Kds 200

Manufactured by KD Scientific

Sourced in United States

The KDS 200 is a precision syringe pump designed for accurate fluid delivery. It features a compact design and user-friendly interface. The pump is capable of operating in a wide range of flow rates and can accommodate different syringe sizes.

Automatically generated - may contain errors

Lab products found in correlation

41 protocols using kds 200

Burst and Compliance Testing of Vascular Grafts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Burst pressure and compliance testing was performed in accordance with ANSI/AAMI/ISO 7198 and as described previously.²⁴ Briefly, a nonporous latex tube lining was first inserted into 40 mm long grafts. Static compliance was determined by pumping water through a syringe pump (KDS200, KDScientific) at a rate of 4 mL/min to subject each graft to a pressure ramp (0–150 mmHg). Intraluminal pressure was monitored using an in-line digital pressure gauge (MG1-5-A-9V-R MediaGauge, SSI Technologies, Inc) and graft outer diameter was measured with a He-Ne laser micrometer (Lasermike). Compliance (C) was calculated from the recorded pressure, P, and inner diameter, D, according to the following equation:

C = Δ D / (D_{0} • Δ P) = (D_{120} - D_{80}) / (D_{80} • 40)

Inner diameter was calculated by subtracting the two times the wall thickness from the measured external diameter, assuming incompressibility of the graft wall. Burst pressure was determined by pumping deionized water into each latex lined graft at 100 mL/min using a syringe pump (KDS200, KDScientific). The ends of each graft were firmly secured and sealed to prevent leakage. Pressure was measured using a high pressure gauge (0 to 60 psi pressure range, NoShok) connected downstream of the graft. Burst pressure was recorded as the maximum pressure prior to construct failure.

Nezarati R.M., Eifert M.B., Dempsey D.K, & Cosgriff-Hernandez E. (2014). Electrospun Vascular Grafts with Improved Compliance Matching to Native Vessels. Journal of biomedical materials research. Part B, Applied biomaterials, 103(2), 313-323.

+ Open protocol

+ Expand

Chemogenetic and Neurotrophic Manipulations of the Basolateral Amygdala

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rats anesthetized with 5% chloral hydrate were implanted bilaterally with guide cannulas to the BLA or IL. The coordinates were as follows: BLA: anteroposterior (AP), −2.97 mm; lateral (L), ±5.1 mm; dorsoventral (V), −7.0 mm; IL: anteroposterior (AP), +3.14 mm; lateral (L), ±0.3 mm; dorsoventral (V), −4.0 mm. To prevent clogging, a stylus was placed into the cannula. Bilateral infusion cannulas (28 gauges) were inserted 7 days later, extending 1.5 mm beyond the tip of the guide cannulas. The injection cannula was linked via PE20 tubing to a 10-μl Hamilton microsyringe motored by a microinjection pump (KDS 200, KD Scientific, Holliston, MA, USA). Infusions were administered with a volume of 0.5 μl over the course of 2 min, and an additional 2 min was allowed for diffusion before the infusion cannulas were removed. BDNF (250 ng/μl, 1 μl/lateral) was administered into the BLA, and AAV5-CamkIIα-EGFP (6.72 × 10¹³ vp/ml, ViGene Bioscience, Jinan, China, 1.5 μl/lateral), AAV5-CamkIIα-CC1-EGFP (5.84 × 10¹³ vp/ml, ViGene Bioscience, 1.5 μl/lateral), and AAV5-CamkIIα-TrkB-mCherry (1.64 × 10¹⁴ vp/ml, ViGene Bioscience, 1 μl/lateral) were injected into the BLA 24 h after acquisition of the auditory fear memory.

Li Y., Wang D., Li Y., Chu H., Zhang L., Hou M., Jiang X., Chen Z., Su B, & Sun T. (2017). Pre-synaptic TrkB in basolateral amygdala neurons mediates BDNF signaling transmission in memory extinction. Cell Death & Disease, 8(7), e2959-.

+ Open protocol

+ Expand

Fabrication of SBS Nanofiber Membrane

Check if the same lab product or an alternative is used in the 5 most similar protocols

A purpose-made electrospinning setup was employed for making SBS nanofibrous membrane26 . During electrospinning, the SBS solution was charged with high voltage using a power supply (ES30P, Gamma High Voltage Research), and a metal plate was used as collector. The flow rate of the polymer solution was controlled by a digital syringe pump (KDS-200, KD Scientific). The applied voltage, flow rate of the SBS solution, and spinning distance were controlled at 20.0 kV, 3 ml/h and 17 cm, respectively.

Zhou H., Wang H., Niu H, & Lin T. (2015). Electrospun Fibrous Membranes with Super-large-strain Electric Superhydrophobicity. Scientific Reports, 5, 15863.

