Jnm ecs400 400 mhz

Solvents and reagents were purchased from Nacalai Tesque, Inc. (Kyoto, Japan), Tokyo Chemical Industry, Co., Ltd. (Tokyo, Japan), Fujifilm Wako Pure Chemical Corporation (Osaka, Japan), Kanto Chemical, Co., Inc. (Tokyo, Japan), Merck (Darmstadt, Germany), and Chemescene (Monmouth Junction, NJ, USA), and used as provided. CO-1686 was purchased from AdooQ BioScience (Irvine, Canada). Gefitinib was purchased from Fujifilm Wako Pure Chemical Corporation (Osaka, Japan). Thin-layer chromatography (TLC) was performed on silica plates 60 F₂₅₄ (Merck, Darmstadt, Germany).
Nuclear magnetic resonance (NMR) spectra were recorded on a JNM-ECS400 (400 MHz) or JNM-ECA600 (600 MHz) spectrometer (JEOL Ltd., Tokyo, Japan). The mass spectrometry was performed on a JMS-T700 (JEOL Ltd., Tokyo, Japan) on fast atom bombardment mass spectrometry (FAB-MS). HPLC analyses and purification were carried out on a Shimadzu Prominence HPLC system (Shimadzu Corp., Kyoto, Japan). The optical density in WST-8 assays were measured on an Infinite^® F200 Pro microplate reader (TECAN, Männedorf, Switzerland). Radioactivity was determined using an auto gamma counter ARC-7010B (Hitachi, Ltd., Tokyo, Japan).

DOX was purchased from LC Laboratories (Woburn, MA, USA). 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (PEG-DSPE) were purchased from NOF Corporation (Tokyo, Japan). Cholesterol (Chol) was purchased from Sigma Aldrich (St. Louis, MO, USA). [¹²⁵I]Sodium iodide (644 GBq/mg) was purchased from PerkinElmer (Waltham, MA, USA). Other reagents were of reagent grade and used as received. Thin layer chromatography (TLC) was performed on silica plates 60 F254 (Merck, Darmstadt, Germany). Nuclear magnetic resonance (NMR) spectra were recorded on a JNM ECS400 (400 MHz) or JNM ECA600 (600 MHz) spectrometer (JEOL Ltd., Tokyo, Japan). The mass spectrometry was performed on a JMS T700 (JEOL Ltd.,) on electrospray ionization mass spectrometry (ESI-MS). HPLC analyses and purification were carried out on a Shimadzu Prominence HPLC system (Shimadzu Corp., Kyoto, Japan). Radioactivity was determined using an auto gamma counter ARC 7010B (Hitachi, Ltd., Tokyo, Japan). A colorectal adenocarcinoma cell line, Colon 26, was obtained from Cell Resource Centre for Biomedical Research in Tohoku University.