Lambda 850 uv vis spectrophotometer

Manufactured by PerkinElmer

Sourced in United States

The LAMBDA 850+ UV/Vis Spectrophotometer is a high-performance instrument designed for a wide range of analytical applications. It features a dual-beam optical system and a wide wavelength range, enabling accurate and precise measurements of absorbance, transmittance, and reflectance properties of various samples.

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Lab products found in correlation

Carbon coated copper grid, by Ted Pella (1 mentions) Nanosight system, by Malvern Panalytical (1 mentions) Jem 2100f, by JEOL (1 mentions) Zetasizer, by Malvern Panalytical (1 mentions) Ifs 66v s, by Bruker (1 mentions) Jsm 7900f, by JEOL (1 mentions) Syringe filter, by Merck Group (1 mentions) Quartz cuvette, by PerkinElmer (1 mentions)

10 protocols using lambda 850 uv vis spectrophotometer

In Vitro Hydrolytic Biodegradation and Drug Release

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For the in vitro hydrolytic biodegradation
drug release experiments, five sets of uncoated and 0.1, 1.0, 5.0,
and 10 mg/mL drug-coated fibers, each about 70 cm length, were accurately
weighed and immersed in a screw-top glass bottle containing 10 mL
of the phosphate buffer saline (PBS) solution (pH = 7.40 ± 0.2).
Then, all bottles were immediately placed in an incubator that was
thermostatically controlled at 37.0 ± 1.0 °C. At a certain
period of time, the fiber samples were filtered out and dried under
vacuum at room temperature to constant weight. After that, drug release
behavior of sutures during in vitro degradation was
measured.
The adsorption intensity of the LVFX drug was measured
by a UV spectrophotometer (PerkinElmer LAMBDA 850+ UV/vis spectrophotometer)
at 298 nm using 0.1 M HCl solution as a blank control. The calibration
curve of the LVFX drug (see Supporting Information page S3) was obtained from the plots between the drug concentration
(mg/mL) and absorbance (AU); therefore, the amount of LVFX in the
release medium can be determined by this standard curve and the cumulative
release rate was then calculated according to eq 1 as follows^{79 (link)} where Q_t is the cumulative release rate of LVFX at time t, and m_t and m_i are the amount of released LVFX at time t and initial time, respectively.

Sriyai M., Tasati J., Molloy R., Meepowpan P., Somsunan R., Worajittiphon P., Daranarong D., Meerak J, & Punyodom W. (2021). Development of an Antimicrobial-Coated Absorbable Monofilament Suture from a Medical-Grade Poly(l-lactide-co-ε-caprolactone) Copolymer. ACS Omega, 6(43), 28788-28803.

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Characterization of PEG-coated Gold Nanoparticles

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The XRD pattern of the PEG-AuNP samples was recorded by a Jordan Valley’s D₁ (Jordan Valley Semiconductors Ltd) equipped with a Cu Kα (λ = 1.5406 A°) radiation filter. The obtained PEG-AuNP XRD patterns were then compared with JCPDS cards (JCPDS # 04–0784) present in the XRD software. The chemical structure and functional groups of the PEG-AuNPs were analysed by Fourier transform infrared spectroscopy (FT-IR, Bruker Optics IFS 66 V/S, Germany). The spectrum was measured in transmission mode ranging from 4000–600 cm⁻¹ wave numbers by KBr pellet method. UV–visible spectroscopy was performed with a double-beam Lambda 850 UV/VIS spectrophotometer (Perkin-Elmer Inc., USA). Conjugated and unconjugated samples were analysed in the 400–900 nm spectral range. All spectra were recorded using a 0.0125 mg/mL solution of the PEG-AuNPs. The assembly and morphology of PEG-AuNPs was studied with TEM (JEOL, JEM 2100F). TEM samples were prepared onto carbon-coated copper grids (Ted Pella, USA) and were dried at room temperature for microscopic observation. Electrophoretic mode of zetasizer (Malvern, UK) was used to measure the zeta potential (ζ) on PEG-AuNPs surface. The PEG-AuNPs hydrodynamic diameters were measured by using nanoparticle tracking analysis (NTA). NTA was carried out at room temperature by using a NanoSight system (Malvern, UK).

