Wm983b

The A375 and Malme-3M melanoma cell lines lines used in this study were purchased from the ATCC. The WM983B melanoma cell line was a gift from L. Larue (Institut Curie, France). The A375 and WM983B cell lines were maintained at 37 °C and 5% CO₂ in a humidified atmosphere and grown in Dulbecco’s modified Eagle’s medium (DMEM) growth media supplemented with 10% fetal bovine serum (FBS) and 2 mM glutamine (Gibco). The Malme-3M cells were grown in Iscove’s modified Dulbecco’s medium supplemented with 20% FBS. The PC9 lung cancer cell line was grown in RPMI1640 supplemented with 10% FBS. All cell lines were regularly controlled to be mycoplasma-free by using a PCR-based test (Biovalley). BRAF inhibitor (PLX4032, #S1267), MEKi (Cobimetinib, #S8041), EGFR inhibitor (Erlotinib, #S1023), KDM6B inhibitor (GSK-J1, #S7581) and CBP/p300 inhibitor (SGC-CBP30, #S7256) were purchased from Selleckchem (Euromedex, France). Silvestrol was purchased from MedChem Tronica (# HY-13251, Sweden). Pateamine A was provided by S. Apcher (Gustave Roussy Institute, France). Hippuristanol was provided by J. Tanaka (University of the Ryukyus, Japan). 4E1RCat (#SML0197) and cycloheximide (CHX, #C104450) was purchased from Sigma. All the chemicals were dissolved in dimethylsulfoxide (DMSO) for in vitro studies. The panel of small-molecule chemical library was obtained from L. Désaubry (Strasbourg, France).

Cells were grown at 37 °C and 10% CO₂ in DMEM (A375M2, A375P, B16F10, 4599, HEK293T and HaCaT) or RPMI (WM1361, WM983B and WM983A) supplemented with 10% FBS and 1% penicillin/streptomycin (all from Gibco). A375M2 and A375P cells were from Prof. Richard Hynes (HHMI, MIT, USA), WM1361 cells were from Prof. Richard Marais (Cancer Research UK Manchester Institute, UK), WM983B and WM983A were purchased from Coriell Institute, B16F10 cells were from Dr. Hector Peinado (CNIO, Spain), 4599 cells were from Dr. Amine Sadok (Institute of Cancer Research, UK) and Prof. Richard Marais, HEK293T cells were from Dr. Jeremy Carlton (The Francis Crick Institute, UK), and HaCaT cells were from Dr. Ester Martin-Villar (IIBM, UAM, Spain). A375M2, A375P, WM983B, WM983A and 4599 were authenticated using short tandem repeat DNA profiling. All cell lines were routinely tested for mycoplasma contamination. All cell lines were kept in culture for a maximum of three to four passages and cell phenotypes were verified routinely.