Indicated HCC cells (5 × 104 cells) were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in PBS. Expression of E-cadherin and vimentin was detected using each respective primary antibody and visualized with Alexa Fluor 488-conjugated secondary antibodies (Invitrogen, USA). Nuclei were counterstained with Hoechst 33258. All scale bars represent 200 μm. All images were observed by DeltaVision RT wide-field epifluorescence microscope imaging system and softWoRxs image analysis program (Applied Precision, USA).
Deltavision rt wide field epifluorescence microscope imaging system
Manufactured by Cytiva
Sourced in United States
The DeltaVision RT wide-field epifluorescence microscope imaging system is a high-performance microscope designed for fluorescence imaging applications. It features a wide-field illumination system and a high-resolution imaging sensor, enabling the capture of detailed images of fluorescently labeled samples.
Automatically generated - may contain errors
4 protocols using deltavision rt wide field epifluorescence microscope imaging system
1
Immunofluorescence Analysis of Cell-Cell Junctions
2
Evaluating TGF-β1 and KY-05009 Effects
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A549 cells (1×105 cells/mL) were incubated on a 1% gelatin-coated slide glass for 24 h. After serum starvation for 24 h, cells were treated with TGF-β1 and KY-05009 for 48 h. All cells were fixed with 2% formaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in PBS. Expression of p-Smad2, E-cadherin, and vimentin was detected using each respective primary antibody, and visualized with Alexa Fluor 488-conjugated secondary antibodies (Invitrogen). Nuclei were counterstained with Hoechst 33258. All images were observed by DeltaVision RT wide-field epifluorescence microscope imaging system and softWoRxs image analysis program (Applied Precision, NW, USA).
3
Immunofluorescence Analysis of EMT Markers
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Indicated HCC cells (5 × 104 cells) were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in PBS. Expression of E-cadherin, vimentin, ZO-1, N-cadherin, Twist1, SIK1, and β-catenin was detected using each respective primary antibody and visualized with Alexa Fluor 488-conjugated secondary antibodies (Invitrogen, USA). Nuclei were counterstained with Hoechst 33258. All scale bars represent 200 μm. All images were captured by DeltaVision RT wide-field epifluorescence microscope imaging system and analyzed by softWoRxs image analysis program (Applied Precision, USA).
4
TGF-β1 and Gefitinib Modulate Vimentin Expression
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A549 cells were incubated on a 1% gelatin-coated slide glass for 24 h. Serum-deprived A549 cells were treated with TGF-β1 and gefitinib for 72 h. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in PBS. Expression of vimentin was detected using a primary antibody and visualized with Alexa Fluor 488-conjugated secondary antibodies. Nuclei were counterstained with DAPI. All images were observed by a DeltaVision RT wide-field epifluorescence microscope imaging system and softWoRxs image analysis program (Applied Precision, United States). The relative fluorescence intensity of vimentin was quantified using ImageJ software.
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