Farooq M.U., Novosad V., Rozhkova E.A., Wali H., Ali A., Fateh A.A., Neogi P.B., Neogi A, & Wang Z. (2018). RETRACTED ARTICLE: Gold Nanoparticles-enabled Efficient Dual Delivery of Anticancer Therapeutics to HeLa Cells. Scientific Reports, 8, 2907.

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Spectroscopic Characterization of Drug Formulations

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A known concentration of the drug (i.e., 200 μg/mL) was dissolved in DW (for drug loading determination) or phosphate buffer saline (PBS) with a pH 7.4 (for release study) and subjected to UV/Vis scanning in the LAMBDA 850+ UV/Vis Spectrophotometer (PerkinElmer, Waltham, MA, USA) from 800 to 200 nm to obtain the absorption maximum (λ max) of the drug. After determining the drug λ max, the drug concentrations, ranging from 12.5 to 0.2 μg/mL, were measured at 340 nm (λ max), and standard curves were plotted against each concentration point’s absorbance.

Aburayan W.S., Booq R.Y., BinSaleh N.S., Alfassam H.A., Bakr A.A., Bukhary H.A., Alyamani E.J, & Tawfik E.A. (2020). The Delivery of the Novel Drug ‘Halicin’ Using Electrospun Fibers for the Treatment of Pressure Ulcer against Pathogenic Bacteria. Pharmaceutics, 12(12), 1189.

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Synthesis and Characterization of Aromatic Compounds

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All chemicals used were purchased from Sigma Aldrich and used without further purification, unless otherwise noted. Benzaldehyde and pyrrole were purified by distillation once per week, or whenever discoloration appeared. Automated sample grinding was done using a Retsch Mixer Mill MM200. Electronic spectra were recorded on a Perkin Elmer Lambda 850 UV-Vis spectrophotometer, measured from 200-800 nm at 1nm intervals. The samples were placed in quartz cuvettes with 1-cm path length. ¹H NMR spectra were recorded on a Varian 300 MHz nuclear magnetic resonance spectrometer. The MALDI-TOF mass spectrum was collected on an ABI 4700 Proteomics Analyser.

Shy H., Mackin P., Orvieto A.S., Gharbharan D., Peterson G.R., Bampos N, & Hamilton T.D. (2014). Two-step Mechanochemical Synthesis of Porphyrins. Faraday discussions, 170, 59-69.

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Halicin Drug Release Kinetics

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To determine the drug release, a defined weight of approximately 4 mg of the drug-loaded fibers was placed into 25 mL PBS (pH 7.4). The release study was carried out at 37 °C and 50 rpm in a thermostatically shaking incubator. Samples of 3 mL were withdrawn from the buffer solution after 5, 10, 15, 30, 60, 120, and 180 min, and equivalent volumes of pre-warmed fresh buffer were replaced to maintain sink condition. The amount of drug in the release buffer was determined by LAMBDA 850+ UV/Vis Spectrophotometer (PerkinElmer, Waltham, MA, USA) at 340 nm. The cumulative release percentages were measured as a function of time and were calculated based on the following equation:

Cumulative amount of release (%) = \frac{Ct}{C \infty} \times 100

where Ct is the amount of halicin released at time t and C∞ refers to the total amount of drug-loaded into the fibers. The findings represent the mean (±SD) of at least three replicates.

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AMT-Release from Clay Mineral Samples

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The AMT-release tests were conducted using an RCZ-8M dissolution tester (Tianjin TDTF Technology Co. Ltd., China) following a paddle method. Approximately 0.5 g of AMT-loaded clay mineral sample was sealed in a dialysis bag (molecular mass cutoff: 3500 Da), and then soaked in 500 mL of distilled water at room temperature with a rotation speed of 100 rpm. At suitable intervals, 5 mL of the dissolution medium was withdrawn, and an equivalent volume of fresh medium was added. The AMT content was determined at 239 nm using a PerkinElmer LAMBDA 850 UV/Vis spectrophotometer. Each dissolution test was performed in triplicate. After the achievement of 100% release of AMT, the clay mineral samples were collected for thermal analysis to calculate the amount of the intercalated AMT.

Tan D., Yuan P., Annabi-Bergaya F., Liu D, & He H. (2015). Methoxy-modified kaolinite as a novel carrier for high-capacity loading and controlled-release of the herbicide amitrole. Scientific Reports, 5, 8870.

