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Anti cd3 17a2

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Anti-CD3 (17A2) is a monoclonal antibody that specifically binds to the CD3 complex, which is expressed on the surface of T cells. This antibody is a useful tool for research purposes.

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Lab products found in correlation

Anti cd4 rm4 5, by BD (5 mentions) Canto 2, by BD (2 mentions) Brefeldin a, by Merck Group (2 mentions) Anti cd11b m1 70, by BD (2 mentions) Anti cd62l mel 14, by Thermo Fisher Scientific (2 mentions) Anti il 13 ebio13a, by Thermo Fisher Scientific (2 mentions) Anti cd19 6d5, by BioLegend (2 mentions) Anti cd90.2 anti thy 1 2 53 2 1, by Thermo Fisher Scientific (2 mentions) Anti cd49b dx5, by Thermo Fisher Scientific (2 mentions) Anti cd49b dx5, by BD (1 mentions) Anti cd45 30 f11, by BD (1 mentions) Anti cd25 pc61, by BioLegend (1 mentions) Anti cd8 53 6, by Miltenyi Biotec (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Anti foxp3 fjk 16s, by Thermo Fisher Scientific (1 mentions) Anti cd25 pc61, by BD (1 mentions) Anti cd11c n418, by Thermo Fisher Scientific (1 mentions) Anti cd11b m1 70, by BioLegend (1 mentions) Anti cd11c clone n418, by BioLegend (1 mentions) Attune nxt cytometer, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-CD3 (541 citations) CD3 (17A2; (10 citations) Anti-CD3 (Clone 17A2, (3 citations) Anti-CD3 APC-Cy7 clone 17A2 (2 citations) Anti-mouse CD3-FITC (clone 17A2, (2 citations) PE anti-mouse CD3 molecular complex (17A2) (2 citations) Soluble rat anti-mouse CD3 (clone 17A2; (2 citations)

7 protocols using anti cd3 17a2

1

Comprehensive Tumor Immune Profiling

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Flow cytometry was used to analyze the tumor immune microenvironment. All tumor tissue samples per group were collected. Cells from the tumors were counted and cell surface markers were stained with the following fluorescently conjugated antibodies: anti-CD45 (30F11, BD Biosciences), anti-CD4 (RM4-5, BD Biosciences), anti-CD8 (53-6.7, Miltenyi Biotec), anti-CD3 (17A2, BD Biosciences), antiCD11b (M1/70, Invitrogen), anti-PD-1 (10F.9G2, BioLegend), anti-CD25 (PC61, Biolegend), anti-CD49b (DX5, BD Biosciences), anti-Granzyme b/Cryofix (GB11, Invitrogen), Viability UV Zombie. Flow cytometry data were acquired on the BD LSRFortessa X20 cytometer and FlowJo software (Ashland, OR, USA) was used for analyses.
Gouez M., Rébillard A., Thomas A., Beaumel S., Matera E.L., Gouraud E., Orfila L., Martin B., Pérol O., Chaveroux C., Chirico E.N., Dumontet C., Fervers B, & Pialoux V. (2024). Combined effects of exercise and immuno-chemotherapy treatments on tumor growth in MC38 colorectal cancer-bearing mice. Frontiers in Immunology, 15, 1368550.
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2

Comprehensive Immunophenotyping by Flow Cytometry

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Flow cytometry was performed using fluorochrome-labeled Abs for surface staining anti-CD90.1 (OX-7), anti-CD90.2 (53-2.1), anti-CD4 (RM4.5), anti-CD69 (H12F3), anti-CD127 (A7R34), anti-CD122 (TM-β1), anti-CD103 (B-Ly7), anti-KLRG1 (2F1), anti-CDH1 (DECMA-1), anti-SLAMF6 (13G3-19D), anti-RANKL (IK22/5), anti-CD40 (1C10), anti-CD86 (GL1), anti-MHC-II (M5/114.15.2), anti-CD11c (HL3), anti-NK1.1 (PK136), anti-CD11b (M1/70), and anti-CD3 (17A2) (BD Biosciences, eBioscience, or BioLegend). Intracellular cytokine staining was performed by stimulating cells for 16 h with OVAII peptide-pulsed APC. After 2h, 10 μg/ml Brefeldin A (Sigma) was added and after a further 2 h, the cells were surface stained and fixed for 20 min in 4% paraformaldehyde. Intracellular staining was performed by permeabilizing the cells for 10 min in 0.1% saponin before staining for cytokine by the addition of anti-IFN-γ (XMG1.2), anti-IL-2 (JES-5H4), anti-IL-6 (MP5-20F3), anti-IL-10 (JES-16E3), anti-IL-12 (C17.8), and anti-IL-17 (eBio17B7) fluorescently labeled Abs. Analysis was performed using LSRII or Canto II instruments (BD Biosciences) and FlowJo (Tree Star) analysis software.
Strutt T.M., Dhume K., Finn C.M., Hwang J.H., Castonguay C., Swain S.L, & McKinstry K.K. (2017). IL-15 supports the generation of protective lung-resident memory CD4 T cells. Mucosal immunology, 11(3), 668-680.
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3

