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Merestinib

Manufactured by Eli Lilly
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Sourced in United States

Merestinib is a laboratory equipment product manufactured by Eli Lilly. It is designed for use in various research and analytical applications.

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Lab products found in correlation

Foxm1, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Cyclin a2, by Cell Signaling Technology (1 mentions) P70s6k, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Abemaciclib, by Eli Lilly (1 mentions)

2 protocols using merestinib

1

Characterizing Abemaciclib and Merestinib Mechanisms

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A CDK4/6 inhibitor, Abemaciclib (LY2835219), and a cMET inhibitor, merestinib (LY2801653), were provided by Eli Lilly and Company, Indianapolis, IN, USA. Abemaciclib and merestinib were dissolved in dimethyl sulfoxide (DMSO) to a 10 mmol/L concentration and stored in small aliquots at −20 °C until further use. DMSO was used as solvent control. Recombinant Human HGF (rhHGF) was purchased from R & D systems, Minneapolis, MN, USA. Antibodies against cMET, phospho-cMET (T1234/1235), AKT, phospho-AKT (S473), ERK1/2, phospho-ERK1/2, RB, phospho-RB(S780), phospho-RB(S807/811), CDK4, cyclin A2, cyclin D1, p70 S6K, phospho-p70 S6K (T389), PARP, FOXM1 and β-actin were purchased from Cell Signaling Technology, Beverly, MA, USA. An antibody against Ki67 was purchased from DAKO, Cupertino, CA, USA.
Ohara M., Saito K., Kageyama K., Terai M., Cheng H., Aplin A.E, & Sato T. (2021). Dual Targeting of CDK4/6 and cMET in Metastatic Uveal Melanoma. Cancers, 13(5), 1104.
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2

Glioma Cell Line Cultivation and Isolation

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GBM cell lines were grown in DMEM supplemented with FBS (10%) and gentamycin (0.1 mg/mL). U87 cells were authenticated by short tandem repeat (STR) analysis in January 2016 (Genetica DNA Laboratories). The isolation of patient-derived glioma stem cells and generation of GSC lines (83Mes, MD30, and GBM43) has been previously described (8 (link), 22 (link)). GSCs were cultured in DMEM/F12 supplemented with EGF (20 ng/mL), bFGF (20 ng/mL), heparin (5 μg/mL), B27 (2%) and gentamycin (0.1 mg/mL). Merestinib was provided by Eli Lilly & Company and dissolved in DMSO for in vitro studies. For in vivo studies, Merestinib was first dissolved in PEG400 followed by sonication and addition of 20% captisol in water.
Bell J.B., Eckerdt F., Alley K., Magnusson L.P., Hussain H., Bi Y., Arslan A.D., Clymer J., Alvarez A.A., Goldman S., Cheng S.Y., Nakano I., Horbinski C., Davuluri R.V., James C.D, & Platanias L.C. (2016). MNK Inhibition Disrupts Mesenchymal Glioma Stem Cells and Prolongs Survival in a Mouse Model of Glioblastoma. Molecular cancer research : MCR, 14(10), 984-993.
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