The carbapenem-sensitive isolate 1104–65 and carbapenem-resistant isolate 1104–75 used in this study were collected from the stool of the same outpatient in Fifth Affiliated Hospital, Southern Medical University in Conghua District, Guangzhou. Strain 1104–65 was isolated from the patient’s stool in November 2021, and 1104–75 was isolated from another stool specimen 10 days later. Extract a sufficient amount of stool sample and use an inoculation loop to inoculate it onto blood agar plates, SS medium, and MacConkey agar plates. The typical colony morphology of Salmonella on SS medium is colorless, transparent, and black in the center. After incubation at 37°C for 16–18 hours, a single colony was selected and drawn on a blood agar plate to obtain pure isolates for identification and antimicrobial susceptibility tests. Isolates were analyzed and identified by the VITEK-2 COMPACT automatic microbial identification system (bioMérieux, Marcy-l'Étoile, France). Salmonella serotyping was conducted by using the slide agglutination test with specific antisera (Tianrun, Ningbo, China) according to the manufacturer’s instructions.
Vitek 2 compact automatic microbial identification system
Manufactured by bioMérieux
Sourced in France
The VITEK-2 COMPACT is an automated microbial identification system designed for rapid and accurate identification of microorganisms. It utilizes advanced technology to automate the process of identifying bacteria and yeast from clinical samples.
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Lab products found in correlation
4 protocols using vitek 2 compact automatic microbial identification system
1
Isolation and Identification of Salmonella Isolates
2
Identification and Antibiotic Profiling of ESBL-Producing E. coli
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A total of 374 non-repetitive ESBLs-producing E. coli strains isolated from patients in two hospitals in Guangzhou were identified by the advanced expert system (AES) of the VITEK-2 COMPACT Automatic Microbial Identification System (bioMérieux, Marcy-l’Étoile, France). Minimal inhibitory concentrations (MICs) including the ESBLs-resistant phenotype were determined using the VITEK-2 Automated Susceptibility System (Spanu et al., 2006 (link)). The MICs of the isolates against amoxicillin/clavulanic acid (AMC), piperacillin/tazobactam (TZP), amikacin (AMK), compound sulfamethoxazole (SXT), ceftriaxone (CRO), ceftazidime (CAZ), cefepime (FEP), ertapenem (ETP), imipenem (IPM), and levofloxacin (LVX) were determined by agar dilution method, and the results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI/NCCLS M100-S30) [Clinical and Laboratory Standards Institute [CLSI], 2021 ]. All isolates were collected in two tertiary hospitals from 2012 to 2017 and 2020, while the isolates from 2018 to 2019 were not collected, so they were not included in this study. E. coli ATCC 25922 was used as a quality control strain. E. coli C600 was used as the recipient strain in the conjugation experiments.
3
Identification of Candida Species in Vaginal Secretions
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Vaginal secretions were cultured on Sabouraud dextrose agar (Oxoid, UK) at both 25°C and 37°C for 2 or 3 days, respectively. Putative positive colonies were white or cream in color with glossy and smooth surfaces. If no colonies were evident within 2 weeks, the sample was considered negative. Yeast-like colonies were Gram-stained to exclude any bacterial colonies that had been mistakenly identified as yeast. Colonies were then inoculated on CHROMagar medium (France) at 35°C for 24 - 48 hours. Green or emerald green colonies were identified as C. albicans, blue-gray colonies were identified as C. tropicalis, and purple-embossed colonies with smooth and glossy surfaces were identified as C. glabrata. Pink-to-purple large flat colonies with rough edges were identified as C. krusei, while colonies of the other phenotypes were identified as other Candida spp. These atypical isolates were further identified using the VITEK 2 compact automatic microbial identification system (bioMerieux, France).
4
Isolation and Characterization of Strain SR33
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Strain SR33 was isolated from a fecal sample of an old patient. This patient was hospitalized due to occasional fever and diarrhea. During hospitalization, cefixime was ineffective against this infection, but it improved after treatment with levofloxacin. SR33 was identified by the VITEK-2 COMPACT automatic microbial identification system (bioMérieux, Marcy-l’Étoile, France), and its serotype was confirmed by slide agglutination technique (Kauffmann-White-Le Minor scheme) (Grimont and Weill, 2007 )
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