Medical Center Research Institute Institutional Animal
Care and Use Committees and in accordance with National Institutes of Health
guidelines for care and use of laboratory animals.

Anunciado-Koza R., Higgins D.C, & Koza R.A. (2015). Adipose tissue Mest and Sfrp5 are concomitant with variations of adiposity among inbred mouse strains fed a non-obesogenic diet. Biochimie, 124, 134-140.

+ Open protocol

+ Expand

Mouse Strain Comparison for Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6J and 129/SvImJ (henceforth 129/SvImJ) mice were from The Jackson Laboratory (Bar Harbor, ME) and used at a minimum of 10 weeks old, with approval from the VCU institutional Animal Care and Use Committee.

Kolawole E.M., McLeod J.J., Ndaw V., Abebayehu D., Barnstein B.O., Faber T., Spence A.J., Taruselli M., Paranjape A., Haque T.T., Qayum A.A., Wijesinghe D.S., Sturgill J.L., Chalfant C.E., Straus D.B., Oskeritzian C.A, & Ryan J.J. (2016). Fluvastatin Suppresses Mast Cell and Basophil IgE Responses: Genotype-Dependent Effects. Journal of immunology (Baltimore, Md. : 1950), 196(4), 1461-1470.

+ Open protocol

+ Expand

Phenylhydrazine-Induced Anemia in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Genetic Diversity in Mouse Models

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mice used in this study were F1 hybrids of C57Bl/6J reference females with wild-derived strains 129/SvImJ (B6x129), C3H/HeJ (B6xC3H), CAST/EiJ, (B6xCAST), PWK/PhJ (B6xPWK), and SPRET/EiJ (B6xSPRET). 129/SvImJ and C3H/HeJ hybrid females were acquired from the Jackson Laboratory (8 weeks old, housed 4/cage). CAST/EiJ, PWK/PhJ, SPRET/EiJ inbred males were acquired from the Jackson Laboratory and were bred to C57Bl/6J female mice at FHCRC. Mice were maintained on a 12-h light, 12-h dark schedule with lights turned on at 7 a.m. The housing room was maintained at 20–24 °C with 30–70% relative humidity. Mice have housed in individually ventilated cages (Allentown) with 75 square inches of floor space and 60 air changes/hour. Biofresh cage bedding was (Absortion Corp) at 1/8 inch depth and autoclaved on site. Water and Purina 5053 (irradiated) were available ad libitum. Nestlet material (Envigo’s diamond twist 7979C, also irradiated) was present in each cage for enrichment. Autoclavable certified igloos (Bio-serv) were provided in some cages. Mice were housed in a barrier facility that is AAALAC accredited. Mice were sacrificed at 8 weeks of age by CO₂ asphyxiation. All work was approved by the Institutional Animal Care and Use Committee (IACUC) of the Fred Hutchinson Cancer Research Center (FHCRC).

Halow J.M., Byron R., Hogan M.S., Ordoñez R., Groudine M., Bender M.A., Stamatoyannopoulos J.A, & Maurano M.T. (2021). Tissue context determines the penetrance of regulatory DNA variation. Nature Communications, 12, 2850.

+ Open protocol

+ Expand

Diverse Mouse Strains for Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6J (stock number 0664), A/J (stock number 0646), 129SvImJ (stock number 02448), NZO/HiLtJ (stock number 02105), NOD/ShiLtJ (stock number 01976), WSB/EiJ (stock number 01145), PWK/PhJ (stock number 3715), and CAST/EiJ (stock number 0928) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA), and Collaborative Cross strains CC001, CC002, CC019, and CC042 were purchased from the University of North Carolina (Chapel Hill, NC, USA). Male mice were 8 to 12 weeks old at the start of all experiments. Mice were housed under specific-pathogen-free conditions and in accordance with the University of Massachusetts (UMASS) Medical School IACUC guidelines.

Smith C.M., Proulx M.K., Olive A.J., Laddy D., Mishra B.B., Moss C., Gutierrez N.M., Bellerose M.M., Barreira-Silva P., Phuah J.Y., Baker R.E., Behar S.M., Kornfeld H., Evans T.G., Beamer G, & Sassetti C.M. (2016). Tuberculosis Susceptibility and Vaccine Protection Are Independently Controlled by Host Genotype. mBio, 7(5), e01516-16.

+ Open protocol

+ Expand

Mouse Strain Acquisition and Breeding

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6N mice were obtained from Charles Rivers and bred in-house. C57BL/6NJ (stock #005304), C57BL/6J (stock #000664), 129SvImJ (stock #002448), and NZB/BlNJ (stock #000684) mice were obtained from Jackson Laboratories. C57BL/6J mice were bred-in house. Mice were housed in SPF conditions and care was provided in accordance with Yale University IACUC guidelines (protocol #10365).

Treger R.S., Pope S.D., Kong Y., Tokuyama M., Taura M, & Iwasaki A. (2019). The lupus susceptibility locus Sgp3 encodes the suppressor of endogenous retrovirus expression SNERV. Immunity, 50(2), 334-347.e9.

+ Open protocol

+ Expand

Comparative Mouse Strain Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6J, 129/SvImJ, BALB/cJ, and B6.129(Cg)-Slc6a4tm1Kpl/J (SERT KO) female mice were purchased from Jackson Laboratory, bred and maintained at the VCU animal facilities. Experiments using mice employed animals housed in the same room and compared age- and sex-matched mice. All animal use was approved by the Virginia Commonwealth University Institutional Animal Care and Use Committee (IACUC).

Lemaire S., Keryer E., Stein M., Schepens I., Issakidis-Bourguet E., Gérard-Hirne C., Miginiac-Maslow M, & Jacquot J.P. (1999). Heavy-Metal Regulation of Thioredoxin Gene Expression in Chlamydomonas reinhardtii. Plant Physiology, 120(3), 773-778.

+ Open protocol

+ Expand

Generating F1 Hybrid Mouse Strains for Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mice used in this study were F1 hybrids of C57Bl/6J reference females with wild-derived strains 129/SvImJ (B6x129), C3H/HeJ (B6xC3H), CAST/EiJ, (B6xCAST), PWK/PhJ (B6xPWK), and SPRET/EiJ (B6xSPRET). 129/SvImJ and C3H/HeJ hybrid females were acquired from the Jackson Laboratory (8 week old, housed 4/cage). CAST/EiJ, PWK/PhJ, SPRET/EiJ inbred males were acquired from the Jackson Laboratory and were bred to C57Bl/6J female mice at FHCRC. Mice were maintained on a 12-h light, 12-h dark schedule with lights turned on at 7 a.m. The housing room was maintained at 20-24 °C with 30-70% relative humidity. Mice were housed in individually ventilated cages (Allentown) with 75 square inches of floor space and 60 air changes/hour. Biofresh cage bedding was (Absortion Corp) at 1/8 inch depth and autoclaved on site. Water and Purina 5053 (irradiated) were available ad libitum. Nestlet material (Envigo's diamond twist 7979C, also irradiated) were present in each cage for enrichment. Autoclavable certified igloos (Bio-serv) were provided in some cages. Mice were housed in a barrier facility that is AAALAC accredited. Mice were sacrificed at 8 wks of age by CO2 asphyxiation. All work was approved by the Institutional Animal Care and Use

Halow J., Byron R., Hogan M.S., Ordóñez R., Groudine M., Bender M.A., Stamatoyannopoulos J., & Maurano M.T. (2020). Tissue context determines the penetrance of regulatory DNA variation.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!