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Diarylpropionitrile

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Diarylpropionitrile is a chemical compound used in various research and laboratory applications. It serves as a core functional component in specific lab equipment and instrumentation.

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Lab products found in correlation

17β estradiol e2, by Merck Group (2 mentions) Propyl pyrazole triol, by Bio-Techne (2 mentions) Ici 182 780, by Bio-Techne (2 mentions) 4 2 phenyl 5 7 bis trifluoromethyl pyrazolo 1 5 a pyrimidin 3 yl phenol phtpp, by Bio-Techne (2 mentions) 5 fluorouracil 5 fu, by Merck Group (1 mentions) 4 2 phenyl 5 7 bis tri fluoro methyl pyrazolo 1 5 a pyrimidin 3 yl phenol, by Bio-Techne (1 mentions) Mouse anti β actin 60008 1 ig, by Proteintech (1 mentions) Ab92306, by Abcam (1 mentions) Ab27793, by Abcam (1 mentions) Ab32063, by Abcam (1 mentions) Zymosan, by Merck Group (1 mentions) Estradiol cypionate, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) 4 4 4 4 propyl 1h pyrazole 1 3 5 triyl trisphenol ppt, by Bio-Techne (1 mentions) Water soluble e2, by Merck Group (1 mentions) Cocaine hydrochloride, by Mallinckrodt (1 mentions) β cyano l alanine bca, by Cayman Chemical (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions)

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Diarylpropionitrile (DPN) (6 citations)

6 protocols using diarylpropionitrile

1

Estrogen Receptor Signaling Assay

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17β-estradiol (E2) (No. E2257), β-Estradiol 6-(O-carboxymethyl)oxime:BSA (BSA-E2) (No. E5630), 5-fluorouracil (5-FU) (No. 03738) were purchased from Sigma-Adrich and dissolved in 100% ethanol and PBS respectively. ERβ agonist Diarylpropionitrile (DPN) (No. 1494), ERα agonist propyl-pyrazole-triol (PPT) (No. 1426), ICI 182.780 (No. 1047) and ERβ antagonist 4-[2-phenyl-5,7-bis (tri-fluoro-methyl) pyrazolo [1,5-a] pyrimidin-3-yl] phenol (PHTPP) (No. 2662) were purchased from Tocris and dissolved in 100% ethanol. Transfection reagent FuGENE® HD (No. 4709705001) were purchased from Roche. The antibodies used are mouse anti-MLH1 (PAB11885, Abnova), mouse anti-ERα (ab32063, Abcam), mouse anti-ERβ (ab92306, Abcam), rabbit anti-c-Fos (ab27793, Abcam), mouse anti-β-actin (60008-1-Ig, Proteintech).
Lu J.Y., Jin P., Gao W., Wang D.Z, & Sheng J.Q. (2017). Estrogen enhances mismatch repair by induction of MLH1 expression via estrogen receptor-β. Oncotarget, 8(24), 38767-38779.
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2

Estrogen Receptor Agonists in Animal Study

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Propylpyrazoletriol (PPT) and diarylpropionitrile (DPN) were purchased from Tocris Bioscience (Minneapolis, MN, USA). Zymosan, estradiol cypionate (EC) and β-estradiol-water soluble were purchased from Sigma Chemical Company (St. Louis, MO, USA). Veterinary pentabiotic was purchased from Fort Dodge - Zoetis (São Paulo, SP, Brazil). estradiol cypionate, PPT and DPN were dissolved in corn oil while Zymosan and β-estradiol-water soluble were suspended and dissolved in sterile saline (0.9%), respectively.
Schneider A.H., Kanashiro A., Dutra S.G., do Nascimento de Souza R., Veras F.P., de Queiroz Cunha F., Ulloa L., Mecawi A.S., Reis L.C, & do Carmo Malvar D. (2019). Estradiol replacement therapy regulates innate immune response in ovariectomized arthritic mice. International immunopharmacology, 72, 504-510.
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3

