Specific antibodies used, were targeting: P2X7 (APR-008, Alomone); P2X4 (APR-002, Alomone); PDHX (H-130, Santa Cruz); p-p38 Thr180/Tyr182 (28B10, Cell Signalling Technologies); E-selectin (NBP1-45545, Novus Biologicals); CD31-AlexaFluor488 (Clone Mec13.3, Biolegend), and CD39-FITC (Clone A1, Biolegend). HRP-conjugated secondary antibodies were from Dako. AlexaFluor conjugated antibodies, TO-PRO-3 and aqueous mounting media (Prolong Gold Antifade Mountant) were from Invitrogen. All other reagents were from Sigma Aldrich unless specified.
E selectin
Manufactured by Novus Biologicals
E-selectin is a cell adhesion molecule that mediates the rolling and tethering of leukocytes to the vascular endothelium. It plays a crucial role in the initial steps of the inflammatory response.
Automatically generated - may contain errors
Lab products found in correlation
P p38 thr180 tyr182, by Cell Signaling Technology (1 mentions)
Apr 008, by Alomone (1 mentions)
Apr 002, by Alomone (1 mentions)
Cd31 alexafluor488, by BioLegend (1 mentions)
Cd39 fitc, by BioLegend (1 mentions)
To pro 3, by Thermo Fisher Scientific (1 mentions)
Prolong gold antifade mountant, by Thermo Fisher Scientific (1 mentions)
Hrp conjugated secondary antibody, by Agilent Technologies (1 mentions)
Alexa fluor conjugated antibody, by Thermo Fisher Scientific (1 mentions)
Gamma h2ax, by Novus Biologicals (1 mentions)
Donkey anti goat igg h l alexa fluor 568, by Thermo Fisher Scientific (1 mentions)
Phospho erk5, by Cell Signaling Technology (1 mentions)
Icam 1, by Novus Biologicals (1 mentions)
Alexa fluor 488 goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions)
Anti rabbit igg hrp, by Cell Signaling Technology (1 mentions)
Anti mouse igg hrp, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
2 protocols using e selectin
1
Multiparametric Immunohistochemical Analysis
2
Antibody Sources for Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies to KLF2 (Cat # ab203591), KLF4 (Cat # ab106629), and MEF2 (Cat # ab64644) were purchased from Abcam (Burlingame, CA, USA). Antibodies to TM (Cat # NBP1-95319), ICAM-1 (Cat # NBP2-22541), eNOS (Cat # NB300–500), E-selectin (Cat #NBP1-45545), and gamma H2AX (Cat # NB100-384) were procured from Novus Biologicals. Antibodies to ERK5 (Cat #3372), phosphoERK5 (Cat #3371), and β-actin (Cat #4970) were obtained from Cell Signaling Technology Inc. (Beverly, MA, USA).
Anti-rabbit IgG-HRP (Cat #7074) and anti-mouse IgG-HRP (Cat #7076) secondary antibodies were purchased from Cell Signaling Technology Inc. Fluorochrome-conjugated goat anti-rabbit IgG (H + L)-Alexa Fluor 488 (Cat # A11034), donkey anti-goat IgG (H + L)-Alexa Fluor 568 (Cat # A11057), and goat anti-mouse IgG (H + L)-Alexa Fluor 488 (Cat # A11029) were procured from Invitrogen (ThermoFisher Scientific).
Anti-rabbit IgG-HRP (Cat #7074) and anti-mouse IgG-HRP (Cat #7076) secondary antibodies were purchased from Cell Signaling Technology Inc. Fluorochrome-conjugated goat anti-rabbit IgG (H + L)-Alexa Fluor 488 (Cat # A11034), donkey anti-goat IgG (H + L)-Alexa Fluor 568 (Cat # A11057), and goat anti-mouse IgG (H + L)-Alexa Fluor 488 (Cat # A11029) were procured from Invitrogen (ThermoFisher Scientific).
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