Gold chloride trihydrate (HAuCl4·3H2O, 99.9%), sodium borohydride (NaBH4, 98%), trisodium citrate (99.5%), gadolinium chloride (GdCl3, 99%), hydrochloric acid (HCl, 36.0–38.0%), nitric acid (HNO3, 65.0–68.0%), silver nitrate (AgNO3, 99.8%), l -ascorbic acid (99.7%), sodium hydroxide (NaOH, 96.0%), cyclohexane (99.7%), tetraethyl orthosilicate and ethanol absolute (C2H6O, 99.7%) were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Triethanolamine (TEA, AR), bromoacetic acid (AR), hexadecyltrimethylammonium bromide (CTAB, 99%) and cetyltrimethylammonium (CTAC, 97%) were bought from Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China). 3-(Trimethoxysilylpropyl)diethylene triamine was purchased from Gelest (Morrisville, US). Fetal bovine serum (FBS, Gibco), phosphate buffered saline (PBS), trypsin–EDTA and Dulbecco's modified Eagle medium (DMEM) were obtained from Beyotime Biotechnology (Nantong, China).
Dulbecco s modified eagle medium dmem
Manufactured by Beyotime
Sourced in China
Dulbecco's modified Eagle medium (DMEM) is a widely used cell culture medium formulation that supports the growth and maintenance of various cell types. It provides essential nutrients, vitamins, amino acids, and other components required for cell proliferation and survival. DMEM is a versatile and commonly used tool in cell biology research and applications.
Automatically generated - may contain errors
Lab products found in correlation
Absolute ethanol, by Sinopharm (1 mentions)
Sodium hydroxide (naoh), by Sinopharm (1 mentions)
Gold chloride trihydrate, by Sinopharm (1 mentions)
Hydrochloric acid (hcl), by Sinopharm (1 mentions)
Trypsin edta, by Beyotime (1 mentions)
Gadolinium chloride, by Sinopharm (1 mentions)
Cyclohexane, by Sinopharm (1 mentions)
Sodium borohydride, by Sinopharm (1 mentions)
Silver nitrate, by Sinopharm (1 mentions)
Trisodium citrate, by Sinopharm (1 mentions)
Nitric acid, by Sinopharm (1 mentions)
L ascorbic acid, by Sinopharm (1 mentions)
Tetraethyl orthosilicate, by Sinopharm (1 mentions)
24 well plate, by NEST Biotechnology (1 mentions)
Propidium iodide, by Beyotime (1 mentions)
Hydrogen tetrachloroaurate 3 tetrahydrate haucl4 3h2o, by Merck Group (1 mentions)
3 protocols using dulbecco s modified eagle medium dmem
1
Synthesis of Multifunctional Nanoparticles
2
HT22 Cell Line Oxygenic Glucose Deprivation
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The mouse hippocampal neuronal HT22 cell line was purchased from a public cell bank (ATCC, Manassas, VA, USA). The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Beyotime Biotechnology) supplemented with 10% fetal bovine serum (HyClone, Ogden, UT, USA) and 1% penicillin/streptomycin (HyClone) in an incubator (Heraeus, Hanau, Germany) at 37 °C in 5% CO2. For OGD, HT22 cells were seeded in 96-well plates at a density of 1 × 105 cells/mL and were cultured in glucose-free DMEM at 37 °C in 0.5% O2, 94.5% N2, and 5% CO2 for 4 h. The cells were then incubated in a maintenance medium for 24 h under normal conditions before subsequent experiments.
HT22 cells in the control group were treated identically except that they were not exposed to OGD. Experimental treatment groups were as follows: (1) the control group (Con), (2) the OGD group (OGD), (3) the OGD + 4-PBA (4 µM) treatment group (4-PBA), (4) the OGD + URB597 (2 µM) treatment group (URB), and (6) the OGD + TG (0.02 µM) treatment group (TG).
HT22 cells in the control group were treated identically except that they were not exposed to OGD. Experimental treatment groups were as follows: (1) the control group (Con), (2) the OGD group (OGD), (3) the OGD + 4-PBA (4 µM) treatment group (4-PBA), (4) the OGD + URB597 (2 µM) treatment group (URB), and (6) the OGD + TG (0.02 µM) treatment group (TG).
3
Poly(butylene succinate) for Drug Delivery
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Poly(buttlene succinate) (PBS, Mn = 30 kDa) was purchased from Dongguan Hongli Plastic Raw Material Co., Ltd. Lauric acid (LA), doxorubicin hydrochloride (DOX), 2methylimidazole (2-MeIM), cetyltrimethylammonium bromide (CTAB), and hydrogen tetra-chloroaurate (III) tetrahydrate (HAuCl4•3H2O) were bought from Sigma Aldrich (Shanghai, China). Fetal bovine serum (FBS), calcein acetoxymethyl ester (calcein-AM), phosphate buffer solution, propidium iodide (PI) and Dulbecco's modified eagle medium (DMEM) were bought from the Beyotime Institute of Biotechnology (China).
Zinc nitrate hexahydrate (Zn(NO3)2•6H2O), sodium borohydride (NaBH4), silver nitrate (AgNO3), hydroquinone, and chloroform were purchased from Guangzhou Chem. Co.
(Guangzhou, China). Methoxy-poly(ethylene glycol) thiol (MPEG-SH, 2000 Da) was purchased from Hua Wei Rui Ke Chemical Co. Ltd. (China). 24-well plates were purchased from NEST Biotechnology (China). Unless otherwise stated, all reagents were used as received.
Zinc nitrate hexahydrate (Zn(NO3)2•6H2O), sodium borohydride (NaBH4), silver nitrate (AgNO3), hydroquinone, and chloroform were purchased from Guangzhou Chem. Co.
(Guangzhou, China). Methoxy-poly(ethylene glycol) thiol (MPEG-SH, 2000 Da) was purchased from Hua Wei Rui Ke Chemical Co. Ltd. (China). 24-well plates were purchased from NEST Biotechnology (China). Unless otherwise stated, all reagents were used as received.
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