Fluorescence conjugated alexa fluor secondary antibodies

Bromodichloromethane (BDCM), a known substrate for CYP2E1-mediated oxidative stress in the liver and corn oil were purchased from Sigma-Aldrich (St. Louis, MO). Anti–α-SMA, anti–IL-1β, anti–TNF-α, anti-TLR-4, Anti-3-Nitrotyrosine (3-NT), anti-P47phox and anti-GP91phox primary antibodies were purchased from Abcam (Cambridge, MA). Species-specific biotinylated conjugated secondary antibody and streptavidin -HRP were purchased from Vectastain Elite ABC kit (Vector Laboratories, Burlingame, CA). Wild-type and gene-specific KO mice were purchased from The Jackson Laboratories (Bar Harbor, ME). NOX2-siRNA, RNAiMAX-Lipofectamine, Fluorescence-conjugated (Alexa fluor) secondary antibodies and ProLong Gold antifade mounting media with DAPI were bought from Thermo Fisher Scientific (Waltham, MA). Animal diets were purchased from Research Diets (New Brunswick, NJ). All other chemicals were purchased from Sigma Aldrich unless otherwise specified. Paraffinized tissue sections on slides were done by IRF, University of South Carolina School of Medicine and AML laboratories (Baltimore, MD).

We purchased PB and permethrin from Sigma-Aldrich (St. Louis, MO). Anti-RAGE, anti-Claudin 5, anti-HMGB1, anti-IL-1β, and anti-ASC-2 were purchased from Santacruz Biotechnology (Dallas, TX), Anti-BDNF from cell signaling technology (Danvers, MA), while anti-NLRP3, anti-3-nitrotyrosine, anti-IL-6, anti-IL-18, anti-TMEM 119 primary antibodies were purchased from Abcam (Cambridge, MA). Species specific biotinylated conjugated secondary antibodies and Streptavidin-HRP (Vectastain Elite ABC kit) were purchased from Vector Laboratories (Burlingame, CA). Fluorescence conjugated (Alexa Fluor) secondary antibodies, ProLong Diamond antifade mounting media with DAPI and Pierce LAL chromogenic endotoxin quantitation kit were bought from Thermo Fisher Scientific (Waltham, MA) while enzyme-linked immunosorbent assay (ELISA) kits were purchased from ProteinTech (Rosemond, IL). Unless otherwise specified, all other chemicals used were purchased from Sigma. Paraffin-embedding of tissue and sectioning were done by AML laboratories (Baltimore, MD) and at the Instrument Resources Facility, University of South Carolina School of medicine (Columbia, SC). Microbiome analysis was done by Cosmos ID (Rockville, MD).