Mannitol salt phenol red agar
Mannitol Salt Phenol Red Agar is a selective and differential culture medium used for the isolation and identification of Staphylococcus species from clinical and non-clinical samples. It contains mannitol as a carbohydrate source and phenol red as a pH indicator. The medium allows for the growth of Staphylococcus species while inhibiting the growth of most other bacteria.
Lab products found in correlation
8 protocols using mannitol salt phenol red agar
Antimicrobial Poly(lactic acid) Granules
Coculture of S. aureus and E. coli
Wound-like Media Mono/Co-culture Assay
Wound-like Media Mono/Co-culture Assay
Isolation and Identification of Foodborne Staphylococci
Enumeration of Microbial Populations in Food
Screening of Microbial Enzymatic Activities
Isolation and Identification of S. aureus from Street Food
Isolation of S. aureus from street food samples was carried out as the method reported previously.22 First, 10 g of each sample was transferred separately to a sterile Stomacher bag and 90 mL of NaCl (Merck, Germany) 0.9% solution were added. The sample underwent homogenization in a stomacher (Seward, England) operating at 200 rpm for 5 min. Then, a 10-fold dilution of the sample was prepared using sterile NaCl (Merck, Germany) 0.9%, and 0.1 mL of various dilutions was spread onto the surface of mannitol salt phenol red agar (Merck, Germany) plates. The plates were then incubated at 35 ± 2 °C for 24 h. Yellow colonies with yellow zones were transferred to Baird-Parker agar (Merck, Germany) medium with tellurite egg yolk (Merck, Germany). Subsequently, the plates were incubated at 35 ± 2 °C for 24 h. Black, shiny, convex colonies surrounded by a lightening halo of the egg yolk were used for biochemical tests, including Gram stain, catalase test, indole test, Methyl red-Voges Proskauer test, oxidase test, test for fermentation of glucose, lactose, and sucrose, and coagulase test.
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