Log In Sign up
The largest database of trusted experimental protocols

Rabbit anti p53 antibody

Manufactured by Proteintech
Visit Supplier
Sourced in China

Rabbit anti-P53 antibody is a primary antibody that recognizes the p53 protein. P53 is a tumor suppressor protein that plays a crucial role in regulating cell growth and division. This antibody can be used for various applications, such as Western blotting, immunohistochemistry, and immunoprecipitation, to detect and study the p53 protein in different biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti ppar α antibody, by Proteintech (4 mentions) Bca kit, by Thermo Fisher Scientific (3 mentions) Histone simple stain kit, by Nichirei Biosciences (2 mentions) Immobilon, by Merck Group (2 mentions) Anti β actin, by Cell Signaling Technology (2 mentions) Enhanced chemiluminescence, by Merck Group (1 mentions) Image lab analyzer software, by Bio-Rad (1 mentions) Rabbit anti seh antibody, by Abcam (1 mentions) Rabbit anti cox 2 antibody, by Wuhan Servicebio Technology (1 mentions) Chemidoc xr, by Bio-Rad (1 mentions) Ripa buffer, by Solarbio (1 mentions) Protease inhibitor, by Solarbio (1 mentions) Rabbit anti tgf β antibody, by Proteintech (1 mentions) Rabbit anti collagen 1 antibody, by Proteintech (1 mentions) Rabbit anti collagen 3 antibody, by Proteintech (1 mentions) Rabbit anti bcl 2 antibody, by Proteintech (1 mentions) Rabbit anti α sma antibody, by Proteintech (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Bcl 2, by Proteintech (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-p53 (49 citations) P53 antibody (6 citations) Anti-p53 antibody (5 citations) Rabbit anti-p53 (2 citations) Anti-TM (2 citations)

7 protocols using rabbit anti p53 antibody

1

SIRT1-Mediated p53 Acetylation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The SIRT1 assay mixture (45 µl) containing 50 mm Tris‐HCl, pH 9.0, 4 mm MgCl2, 0.2 mm DTT, 1 mm NAD+, 1% DMSO, and 0.5 µg SIRT1 was mixed with 5 µl eluted full‐length Ac‐Lys382‐p53, and the reaction was carried out at 37 °C for 3 h. Western blot analyses were conducted after the reaction. Ac‐Lys382‐p53 and total p53 were detected by using a 1:1000 rabbit anti‐acetyl‐Lys382‐p53 antibody (Abcam) and a 1:1000 rabbit anti‐p53 antibody (Proteintech). The secondary antibody used was 1:5000 IPKine HRP, Mouse Anti‐Rabbit IgG LCS (Abbkine).
Xie L., Li C., Wang C., Wu Z., Wang C., Chen C., Chen X., Zhou D., Zhou Q., Lu P., Ding C., Liu C., Lin J., Zhang X., Yu X, & Yu W. (2024). Aspirin‐Mediated Acetylation of SIRT1 Maintains Intestinal Immune Homeostasis. Advanced Science, 11(19), 2306378.
+ Open protocol
+ Expand
2

