Adenosine diphosphate (adp)

All chemicals and reagents were of the highest purity commercially available. ATP was purchased from Roche Applied Science (Penzberg, Germany) and ADP was purchased from Bio Basic (Markham, ON, Canada). Nucleotide concentrations were determined by absorbance at 259 nm using ε₂₅₉ of 15,400 M⁻¹ cm⁻¹. In all experiments one molar equivalent of MgCl₂ was added to nucleotide solutions immediately before use.
3-(N-Morpholino)propanesulfonic acid (MOPS), Ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA), Apyrase (potato grade VII), phalloidin were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Alternatively, the same form of phalloidin was purchased also from Setareh Biotech (Eugene, OR, USA). MgCl₂∙6H₂O came from Bio Basic (Markham, ON, Canada) and KCl from Merk (Darmstadt, Germany).

All chemicals and reagents were of the highest purity commercially available. ATP was purchased from Roche Applied Science (Penzberg, Germany) and ADP was purchased from Bio Basic (Markham, ON, Canada). Nucleotide concentrations were determined by absorbance at 259 nm using ε₂₅₉ of 15,400 M⁻¹ cm⁻¹. The N-methylanthraniloyl (mant) derivatives of the nucleotides, 3′-mant-2′-deoxy-ATP (mT), 2′/3′-mantADP (mD) were purchased from Jena Bioscience (Jena, Germany) and their concentrations was determined by absorbance at 259 nm using ε₂₅₅ of 23,300 M⁻¹ cm⁻¹. In all experiments one molar equivalent of MgCl₂ was added to nucleotide solutions immediately before use. N-(1-pyrene)iodoacetamide came from Molecular Probes (Eugene, OR, USA). 7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin, 3-(N-Morpholino)propanesulfonic acid (MOPS), Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), Apyrase (potato grade VII), 7-Methylguanosine 5′-diphosphate sodium salt (7-MEG), purine-nucleoside phosphorylase (PNP, bacterial) and phalloidin were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Alternatively, the same form of phalloidin was purchased also from Setareh Biotech (Eugene, OR, USA). MgCl₂∙6H₂O came from Bio Basic (Markham, ON, Canada) and KCl from Merk (Darmstadt, Germany).