Comprehensive Flow Cytometry Immunophenotyping

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The following antibodies were used in flow cytometry with the indicated antibody clones and manufacturers for the mouse experiments: Anti-CD3 (17A2; BD Biosciences), anti-CD19 (6D5; BioLegend), anti-CD11c (clone: N418; Biolegend), anti-CD11b (M1/70; BioLegend), anti-CD49b (DX5; eBioscience), anti-CD25 (PC61; BD Biosciences), anti-CD90.2 (anti-Thy-1.2; 53 2.1; eBioscience), anti-CD11c (N418; eBioscience), anti-ST2 (DIH9; BioLegend), anti-Siglec-F (clone: E50-2440; Biolegend) anti-CD4 (RM4-5; BD Biosciences), anti-IL-13 (ebio13A, eBioscience), anti-Foxp3 (FJK-16S; eBioscience), anti-CD62L(MEL-14; eBioscience), LGR6 (17658-1-AP; Proteintech). Flow cytometric data acquisition was performed on an LSRFortessa flow cytometer (BD Biosciences), and the data analyzed using FlowJo software version 10.3 (Tree Star). The gating strategy is provided in SI Appendix, Fig. S7 for populations analyzed.
Krishnamoorthy N., Walker K.H., Brüggemann T.R., Tavares L.P., Smith E.W., Nijmeh J., Bai Y., Ai X., Cagnina R.E., Duvall M.G., Lehoczky J.A, & Levy B.D. (2023). The Maresin 1–LGR6 axis decreases respiratory syncytial virus-induced lung inflammation. Proceedings of the National Academy of Sciences of the United States of America, 120(2), e2206480120.
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4

Multiparametric Flow Cytometry Analysis of Tumor-Infiltrating Immune Cells

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Anti-CD3 (17A2, cat. 561388), anti-CD4 (RM4-5, cat. 553047), anti-CD44 (IM7, cat. 561859), anti-CD62L (MEL-14, cat. 560516), anti-B220 (RA3-6B2, cat. 553091), anti-Foxp3 (MF23, cat. 562996) and anti-CD23 (B3B4, cat. 561772) were purchased from BD company (Franklin Lakes, NJ, USA). Anti-CD8a (53–6.7, cat. 100734), anti-IgM (RMM-1, cat. 406531) and anti-CD21 (7E9, cat. 123411) were obtained from BioLegend (San Diego, CA, USA). For flow cytometry experiments, total eight axillary LNs were dissected from four each of normal and tumor-bearing mice and pooled separately. Cells were harvested and washed with 1X PBS. Cells were stained with antibodies within 1:100 dilution for 40 min at 4°C with gentle mix, then washed with 1X PBS prior to Attune NxT cytometer (Thermo Fisher Scientific) analysis. For Foxp3 staining, cells were mixed with 1 ml of Fix/Perm solution and incubated at room temperature for 15 min. Cells were washed with 1X PBS and stained with anti-Foxp3 antibody for flow cytometry analysis.
Li Y.L., Chen C.H., Chen J.Y., Lai Y.S., Wang S.C., Jiang S.S, & Hung W.C. (2020). Single-cell analysis reveals immune modulation and metabolic switch in tumor-draining lymph nodes. Oncoimmunology, 9(1), 1830513.
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5

