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Dynamic array 96

Manufactured by Standard BioTools
Sourced in Germany

The Dynamic Array 96.96 is a microfluidic integrated circuit that enables high-throughput gene expression analysis. It facilitates the simultaneous measurement of up to 9,216 individual real-time PCR reactions in a single experiment.

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2 protocols using dynamic array 96

1

MicroRNA Profiling in Clinical Samples

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After MicroRNAs were isolated from serum, saliva and tissue by MicroRNA isolation kit (Roche Diagnostics, GmbH, Mannheim, Germany) according to manufacturer's instructions; RNA samples converted to cDNA by using miScript II RT Kit (Qiagen). cDNA samples are preamplified by using miScript Microfluidics PreAMP Kit (Qiagen). qRT-PCR analysis performed by using miScript miRNA Assays (Qiagen) with Dynamic Array 96.96 (Fluidigm) on BioMark System (Fluidigm). Basically 95 types of microRNAs were studied.
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2

Plasma miRNA Isolation and Profiling

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RNAs were isolated from plasma samples by using High Pure microRNA Isolation Kit (Roche Life Science, Mannheim, Germany). RNA samples were then converted to cDNA by using miScript II RT Kit (Qiagen, Hilden, Germany). cDNA samples were pre-amplifi ed by using miScript Microfl uidics PreAMP Kit (Qiagen, Hilden, Germany). qRT-PCR analysis was performed using miScript microRNA Assays (Qiagen, Hilden, Germany) with Dynamic Array 96.96 (Fluidigm, South San Francisco, CA, USA) on BioMark System (Fluidigm, South San Francisco, CA, USA).
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