Tight junction antibody sampler kit

pLKO.1 lentiviral vectors harboring short hairpin RNAs (shRNAs) targeting Src or green fluorescent protein (GFP) were obtained from Open Biosystems (Huntsville, AL). Saracatinib and dasatinib were purchased from Selleckchem (Houston, TX). For Western blot, antibodies that recognize p-Src (Tyr416) and Src were purchased from Cell Signaling Technology (Beverly, MA). β-Actin antibody was purchased from Sigma-Aldrich (St Louis, MO). Epithelial-Mesenchymal Transition (EMT) Antibody Sampler Kit (#9782) and Tight Junction Antibody Sampler Kit (#8683) were purchased from Cell Signaling Technology (Beverly, MA).

Phorbol-12-myristate-13-acetate (PMA), gelatin, Triton X-100, Coomassie brilliant blue R-250 was purchased from Sigma-Aldrich, MMP-9 inhibitor (SB-3CT) and NF-κB inhibitor (sc-75741) were purchased from Selleck. Recombinant human MMP-9 protein and Recombinant mice MMP-9 protein were purchased from R&D systems. Recombinant human pro-MMP-9 protein were purchased from ProSpec. Commercialized DENV2-NS1 protein were purchased from Native Antigen. Trizol reagent was purchased from Ambion. Lipofectamine 2000 reagent was purchased from Invitrogen. Human MMP-9 ELISA kit was purchased from BD Biosciences. Membrane maker Dil (D8700) was purchased from Solarbio. NF-κB Pathway Sampler Kit (#9936T), Tight Junction Antibody Sampler Kit (#8683T), Cadherin-Catenin Antibody Sampler Kit (#9961T), and Antibodies against TIMP-1 (8946S) were purchased from Cell Signaling Technology. Antibody against DENV-NS3 (GTX124252) were purchased from Genetex. Antibodies against DENV2-NS1 were purchased from Arigo biolaboratories (SQab1501). Antibodies against Flag (F3165) and HA (H6908) were purchased from Sigma. Anti-β-actin antibody (66009) were purchased from Proteintech. Rabbit IgG and Mouse IgG were purchased from Invitrogen. Anti-Mouse IgG Dylight 649, Anti-Mouse IgG Dylight 488, Anti-Rabbit IgG Dylight 649, and Anti-Rabbit IgG FITC were purchased from Abbkine.

CYT997 was purchased from Selleckchem (Houston, TX). Adenosine triphosphate (ATP) and β‐actin antibody were purchased from Sigma‐Aldrich (St Louis, MO). Antibodies that recognize HSP60, DRP1, PTPMT1, Cytochrome C, IF2, ATPAF1, IF1, and COXIV were obtained from Santa Cruz Biotechnology (Dallas, TX). EMT Antibody Sampler Kit and Tight Junction Antibody Sampler Kit were purchased from Cell Signaling Technology (Beverly, MA). Intracellular ATP levels were determined by ENLITEN^® ATP Assay System (Promega, Madison, MI). Western blot and wound healing assays were carried out as previously described.6, 10, 13, 14, 15