Ck mb

Liver damage was diagnosed if the biochemical analyzer (Abbott Laboratories, Abbott Park, IL, USA) showed abnormal values of total bilirubin (TBil), indirect bilirubin (IBil), serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and/or alkaline phosphatase (ALP); similarly, myocardial damage was diagnosed if there were elevated levels of serum creatine kinase (CK), creatine kinase (CK) isoenzyme MB (CK-MB), and/or lactate dehydrogenase (LDH). Levels of C-reactive protein (CRP) were also detected by the biochemical analyzer (Abbott). Blood cell count analysis was performed using a Mindray BC-6900 hematology analyzer (Mindray Medical International Ltd., Shenzhen, China). The reference values of these markers were set by children’s hospital. Differences of various indicators in children of different ages were analyzed.

Blood samples from patients were collected from radial or femoral site of arterial catheter and processed within 1 h. Blood samples were always obtained around midday to limit circadian oscillations of neutrophil phenotypes. Haematological parameters were determined in EDTA-anticoagulated blood on a Cell-Dyn 3700 (Abbott Laboratories). Platelet poor plasma was obtained after two consecutive centrifugations at 1750 g during 15 min at room temperature. Sera were obtained following one centrifugation at 1750 g during 15 min at RT. Plasma and sera were stored at −80 °C until analysis. IL-6 and S100A9 were measured on EDTA-plasma using a Luminex assay kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. Nucleosome levels were quantified on EDTA-plasma with the Cell Death Detection ELISA plus kit (Sigma-Aldrich, Overijse, Belgium). Myeloperoxidase quantity and activity were analysed on EDTA-plasma by a method adapted from Franck et al. [11 (link)] using a human myeloperoxidase (MPO) Quantikine ELISA kit (R&D Systems). hs-CRP, CK-MB (Abbott Laboratories, Wavre, Belgium) and high-sensitive troponin T (hs-TnT) (Roche, Machelen, Belgium) were measured in sera by routine immunoassays.