Immunoblots were performed as previously described (16 (link)). Briefly, total protein extracts were prepared by homogenizing tissues in lysis buffer with protease inhibitor (Sigma-Aldrich, MO). Proteins in lysis buffer were mixed with SDS sample buffer, resolved in an SDS-polyacrylamide gel, and transferred to polyvinylidene fluoride membrane. After immunoblocking, membranes were incubated overnight with TRPV1 rat polyclonal antibody (cat# ACC-030; Alomone Labs, Jerusalem, Israel) or actin rabbit polyclonal antibody (cat#A2103; Sigma-Aldrich) at 4°C. After incubating with appropriate horseradish peroxidase–conjugated secondary antibodies for 1 hour, chemiluminescence with SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific, Waltham, MA) was used to detect protein expression (47 (link)).
Actin rabbit polyclonal antibody
Manufactured by Merck Group
Sourced in Israel
The Actin rabbit polyclonal antibody is a laboratory reagent used for the detection and study of actin, a crucial cytoskeletal protein found in eukaryotic cells. This antibody is produced in rabbits and recognizes both filamentous and monomeric forms of actin.
Automatically generated - may contain errors
Lab products found in correlation
Supersignal west dura extended duration substrate, by Thermo Fisher Scientific (1 mentions)
Protease inhibitor, by Merck Group (1 mentions)
Fibronectin, by Thermo Fisher Scientific (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
Anti gst monoclonal antibody, by BioLegend (1 mentions)
Gfp polyclonal antibody, by Thermo Fisher Scientific (1 mentions)
Alpha tubulin clone b 5 1 2, by Merck Group (1 mentions)
3 protocols using actin rabbit polyclonal antibody
1
Immunoblot Analysis of TRPV1 Protein Expression
2
Antibody Validation for Cell Signaling Proteins
3
Antibody Characterization for Cell Biology
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SPARC monoclonal antibody (clone AON-5031; 1:200 Immunofluorescence microscopy (IF), 1:1000 Western blot (WB)) was purchased from Cambridge Bioscience (Cambridge, UK). Alpha-tubulin (clone B-5-1-2; 1:3000 WB), Collagen I (clone COL-I; 1:200 IF), and Collagen IV (clone COL-94; 1:200 IF) monoclonal antibodies and actin rabbit polyclonal antibody (1:3000 WB) were purchased from Sigma-Aldrich. Purified ß1 integrin polyclonal antibody (clone Poly6004; 1:1000 WB) and anti-GST monoclonal antibody (1:3000 WB) were purchased from Biolegend (Uithoorn, Netherlands). Fibronectin (1:300 IF) and FAK monoclonal antibodies (1:1000 WB) and GFP polyclonal antibody (1:2000 WB) were purchased from Invitrogen (Paisley, UK). Affinity purified polyclonal antibody directed against TGFBI was produced by immunizing rabbits with a C-terminal peptide of human TGFBI (aa 498–683) (1:1000 WB). All antibody production was performed in collaboration with Cambridge Research Biochemicals (Cleveland, UK). TGFBI polyclonal antiserum (1:150 IF) was a kind gift from Dr. Ching Yuan (University of Minnesota, Minnesota, USA). Paclitaxel was purchased from Sigma-Aldrich, cat. no. T7402 (Dorset, UK).
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