+ Open protocol

+ Expand

Photoacoustic detection of gold-based nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

200 μL blood aliquots were spiked with 5 μL of GBNs solution in PBS to achieve a final concentration of GBNs in the range 50 ng/mL to 25 μg/mL. Blood samples were pulled through a square quartz capillary (100 μm i.d., Polymicro Tech., Molex, Phoenix AZ) by a syringe pump (KDS 200, withdraw mode, KD Scientific, Holliston, MA) at a rate of 1 mL/h, Figure 2C. PA signals were recorded for ~ 2 min for each sample (16 μL tested sample volume). Transient PA signals exceeding detection threshold set up for control blood were counted within 10 s long intervals for each sample (~2 μL blood volume per interval) and used to calculate the calibration graph. A tiny sample of GBN spiked blood was diluted with PBS and placed into a 35 mm round dish for PA microscopy analysis.

Nedosekin D.A., Nolan J., Cai C., Bourdo S.E., Nima Z., Biris A.S, & Zharov V.P. (2017). In vivo Noninvasive Analysis of Graphene Nanomaterials Pharmacokinetics in Mice Using Photoacoustic Detection. Journal of applied toxicology : JAT, 37(11), 1297-1304.

+ Open protocol

+ Expand

Microfluidic Droplet Generation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Liquids were filled in glass syringes (1000 series, Hamilton Company, USA) and supplied to the microfluidic device using syringe pumps (KDS200, KD Scientific, USA). An optical microscope (BX-51, Olympus, Japan) equipped with a high-speed video camera (Fastcam Mini AX50, Photron, Japan) was used for the observation of droplet generation. Software ImageJ (National Institutes of Health, NY, USA) was used to measure the diameters of droplets.

Xu S, & Nisisako T. (2020). Polymer Capsules with Tunable Shell Thickness Synthesized via Janus-to-core shell Transition of Biphasic Droplets Produced in a Microfluidic Flow-Focusing Device. Scientific Reports, 10, 4549.

+ Open protocol

+ Expand

Heparin-Rosuvastatin Coaxial Nanofiber Stent

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nanofiber was fabricated, as previously described [65 (link)]. P(LLA-CL) was dissolved in 10 mL of hexafluoroisopropanol to prepare a 120 mg/mL shell solution, and the core solution was prepared with 15% heparin and 20 µM rosuvastatin solution (Sigma-Aldrich, Merck, Germany). The volumetric ratios of heparin:rosuvastatin were 450:50 [450:50 (µL)], 425:75 [425:75 (µL)], and 400:100 [400:100 (µL)], and the control group used PBS to replace heparin and rosuvastatin. The nanofiber mats were labeled as control, Rosu 50, Rosu 75, and Rosu 100 according to the different rosuvastatin volumetric ratios. An Apollo bare metal stent made of 316L stainless steel (MicroPort Co., Ltd., Shanghai, China) was placed 10 cm to 25 cm away from the tip of the syringe pump (KDS 200; KD Scientific, Holliston, MA, USA). The core and shell solutions were then used to fabricate the coaxial nanofiber-covered stents by collecting nanofibers with a rotating Apollo bare metal stent (600 rpm, F = 2.3 mm, and l = 7 mm) at room temperature. The structure of the covered stent was observed using a transmission electron microscope (Hitachi, Tokyo, Japan).

Liu Y., Liu P., Song Y., Li S., Shi Y., Quan K., Yu G., Li P., An Q, & Zhu W. (2021). A heparin–rosuvastatin-loaded P(LLA-CL) nanofiber-covered stent inhibits inflammatory smooth-muscle cell viability to reduce in-stent stenosis and thrombosis. Journal of Nanobiotechnology, 19, 123.

+ Open protocol

+ Expand

Electrochemical Glucose Biosensor Development

Check if the same lab product or an alternative is used in the 5 most similar protocols

PCL (average molecular mass: 80,000 g/mol), chloroform (purity > 99%), ethanol (99.5%), glucose oxidase (from Aspergillus niger, 145,200 units/g solid), horseradish peroxidase (297,000 units/g solid, suitable for manufacturing of diagnostic kits and reagents), trehalose dihydrate (from Saccharomyces cerevisiae, > 99%,) and 3,3’,5’,5’-tetramethylbenzidine (purity > 99%) (TMB) were purchased from Merck KgaA, Corp. (Tokyo, Japan). D (+)-Glucose was purchased from Hayashi Pure Chemical Ind., Ltd. (Osaka, Japan). A phosphate buffer solution was purchased from Nacalai Tesque, Inc. (Kyoto, Japan). A high-voltage power supply (model HJPQ 30P1, Matsusada Precision, Japan) was used. The syringe pump was purchased from KD Scientific, Inc. (Holliston, MA) (model KDS 200).
A syringe of 5 mL with a metallic blunt tip of 18 gauge was used. Air plasma treatment was performed using a plasma generator (Sakigake YHS-R, Japan), and the water contact angle was measured using a solid-liquid interface analyser DropMaster 300 (Kyowa Interface Science Co., Ltd., Japan).