Estrogen Receptor Signaling in Breast Cancer

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The human breast cancer MCF-7 and MDA-MB-231 cell lines were purchased from State Key Laboratory of Oncology in Southern China (Sun Yat-sen University Cancer Center, Guangzhou, People’s Republic of China). Cells were cultured in DMEM containing glucose (4.5 g/L; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS (Thermo Fisher Scientific) at 37°C in 5% CO2.
GEN and 17β-estradiol (E2) were obtained from Sigma-Aldrich (St Louis, MO, USA). The selective ERβ agonist diaryl propionitrile (DPN) and the selective ERβ antagonist 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]- pyrimidin-3-yl]phenol (PHTPP) were purchased from Tocris Bioscience (Bristol, UK).
Jiang H., Fan J., Cheng L., Hu P, & Liu R. (2018). The anticancer activity of genistein is increased in estrogen receptor beta 1-positive breast cancer cells. OncoTargets and therapy, 11, 8153-8163.
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4

Estrogen and Cocaine Modulation Study

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Water-soluble E2 (encapsulated in [2-Hydroxypropyl]-β-cyclodextrin) and (2-Hydroxypropyl)-β-cyclodextrin vehicle (VEH) were purchased from Sigma-Aldrich. E2 was dissolved in saline to a final concentration of 0.02 mg/mL and administered i.p. at a dose of 0.2 mg/kg according to Gresack et al (Gresack and Frick, 2006a (link), 2006b (link)). VEH-treated mice received the same amount of β-cyclodextrin as E2-treated mice. The ERβ agonist diarylpropionitrile (DPN) and the ERα agonist 4,4’,4’’-(4-Propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT) were purchased from Tocris. Solutions were prepared in sesame oil with 10% ethanol VEH to a final concentration of 0.5 mg/ml. Volumes of 50 μL were injected s.c. for a dose of ~1 mg/kg. Cocaine hydrochloride (Mallinckrodt Pharmaceuticals) was dissolved in saline and administered i.p. at a dose of 5 mg/kg.
Satta R., Certa B., He D, & Lasek A.W. (2017). Estrogen Receptor β in the Nucleus Accumbens Regulates the Rewarding Properties of Cocaine in Female Mice. International Journal of Neuropsychopharmacology, 21(4), 382-392.
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5

Antibody Reagents for Cell Signaling

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Antibodies used in this study were summarized in Table 1. ICI 182,780, 4,4,4-(4-propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT), diarylpropionitrile (DPN), 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy) phenol]-1Hpyrazoledihydrochloride (MPP), 4-[2-Phenyl-5,7-bis (trifluoromethyl)pyrazolo[1,5-a] pyrimidin-3yl]phenol (PHTPP) were from Tocris (Ellisville, MO). β-cyano-L-alanine (BCA) was from Cayman Chemical (Ann Arbor, MI). Estradiol-17β (E2β), fatty acid free bovine serum albumin (BSA), O-(carboxymethyl) hydroxylamine hemihydrochloride (CHH), fetal bovine serum (FBS), sodium dodecyl sulfate (SDS), hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES), and all other chemicals unless specified were from Sigma (St. Louis, MO).
Bai J., Lechuga T.J., Makhoul J., Yan H., Major C., Hameed A, & Chen D.B. (2023). ERα/ERβ-directed CBS Transcription Mediates E2β-stimulated hUAEC H2S Production. Journal of molecular endocrinology, 70(2), e220175.
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6

Signaling Pathway Profiling with Selective Estrogen Receptor Agonists

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E2, diarylpropionitrile (DPN), and propyl pyrazole triol (PPT) were purchased from Tocris (resuspended in ethanol) and used at a final concentration of 100 nM. PGE2 was purchased from Cayman, resuspended in ethanol, and used at a final concentration of 50–100 nM. Phosphatase Inhibitor Cocktail (cat # P5726) and Protease Inhibitor Cocktails (Cat # P8340) were purchased from Sigma (St. Louis, MO) and diluted 1:100 in the appropriate lysis buffer. The following antibodies were used for immunoblotting: SGK1 (Cell Signaling, Danvers, MA, Cat # 3272), cleaved PARP (Cell Signaling, Cat# 9542), pFOXO3a (Cell Signaling, Cat # 9464), FOXO3a (Cell Signaling, Cat# 2497), and β-actin (Sigma, Cat # A1978). Anti-SGK1 (Enzo, Farmingdale, NY, Cat# ADI-KAP-PK015-D) antibody was used for immunohistochemistry.
Monsivais D., Dyson M.T., Yin P., Navarro A., Coon J.S., Pavone M.E, & Bulun S.E. (2016). Estrogen receptor β regulates endometriotic cell survival through SGK1 activation. Fertility and sterility, 105(5), 1266-1273.
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