Multiparameter Flow Cytometry Panel

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The following antibodies were used in flow cytometry:
Anti-CD3 (17A2; BD Biosciences), anti-CD19 (6D5 BioLegend), anti-CD11b Pacific Blue (M1/70; BioLegend), anti-CD49b(DX5; eBioscience), anti-CD25 APC (PC61; BD Biosciences) ; anti-CD90.2 (anti-Thy-1.2; 53-2.1; eBioscience); CD11c (N418; eBioscience); and anti-ST2(Biolegend), Anti-CD4 (RM4-5, BD Biosciences), Foxp3(FJK-16S, eBioscience,), anti-CD62L(MEL-14, eBioscience), anti-IL-13(ebio13A, eBioscience), and anti IL-5 (TRFK5, BioLegend).
Krishnamoorthy N., Burkett P.R., Dalli J., Abdulnour R.E., Colas R., Ramon S., Phipps R.P., Petasis N.A., Kuchroo V.K., Serhan C.N, & Levy B.D. (2014). Maresin-1 engages regulatory T cells to limit type 2 innate lymphoid cell activation and promote resolution of lung inflammation. Journal of immunology (Baltimore, Md. : 1950), 194(3), 863-867.
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6

Comprehensive Immunophenotyping by Flow Cytometry

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Flow cytometry was performed using fluorochrome-labeled Abs for surface staining anti-CD90.1 (OX-7), anti-CD90.2 (53-2.1), anti-CD4 (RM4.5), anti-CD69 (H12F3), anti-CD127 (A7R34), anti-CD122 (TM-β1), anti-CD103 (B-Ly7), anti-KLRG1 (2F1), anti-CDH1 (DECMA-1), anti-SLAMF6 (13G3-19D), anti-RANKL (IK22/5), anti-CD40 (1C10), anti-CD86 (GL1), anti-MHC-II (M5/114.15.2), anti-CD11c (HL3), anti-NK1.1 (PK136), anti-CD11b (M1/70), and anti-CD3 (17A2) (BD Biosciences, eBioscience, or BioLegend). Intracellular cytokine staining was performed by stimulating cells for 16 h with OVAII peptide-pulsed APC. After 2h, 10 μg/ml Brefeldin A (Sigma) was added and after a further 2 h, the cells were surface stained and fixed for 20 min in 4% paraformaldehyde. Intracellular staining was performed by permeabilizing the cells for 10 min in 0.1% saponin before staining for cytokine by the addition of anti-IFN-γ (XMG1.2), anti-IL-2 (JES-5H4), anti-IL-6 (MP5-20F3), anti-IL-10 (JES-16E3), anti-IL-12 (C17.8), and anti-IL-17 (eBio17B7) fluorescently labeled Abs. Analysis was performed using LSRII or Canto II instruments (BD Biosciences) and FlowJo (Tree Star) analysis software.
Strutt T.M., Dhume K., Finn C.M., Hwang J.H., Castonguay C., Swain S.L, & McKinstry K.K. (2017). IL-15 supports the generation of protective lung-resident memory CD4 T cells. Mucosal immunology, 11(3), 668-680.
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7

Flow Cytometry for Whole Blood Cell Profiling

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Cell populations in whole blood were identified and quantified by flow cytometry with the following antibodies: anti-CD115 (AF598, eBioscience), anti-Gr-1 (RB6-8C5, Biolegend), anti-CD3 (17A2, BD Bioscience), anti-CD4 (RM4-5, BD Bioscience), CD8a (53-6.7, BD Bioscience), anti-B220 (RA3-6B2, Biolegend). Antibodies were added directly to 100 mL of heparinized whole blood at a 1:200 dilution and incubated at room temperature for 30 minutes. Stained cells were fixed and erythrocytes were lysed simultaneously with 1-step Fix/Lyse solution (Affymetrix eBioscience). AccuCheck Counting beads (Invitrogen) were added to obtain total cell counts. Samples were acquired on a BD LSRFortessa using DIVA software and analyzed by Flowjo (Treestar) software.
Amulic B., Knackstedt S.L., Abu Abed U., Deigendesch N., Harbort C.J., Caffrey B.E., Brinkmann V., Heppner F.L., Hinds P.W, & Zychlinsky A. (2017). Cell-Cycle Proteins Control Production of Neutrophil Extracellular Traps. Developmental cell, 43(4).
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