Rpmi 1640

Manufactured by Microgem

Sourced in United States, Italy

RPMI 1640 is a cell culture medium formulated to support the growth of various cell types, including suspension and adherent cells. It provides a balanced salt solution, amino acids, vitamins, and other essential components required for cell maintenance and proliferation.

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Lab products found in correlation

Beacon designer 3, by Bio-Rad (2 mentions) Custom primers, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Microgem (1 mentions) Blasticidin, by Thermo Fisher Scientific (1 mentions) Renca, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Euroclone (1 mentions) Renca, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Histopaque 1077, by GE Healthcare (1 mentions) L glutamine, by Merck Group (1 mentions) Amphotericin b, by Lonza (1 mentions) Antibiotic antimycotic solution, by Lonza (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Lipopolysaccharides (lps), by Lonza (1 mentions)

3 protocols using rpmi 1640

Establishment and Maintenance of Renal Adenocarcinoma and Fibrosarcoma Cell Lines

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Renal adenocarcinoma cell line Renca was purchased from the American Type Culture Collection (ATCC). Tumor cells were maintained in RPMI-1640 (Microgem), supplemented with 10% fetal bovine serum (FBS; Microgem). The Renca-Luc variant was obtained by infecting Renca cells with a lentiviral vector carrying the coding sequence of synthetic firefly luciferase gene luc2 and using blasticidin (5 µg mL⁻¹; Gibco) as a selection antibiotic. The Renca-iRFP670 is the fluorescent Renca variant stably expressing the iRFP670 fluorescent protein. The UV-2237 fibrosarcoma cell line^{26 (link)} was maintained in DMEM (EuroClone) supplemented with 10% FBS. Stocks of the cell lines were stored frozen in liquid nitrogen and used within four weeks after thawing. Cells were routinely tested by PCR for Mycoplasma contamination.
The Lewis Lung carcinoma cell line (LLC) was obtained from the Developmental Therapeutics Program (DTP) – Division of Cancer Treatment and Diagnosis (DCTD) Tumor Repository, NCI, US. LLC was stocked frozen as in vivo-derived tumor fragments and maintained subcutaneously in C57/BL6 mice before testing.

Russo M., Panini N., Fabbrizio P., Formenti L., Becchetti R., Matteo C., Meroni M., Nastasi C., Cappelleri A., Frapolli R., Nardo G., Scanziani E., Ponzetta A., Bani M.R., Ghilardi C, & Giavazzi R. (2023). Chemotherapy-induced neutropenia elicits metastasis formation in mice by promoting proliferation of disseminated tumor cells. Oncoimmunology, 12(1), 2239035.

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Immune Response Modulation Protocol

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The following were used for the experiments: l-glutamine (Sigma-Aldrich^® 155 St. Louis, MO, USA), antibiotic–antimycotic solution (10.000 IU/ml penicillin, 10 mg/ml streptomycin, and 25 µg/ml amphotericin B) (Lonza, Basel, CH), MEM non-essential amino acids (Microgem^®, Naples, Italy), fetal bovine serum (FBS) (Sigma-Aldrich® 155 St. Louis, MO, USA), RPMI 1640 (Microgem^®, Naples, Italy), Histopaque-1077 (GE Healthcare Bio-Sciences AB SE-751 84 Uppsala, Sweden), detoxified LPS (from Escherichia coli serotype 026:B6) (Sigma-Aldrich^® 155 St. Louis, MO, USA), 1,2-Bis (diphenylphosphino)ethane [DPPE]-polyethylene glycolmonomethylether [PEG] was from Avanti Polar Lipids (Alabaster, AL). Simplexide was isolated from Plakortis simplex at the Department of Pharmacy, University of Naples Federico II. Target-specific primers for CXCL8, IL6, TNFα, and GAPDH were designed using the Beacon Designer 3.0 (Biorad Laboratories, Milan, Italy) and produced and purified by Custom Primers (Life Technologies, Milan, Italy).

Marcella S., Afoullouss S., Thomas O.P., Allcock A.L., Murphy P.V, & Loffredo S. (2021). Immunomodulatory properties of characellide A on human peripheral blood mononuclear cells. Inflammopharmacology, 29(4), 1201-1210.

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Immune Cell Isolation and Characterization

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The following reagents were purchased: L-glutamine, fetal bovine serum (FBS), LPS (from Escherichia coli serotype 026:B6), Percoll^®, piperazine- N, N_-bis-2-ethanesulphonic acid (PIPES), phosphate buffer saline (PBS), Triton X-100, antibiotic–antimycotic solution (10,000 IU/mL penicillin, 10 mg/mL streptomycin and 25 µg/mL amphotericin B) (Lonza, Basel, CH), RPMI 1640 (Microgem, Naples, Italy). Target-specific primers for CXCL8, IL-6, TNFα, IL-1β, ACE2, TMPRSS2, and GAPDH were designed using the Beacon Designer 3.0 (Bio-Rad Laboratories, Milan, Italy) and produced and purified by Custom Primers (Life Technologies, Milan, Italy). DMSO (Merck Millipore, Burlington, MA, USA). Polyclonal antibody to human TLR2 (anti-TLR2) (InvivoGen, Rho, Italy).

Palestra F., Poto R., Ciardi R., Opromolla G., Secondo A., Tedeschi V., Ferrara A.L., Di Crescenzo R.M., Galdiero M.R., Cristinziano L., Modestino L., Marone G., Fiorelli A., Varricchi G, & Loffredo S. (2023). SARS-CoV-2 Spike Protein Activates Human Lung Macrophages. International Journal of Molecular Sciences, 24(3), 3036.

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