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Quantitative Determination of Ammonia

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To test for the presence of ammonia in the products, a 5 mL sample of the gaseous product was extracted with a gas-tight syringe at ambient pressure and slowly bubbled into 10 mL of miliQ water. Calibration standards for ammonia were prepared by bubbling different volumes of pure ammonia gas into 10 mL of miliQ water. The indophenol reagent was prepared according to a previously reported method^{69 (link)}. After adding the indophenol reagent to the sample or standard, the solution was allowed to stand in the dark for 1 h before measuring the absorbance at 651 nm using a PerkinElmer Lambda 850 + UV-Vis spectrophotometer. The absorbance value of the characteristic maximum peak of the indophenol blue complex was recorded and compared with the calibration standards.

Wu X., Lee W.T., Turnell-Ritson R.C., Delannoi P.C., Lin K.H, & Dyson P.J. (2023). Controlling the selectivity of the hydrogenolysis of polyamides catalysed by ceria-supported metal nanoparticles. Nature Communications, 14, 6524.

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UV-vis Spectrophotometric Analysis

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UV–vis absorption spectra was measured using Lambda 850 UV/vis spectrophotometer (Perkin-Elmer Life and Analytical Sciences, CT, USA) in the range of 300–600 nm operating at a resolution of 1 nm, in the quartz cuvette of 1 cm path length. The BD water was used as a reference material for background correction.

Khan M.F., Ansari A.H., Hameedullah M., Ahmad E., Husain F.M., Zia Q., Baig U., Zaheer M.R., Alam M.M., Khan A.M., AlOthman Z.A., Ahmad I., Ashraf G.M, & Aliev G. (2016). Sol-gel synthesis of thorn-like ZnO nanoparticles endorsing mechanical stirring effect and their antimicrobial activities: Potential role as nano-antibiotics. Scientific Reports, 6, 27689.

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Structural and Optical Characterization of Te

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Powder X-ray diffraction patterns of Te samples were collected at Rigaku SmartLab SE diffractometer equipped with a Cu K_α source (λ = 1.5418 Å). The polished surfaces of samples were observed by scanning electron microscopy (JSM-7900F; JEOL) equipped with energy-dispersive X-ray spectroscopy (EDS). (Scanning) transmission electron microscopy (TEM/STEM) observations were carried out in an FEI Themis Z. STEM foils were prepared by a standard procedure including cutting, grinding, dimpling, polishing, and Ar ion-milling (Gatan PIPS Model691). Optical absorption spectra were measured at 300 K using a Bruker Optik GmbH INVENIO R FTIR spectrophotometer (k = 4000−400 cm⁻¹) equipped with an integrated sphere and a Perkin-Elmer Lambda 850 + UV–vis spectrophotometer (λ = 800−250 nm), respectively. We also performed Ultraviolet photoelectron spectroscopy (UPS) analyses using PHI 5000 VersaProbe III with a He Iα source (21.22 eV) and an applied negative bias of 9 V.

An D., Zhang S., Zhai X., Yang W., Wu R., Zhang H., Fan W., Wang W., Chen S., Cojocaru-Mirédin O., Zhang X.M., Wuttig M, & Yu Y. (2024). Metavalently bonded tellurides: the essence of improved thermoelectric performance in elemental Te. Nature Communications, 15, 3177.

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Batch Adsorption of Antibiotics on PIM-1

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Batch adsorption experiments for the four different antibiotics were conducted with an initial concentration of 200 µM. Five mL solution of the target antibiotics was mixed and stirred (using magnetic bar at a speed of 400 rpm) with PIM-1 (2 mg) at different adsorption times, pH, and temperatures. The solution’s pH was adjust using diluted sodium hydroxide or hydrochloric acid solutions. The samples were filtered with 0.22 µm syringe-filters from Millipore prior to UV-VIS measurements. Unknown concentrations were determined by a comparison against calibration curves. Calibration curves were established from four different concentrations (50, 100, 150, and 200 µM) with excellent linearity in all cases (R² > 0.999). UV-VIS spectra were recorded before and after adsorption using LAMBDA 850 UV/Vis spectrophotometer from PerkinElmer. Quartz cuvettes from PerkinElmer were used for the measurements. The reported adsorption data points in this study represent the average values of three individual experiments with standard deviations not exceeding 5%.

Alnajrani M.N, & Alsager O.A. (2020). Removal of Antibiotics from Water by Polymer of Intrinsic Microporosity: Isotherms, Kinetics, Thermodynamics, and Adsorption Mechanism. Scientific Reports, 10, 794.

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