Western Blot Analysis of Lung Cell Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues and A549 cells were harvested, lysed in RIPA buffer (Solarbio, Beijing, China) containing protease inhibitor PMSF (Solarbio). Protein concentrations were determined using a BCA kit (Thermo Fisher Scientific, USA). Proteins were separated on 8% or 12% SDS-PAGE Gels. Separated proteins were transferred onto polyvinylidene difluoride (PDVF) membranes, which were blocked with 5% non-fat milk in TBST and incubated with the primary antibodies overnight at 4°C. Subsequently, membranes were incubated with appropriate secondary HRP-linked antibodies. Proteins were visualized by enhanced chemiluminescence (Millipore, Burlington, MA, USA). Images were obtained using ChemiDoc XRS (Bio-Rad). The relative band intensity was quantified using the Image Lab Analyzer software (Bio-Rad, Hercules, CA). The antibodies used in the present research were as follows: rabbit anti-β-Tubulin antibody and rabbit anti-GAPDH antibody (1:2000, Servicebio, Wuhan, China); rabbit anti-α-SMA antibody (1:1000, SAB, Maryland, USA); rabbit anti-sEH antibody (1:5000, Abcam, USA); rabbit anti-COX-2 antibody (1:1000, Servicebio); rabbit anti-Collagen Type I antibody (1:1000, Proteintech, Rosemont, USA); rabbit anti-Collagen Type III antibody (1:1000, Proteintech); rabbit anti-SFTPC antibody (1:1000, Abcam); rabbit anti-p53 antibody (1:3000, Proteintech).
Zhang C.Y., Duan J.X., Yang H.H., Sun C.C., Zhong W.J., Tao J.H., Guan X.X., Jiang H.L., Hammock B.D., Hwang S.H., Zhou Y, & Guan C.X. (2019). COX-2/sEH dual inhibitor PTUPB alleviates bleomycin-induced pulmonary fibrosis in mice via inhibiting senescence. The FEBS journal, 287(8), 1666-1680.
+ Open protocol
+ Expand
3

Immunohistochemical Analysis of PPARα and P53

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemical analysis was performed using the Histone Simple Stain Kit (Nichirei, Tokyo, Japan), according to the manufacturer's instructions. Paraffin-embedded sections were deparaffinised with xylene and then rehydrated in a descending series of ethanol washes. The sections were treated for 15 minutes with 3% H2O2 in methanol to inactivate endogenous peroxidases and then incubated at room temperature for 1 hour with primary antibodies to PPARα (rabbit anti-PPARα antibody, 1:200; Proteintech, Wuhan, China) and P53 (rabbit anti-P53 antibody, 1:200; Proteintech). Tissue sections were observed with a microscope (Olympus, Tokyo, Japan).
Pei Z., Deng S., Xie D., Lv M., Guo W., Liu D., Zheng Z, & Long X. (2018). Protective role of fenofibrate in sepsis-induced acute kidney injury in BALB/c mice. RSC Advances, 8(50), 28510-28517.
+ Open protocol
+ Expand
4

Western Blot Analysis of Kidney Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were extracted from kidney tissue using radio immunoprecipitation assay buffer (P0013B; Beyotime, Shanghai, China). Samples were electrophoresed on a 10% SDS-PAGE gel, and proteins were transferred to polyvinylidene fluoride membrane (Immobilon, Millipore, Billerica, MA, USA). Membranes were blocked in Tris-buffered saline with 0.1% Tween-20 (TBS-T) containing 5% skim milk and then incubated in primary antibody diluent (P0023A; Beyotime) and gently shaken overnight at 4 °C. Primary antibodies against PPARα (rabbit anti-PPARα antibody, 1:1000; Proteintech), P53 (rabbit anti-P53 antibody, 1:1000; Proteintech), and anti-β-actin (1:1000; Cell Signaling Technology) were utilised. Membranes were then incubated with secondary antibody (anti-rabbit Ig-G, 1:1000; Cell Signaling Technology for 1 hour). This analysis was carried out independently three times. Protein levels are expressed as protein/β-actin ratios to minimise loading differences. The relative signal intensity was quantified using NIH ImageJ software.
Pei Z., Deng S., Xie D., Lv M., Guo W., Liu D., Zheng Z, & Long X. (2018). Protective role of fenofibrate in sepsis-induced acute kidney injury in BALB/c mice. RSC Advances, 8(50), 28510-28517.
+ Open protocol
+ Expand
5

