Depc treated water

Manufactured by Quality Biological

DEPC treated water is a type of laboratory-grade water that has been treated with diethyl pyrocarbonate (DEPC) to inhibit the activity of RNase enzymes. It is commonly used in molecular biology applications where the prevention of RNA degradation is critical, such as in the preparation of samples for RNA extraction, RT-PCR, and other RNA-based experiments.

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Lab products found in correlation

Pfu ultra dna polymerase, by Agilent Technologies (1 mentions) Guanosine 5 triphosphate, by Merck Group (1 mentions) T7 dna ligase, by New England Biolabs (1 mentions) 2 deoxyguanosine 5 triphosphate dgtp, by Promega (1 mentions) 2 deoxyuridine 5 triphosphate dutp, by Roche (1 mentions) T7 rna polymerase, by New England Biolabs (1 mentions) T4 polynucleotide kinase, by New England Biolabs (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Rnalater, by Thermo Fisher Scientific (1 mentions) Ethylene diamine tetraacetic acid (edta), by Corning (1 mentions) Ultrapure dnase rnase free distilled water, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) Dynabead, by Thermo Fisher Scientific (1 mentions) Sodium azide, by Merck Group (1 mentions) Mirneasy mini kit, by Qiagen (1 mentions) Tris hcl, by Quality Biological (1 mentions)

3 protocols using depc treated water

Radiolabeled Nucleotide Synthesis Protocol

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2′-Deoxy guanosine-5′-triphosphate (dGTP) was obtained from Promega, 2′-deoxyuridine-5′-triphosphate (dUTP) was obtained from Roche, tritiated 2′-deoxyuridine-5′-triphosphate tetraammonium salt ([5–³H] dUTP) and tritiated 2′-deoxyguanosine-5′-triphosphate tetraammonium salt ([8-³H] dGTP) were from Moravek Biochemicals, 2′-deoxyguanosine-5′-[α-thio] triphosphate lithium salt (dGTPαS) was from ChemCyte, guanosine-5′-triphosphate was from Sigma-Aldrich, and C18-reversed phase thin layer chromatography (TLC) plates were purchased from Macherey-Nagel. DEPC-treated water was obtained from Quality Biological, Inc. T7 RNA polymerase, T4 polynucleotide kinase, T7 DNA ligase and restriction enzymes were obtained from New England Biolabs. Pfu Ultra DNA polymerase was obtained from Agilent Technologies.

Seamon K.J., Sun Z., Shlyakhtenko L.S., Lyubchenko Y.L, & Stivers J.T. (2015). SAMHD1 is a single-stranded nucleic acid binding protein with no active site-associated nuclease activity. Nucleic Acids Research, 43(13), 6486-6499.

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Alpaca PBMC RNA Isolation and cDNA Synthesis

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Alpacas from a local breeder were housed at a large animal facility maintained by Johns Hopkins University. PBMC obtained from Ficoll Hypaque centrifugation of peripheral blood was stored in 10⁶ cells/mL aliquots in RNAlater (Ambion, Norwalk, CT) at −80°C. Thawed cells were resuspended in 1 mL of TRIzol Reagent (invitrogen). RNA was extracted in chloroform, precipitated with isopropanol, washed in ethanol, dissolved in DEPC treated water (Quality Biological, Gaithersburg, MD) and stored at −80°C. First-strand cDNA synthesis was performed using SuperScriptIII first strand cDNA synthesis reverse transcription kit (Invitrogen, Grand Island, NY) according to the manufacturer’s protocol. cDNA was stored at −20°C.

Guedon J.T., Luo K., Zhang H, & Markham R.B. (2015). Monoclonal And Single Domain Antibodies Targeting β-Integrin Subunits Block Sexual Transmission of HIV-1 in in vitro and in vivo Model Systems. Journal of acquired immune deficiency syndromes (1999), 69(3), 278-285.

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Surfactant-Based Biomolecule Detection Protocol

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Surfactant poly(ethylene glycol) di-(krytox-FSH amide) was purchased from RAN Biotechnologies (MA, USA). Streptavidin coated magnetic beads (Dynabeads, diameter 1 μm), streptavidin-beta-galactosidase (SβG), resorufin beta-β-galactopyranoside (RGP), 2 M KCl, 1 M MgCl₂, and ultraPure DNase/RNase-free distilled water were all purchased from Life Technologies. Purified synthetic target oligos, capture oligos and detection oligos (DNAs and RNAs) were custom manufactured by Integrated DNA Technologies. Bovine serum albumin (BSA) was purchased from Thermo Scientific. Sodium azide was from Sigma-Aldrich. 0.5 M EDTA (pH 8.0) was purchased from Corning Cellgro. Molecular biology grade Tween-20 (10%) was from Bio-Rad Laboratories. DEPC treated water and 1 M Tris-HCl (pH 8.0) was obtained from Quality Biological. Human total RNA was obtained from Promega. RNA extraction kit (miRNeasy Mini Kit) was from Qiagen.

Guan W., Chen L., Rane T.D, & Wang T.H. (2015). Droplet Digital Enzyme-Linked Oligonucleotide Hybridization Assay for Absolute RNA Quantification. Scientific Reports, 5, 13795.

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