Xu C., Bonfante G., Park J., Salles V, & Kim B. (2023). Fabrication of an electrospun polycaprolactone substrate for colorimetric bioassays. Biomedical Microdevices, 25(3), 32.

+ Open protocol

+ Expand

Blood Clot Flow Rate Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

A KD Scientific (KDS200, USA) syringe pump was used for the pumping the blood clot and clot in the LDPE tube. On the syringe pump, at an ID (d1) setting of 8.66 mm (3 ml, BD plastic syringe), the maximum volumetric flow rate, V1, delivered by syringe pump is 7 ml/min. This calculates to a linear displacement of 0.2 cm/sec [linear displacement = V1/π(d1/2) 2 ]. With these same settings, a 60 ml Luer-Lock syringe, which has an ID of 26.72 mm (d2), was mounted on the syringe pump. Hence, the pump's actual volumetric flow rate computes to be 1.11 cm 3 /sec [V2 = linear displacement × π(d2/2) 2 ]. As the ID of the LDPE tube was 1.15 mm (d3); the linear flow rate of blood and blood clot in the tube is computed to be 107 cm/sec [V2/π(d3/2) 2 ]. Similarly, for a setting of 2 ml/min (V1) and 4 ml/min (V1) at a set ID of 8.66 mm (d1) on the syringe pump and using a 60 ml syringe on the pump instead, the linear flow rate of blood and blood clot in the LDPE tube is computed to be 31 cm/sec and 61 cm/sec.

Das D., Sivasubramanian K., Rajendran P, & Pramanik M. (2021). Label-free high frame rate imaging of circulating blood clots using a dual modal ultrasound and photoacoustic system. Journal of biophotonics, 14(3).

+ Open protocol

+ Expand

Electrospinning of Polylactic Acid (PLA) Fibers

Check if the same lab product or an alternative is used in the 5 most similar protocols

PLA (Mw = 100,000, NatureWorks LLC, Minnetonka, MN) was dissolved in a mixed solvent of dichloromethane and N,N-dimethylacetamide (8/2 w/w) by stirring for 48 h at room temperature to obtain homogeneous 10 wt% solution. The PLA solution was loaded in a syringe, which was driven by a syringe pump (KDS200, KD Scientific Inc., Holliston, MA) at a feeding rate of 2.0 mL/h. A Teflon tube was used to connect the syringe and a 21G needle (inner diameter of 0.5 mm), which was set up horizontally. A voltage regulated DC power supply (NNC-30kV-2mA portable type, NanoNC, South Korea) was used to apply a voltage varying from 15 to 20 kV to the PLA solution to generate the polymer jet. The electrospun fibers were deposited in the form of a web on collectors covered by aluminum foil. For collecting random fibers, the tip-to-collector distance (TCD) was set to 16 cm on a flat plate as a collector. For collecting aligned fibers, a rotating drum (~2400 rpm) was placed at 12 cm from the tangent of the drum to the needle tip. To account for different collector sizes and effect of gravity, the TCD distances were optimized for the formation of electrospun PLA fibers of consistent and of comparable diameter. An overview of the systems used to generate random and aligned electrospun scaffolds is shown in Figures 1A and 1B.

Baek J., Chen X., Sovani S., Jin S., Grogan S.P, & D’Lima D.D. (2015). Meniscus Tissue Engineering Using a Novel Combination of Electrospun Scaffolds and Human Meniscus Cells Embedded within an Extracellular Matrix Hydrogel. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 33(4), 572-583.

+ Open protocol

+ Expand

Electrochemical Sensing of Dopamine

Cited 7 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

CF arrays were tested in vitro using a flow cell apparatus to extract dopamine sensitivity measurements and CV standards (ESI^†, Fig. S2). Solutions used in the flow cell were phosphate buffered saline (PBS) or artificial cerebrospinal fluid (aCSF) buffer and dopamine hydrochloride (Sigma-Aldrich, H8502) as dissolved to a concentration of 0.25, 0.5, and 1 μM in the buffer. A syringe pump (KD Scientific, KDS 200) was used to generate a fixed flow rate (~ 1–3 ml/min) to the outlet of a flexible tube (1/8 inch inner diameter) where the sensors were positioned. A sample injection valve (Valco, Model 22Z) was used to switch the sample flowing across the sensors between PBS and dopamine solutions with an electronic actuator. As the CF sensing interfaces comprise identical surfaces to traditional CFM sensors (same CF material), observed dopamine sensitivity (2–50 nA/μM) (ESI^†, Fig. S2) is within range of prior work ^{3 (link),4 (link),9 (link),15 (link),17 (link),18 (link),32 (link),19 (link)–21 (link),31 (link)}.

Schwerdt H.N., Kim M., Amemori S., Homma D., Yoshida T., Shimazu H., Yerramreddy H., Karasan E., Langer R., Graybiel A.M, & Cima M.J. (2017). Subcellular Probes for Neurochemical Recording from Multiple Brain Sites. Lab on a chip, 17(6), 1104-1115.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!