Western Blot Analysis of Cardiac Fibrosis Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cardiac tissues were harvested, and protein extracts prepared, according to established methods. Extracts were separated in sodium dodecyl sulphate–polyacrylamide electrophoresis gels (8–15%) and transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, Bedford, MA, USA). The membranes were blocked with 5% milk and then incubated with indicated primary antibodies at 4 °C overnight. Primary antibodies against TGF-β (rabbit anti-TGF-β antibody, 1 : 1000; Proteintech, Wuhan, China), Smad3 (rabbit anti-Smad antibody, 1 : 1000; Proteintech), collagen I (rabbit anti-collagen I antibody, 1 : 1000; Proteintech), collagen III (rabbit anti-collagen III antibody, 1 : 1000; Proteintech), α-SMA (rabbit anti-α-SMA antibody, 1 : 1000; Proteintech), P53 (rabbit anti-P53 antibody, 1 : 1000; Proteintech), bcl-2(rabbit anti-bcl-2 antibody, 1 : 1000; Proteintech), anti-β-actin (1 : 1000; Cell Signaling Technology). After washing, the membranes were incubated with the appropriate secondary antibody (anti-rabbit Ig-G, 1 : 1000; Cell Signaling Technology) for 1 hour. This analysis was carried out independently three times. Protein levels were expressed as protein/β-actin ratios to minimise loading differences. The relative signal intensity was quantified using NIH ImageJ software.
Pei Z., Hu J., Bai Q., Liu B., Cheng D., Liu H., Na R, & Yu Q. (2018). Thymoquinone protects against cardiac damage from doxorubicin-induced heart failure in Sprague-Dawley rats. RSC Advances, 8(26), 14633-14639.
+ Open protocol
+ Expand
6

Immunohistochemical Analysis of PPAR-α and P53

Check if the same lab product or an alternative is used in the 5 most similar protocols
We followed the methods of Pei et al. 2018 (14) , immunohistochemistry was carried out using Histone Simple Stain Kit (Nichirei, Tokyo, Japan). In brief, paraffin-embedded sections were deparaffinised using xylene followed by a descending series of ethanol washes to rehydrate. Inactivation of endogenous peroxidases was conducted by treating sections with 3% H 2 O 2 in methanol for 15 minutes. Then the sections were incubated with primary antibodies to PPAR-α (rabbit anti-PPAR-α antibody, 1:200; Proteintech, Wuhan, China) and P53 (rabbit anti-P53 antibody, 1:200; Proteintech) for 1 hour at room temperature. Observation of myocardium sections was carried out using a microscope (Olympus, Tokyo, Japan).
Lv M., Xie D, & Long X. (2022). The effect of fenofibrate, a peroxisome proliferator-activated receptor α agonist, on cardiac damage from sepsis in BALB/c mice. Cellular and molecular biology (Noisy-le-Grand, France), 67(6).
+ Open protocol
+ Expand
7

Cardiac Tissue Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolation of whole-cell lysates from cardiac tissues was performed using radio immunoprecipitation assay buffer (P0013B; Beyotime, Shanghai, China) as described before (14) . Proteins were separated by electrophoresis on 10% SDS-PAGE gel and transferred to polyvinylidene fluoride membrane (Immobilon, Millipore, Billerica, MA, USA). Then the membranes were blocked in Tris-buffered saline with 0.1% Tween-20 (TBS-T) containing 5% skim milk, and exposed to diluent (P0023A; Beyotime) of primary antibodies against PPARα (rabbit anti-PPAR-α antibody, 1:1000; Proteintech), P53 (rabbit anti-P53 antibody, 1:1000; Proteintech), anti-GAPDH (1:1000; Cell Signaling Technology), and gently shaken overnight at 4°C. Membranes were then exposed to secondary antibody (anti-rabbit Ig-G, 1:1000; Cell Signaling Technology) for one hour. This analysis was carried out independently three times. The intensities were analyzed using NIH Image J software and levels of protein were normalized to GAPDH.
Lv M., Xie D, & Long X. (2022). The effect of fenofibrate, a peroxisome proliferator-activated receptor α agonist, on cardiac damage from sepsis in BALB/c mice. Cellular and molecular biology (Noisy-le-Grand, France), 67(6).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!