Anti aldh2

The experimental procedure for Western blotting analysis that was used in this study was followed as described in our previous study [29 (link)]. The horseradish peroxidase-conjugated IgG that was anti-mouse and anti-rabbit (Thermo Fisher Scientific, New York, NY, USA) was used in this study. GAPDH was used as the control and for quantification. Antibodies purchased from Santa Cruz, USA: anti-EPCAM (1:1000, sc-25308), anti-ALDH1 (1:300, sc-374149), anti-ALDH2 (1:300, sc-100496), anti-KLF4 (1:1000, sc-166100), anti-GAPDH (1:1000, sc-47724). Antibodies purchased from Cell Signaling Technology, USA: anti-SNAI2 (#9585, 1:1000), anti-SOX2 (#3579, 1:1000), anti-OCT4 (#2840, 1:1000), anti-NANOG (#4903, 1:1000), anti-β-catenin (#8480, 1:1000), anti-c-Myc (#18583, 1:1500 dilution). Anti-PCNA (#60097-1-Ig, 1:5000) were purchased from Proteintech. The signal intensity was quantified using the protein imprinting imaging system (Tanon 5200, Better Tanon, Shanghai, China).

Western blot analysis was performed as described previously (Hosoi et al, 2010 (link)). The membranes were incubated with anti-phospho STAT3 (Tyr705: Cell Signaling; 1:1,000), anti-STAT3 (Santa Cruz, Santa Cruz, CA, USA; 1:1,000), anti-ALDH2 (Santa Cruz; 1:1000), and anti-ALDH1B1 (Santa Cruz; 1:1,000) antibodies, followed by an anti-horseradish peroxidase-linked antibody. Peroxidase was detected using an enhanced chemiluminescence system (GE Healthcare, Fairfield, CT, USA).