Plc γ1 pab

The following antibodies were used in this study: p-PLC-γ1(Ser1248) rabbit mAb (cat# 8713), PLC-γ1 pAb (cat# 2822S), β-actin rabbit mAb (cat# 4970), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (cat# 7076), as well as HRP-labeled goat anti-rabbit IgG (cat# 7074) were purchased from Cell Signaling Technology (Danvers, MA, USA). BoHV-1 gD mAb (cat# 1B8-F11) and goat anti-BoHV-1 serum (cat# PAB-IBR) were provided by VMRD Inc. (Pullman, WA, USA). EGFR pAb (cat# A11577) and GOLGA1 rabbit pAb (cat# A14688) were bought from Abclonal Technology (Woburn, MA, USA). GP73/GOLPH2 mouse mAb (cat# 66331-1-lg) was provided by Proteintech (Rosemont, IL, USA). Donkey anti-goat IgG H&L (HRP) (ca# ab97110), Alexa Fluor 647-conjugated goat pAb to rabbit IgG (cat# ab150079), and Alexa Fluor 488-conjugated donkey anti-goat IgG (cat# ab150129) were provided by Abcam (Cambridge, UK). Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) (cat# A-11008) and Alexa Fluor 633-conjugated goat anti-mouse IgG (H + L) (Invitrogen, cat# A-21052) were provided by Invitrogen Life Technologies (Waltham, MA, USA).

The following antibodies were used in this study: p-PLC-γ1(Ser1248) rabbit mAb (cat# 8713), PLC-γ1 pAb (cat# 2822S), β-actin rabbit mAb (cat# 4970), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (cat# 7076), as well as HRP-labeled goat anti-rabbit IgG (cat# 7074) were purchased from Cell Signaling Technology (Danvers, MA, USA). BoHV-1 gD mAb (cat# 1B8-F11) and goat anti-BoHV-1 serum (cat# PAB-IBR) were provided by VMRD Inc. (Pullman, WA, USA). EGFR pAb (cat# A11577) and GOLGA1 rabbit pAb (cat# A14688) were bought from Abclonal Technology (Woburn, MA, USA). GP73/GOLPH2 mouse mAb (cat# 66331-1-lg) was provided by Proteintech (Rosemont, IL, USA). Donkey anti-goat IgG H&L (HRP) (ca# ab97110), Alexa Fluor 647-conjugated goat pAb to rabbit IgG (cat# ab150079), and Alexa Fluor 488-conjugated donkey anti-goat IgG (cat# ab150129) were provided by Abcam (Cambridge, UK). Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) (cat# A-11008) and Alexa Fluor 633-conjugated goat anti-mouse IgG (H + L) (Invitrogen, cat# A-21052) were provided by Invitrogen Life Technologies (Waltham, MA, USA).

Phospho-EGFR-Y1068 monoclonal Ab (mAb) (cat# 2234S), Akt mAb (cat# 9272S), phospho-Akt (Ser473) mAb (cat# 9271S), phospho-PLC-γ1(Ser1248) mAb (cat# 8713), PLC-γ1 pAb (cat# 2822S), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mAb (cat# 2118), HRP labeled goat anti-rabbit IgG and goat anti-mouse IgG were purchased from Cell Signaling Technology (Danvers, MA, USA). EGFR polyclonal antibody (pAb) (cat# A11577) and β-Tubulin Rabbit pAb (cat# AC015) were provided by ABclonal Technology (Woburn, MA, USA). All the primary antibodies employed for Western blots were at a 1:1000 dilution. The chemical inhibitors used in this study, U73122 (cat# HY-13419), Gefitinib [28 (link)] (cat# HY–50895), and LY294002 [28 (link)] (cat# HY–10108), were provided by MedChemExpress (Monmouth Junction, NJ, USA). U73122 is an inhibitor that can specifically inhibit PLC-γ1 phosphorylation at Ser1248 [19 (link),29 (link)]. Gefitinib is an EGFR inhibitor that can selectively inhibit EGFR tyrosine kinase activity by binding to the adenosine triphosphate (ATP)-binding site [30 (link)]. LY294002 can specifically inhibit Akt phosphorylation by inactivation of its direct activator phosphoinositide 3-kinases (PI3K) [31 ].