β nicotinamide adenine dinucleotide sodium salt nad

Manufactured by Merck Group

Sourced in United States

β-nicotinamide adenine dinucleotide sodium salt (NAD+) is a coenzyme found in all living cells. It is involved in redox reactions, serving as an electron acceptor in various metabolic processes.

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3 protocols using β nicotinamide adenine dinucleotide sodium salt nad

Polymicrobial Biofilm Formation on Root Canals

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The following microorganisms were separately precultured and suspended for 18 h:

Enterococcus faecalis ATCC 29212

Candida albicans ATCC 76615

Streptococcus gordonii ATCC 10558 and Actinomyces oris ATCC 43146

Thirty autoclaved roots were randomly allocated to each group of microorganisms and inoculated for three days at 37 °C. Groups 1 and 2 were cultured under aerobic conditions. Group 3 was cultured with 10% CO₂. Tryptic soy agar (TSA) plates with 5% sheep blood were used for the cultivation of the microorganisms. Then, suspensions of E. faecalis ATCC 29212 and C. albicans ATCC 76615 were prepared in a 0.9% sodium chloride solution (McFarland 4), which was then diluted, at a ratio of 1:9, with a brain-heart infusion broth (Oxoid Limited, Basingstoke, UK). Wilkins-Chalgren anaerobe broth (Oxoid Limited) supplemented with 5 mg/l β-nicotinamide adenine dinucleotide sodium salt (NAD) (Sigma-Aldrich, St. Louis, MO, USA) was used for the suspension of S. gordonii ATCC 10558 and A. oris ATCC 43146 (mixed at a ratio of 1:2). The nutrient broths were renewed daily. At the beginning of the microbiological experiments, sterility was monitored by sampling and culturing on TSA plates under both aerobic and anaerobic conditions as described above. Cultivation was performed over 4 days.

Eggmann F., Vokac Y., Eick S, & Neuhaus K.W. (2020). Sonic irrigant activation for root canal disinfection: power modes matter!. BMC Oral Health, 20, 102.

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Alcohol Dehydrogenase Enzyme Assay

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Alcohol dehydrogenase (ADH) from Saccharomyces cerevisiae, β-nicotinamide adenine dinucleotide sodium salt (NAD⁺), phosphate buffer solution, β-alanine, triethanolamine, 2-mercaptoethanol, deuterated chloroform (CDCl₃) containing trimethylsilane (TMS), octadecylphosphonic acid (ODPA), trioctylphosphine oxide (TOPO), trioctylphoshine (TOP), octanoic acid, crotonaldehyde (CA), and butyraldehyde (BA) were purchased from Sigma-Aldrich. Acetaldehyde (AA), n-octane, and palladium acetylacetonate were purchased from Acros Organics. (1,5-Cyclooctadiene)-dimethylplatinum(II), cadmium oxide, glycerol tributyrate (GT), and sulfur were purchased from Alfa Aesar. β-Nicotinamide adenine dinucleotide disodium salt hydrate (NADH) and 2-ethylhexenal (2-EH) were purchased from TCI Chemicals. Butanol (BuOH), 2-propanol, acetone, dextrose, and toluene were purchased from Fisher Scientific. Yeast nitrogen base (w/o amino acids and ammonium sulfate) was purchased from Beckton, Dickinson, and Company. Active dry yeast was purchased from ACH Food Companies, Inc. Ethanol (EtOH) was purchased from Decon Laboratories, Inc. Deuterium oxide (D₂O) and sodium 2,2-dimethyl-2-silapentane-5-sulfonate (DSS) were purchased from Cambridge Isotope Laboratories, Inc.

Hafenstine G.R., Harris A.W., Ma K., Cha J.N, & Goodwin A.P. (2017). Conversion of Ethanol to 2-Ethylhexenal at Ambient Conditions Using Tandem, Biphasic Catalysis. ACS sustainable chemistry & engineering, 5(11), 10483-10489.

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Chitosan-based Cytotoxicity Assays

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The following materials were purchased from Sigma-Aldrich (St. Louis, MO): chitosan (85% deacetylated), pyrocatechol, sodium L-lactate, β-nicotinamide adenine dinucleotide sodium salt (NAD⁺), pyocyanin (PYO) and hexaammineruthenium chloride (Ru³⁺). 1,1’Ferrocenedimethonal (Fc) was purchased from Acros Organics (New Jersey, NJ). All mammalian cell culture media, LIVE-DEAD viability/cytotoxicity kit and Pierce LDH cytotoxicity assay kits were obtained from Thermo Fisher Scientific (Waltham, MA).

Shang W., Liu Y., Kim E., Tsao C.Y., Payne G.F, & Bentley W.E. (2018). Selective assembly and functionalization of miniaturized redox capacitor inside microdevices for microbial toxin and mammalian cell cytotoxicity analyses. Lab on a chip, 18(23), 3578-3587.

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