Cleaved and total caspase 3

Manufactured by Cell Signaling Technology

Cleaved and total caspase 3 is a lab equipment product that detects and quantifies the levels of both cleaved and total caspase 3 protein. Caspase 3 is a key executioner protein involved in the apoptosis (programmed cell death) pathway.

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Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Odyssey ir imager, by LI COR (1 mentions) Lkb1 m 18, by Santa Cruz Biotechnology (1 mentions) Phospho ampk thr172, by Cell Signaling Technology (1 mentions) Gapdh, by Abcam (1 mentions) Bradford method, by Bio-Rad (1 mentions) Multi gauge software, by Fujifilm (1 mentions) Acetylated lysine, by Cell Signaling Technology (1 mentions) Suramin, by Merck Group (1 mentions) Anti flag m2 magnetic bead, by Merck Group (1 mentions) Aicar, by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Cell culture reagents, by GE Healthcare (1 mentions) N6022, by Merck Group (1 mentions) 3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2 h tetrazolium bromide mtt, by Sangon (1 mentions) Attractene, by Thermo Fisher Scientific (1 mentions) Fluor de lys sirt1 fluorometric drug discovery assay kit, by Enzo Life Sciences (1 mentions) Muse count viability assay kit, by Merck Group (1 mentions) Protein a magnetic bead, by Merck Group (1 mentions) Foxo1, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Cleaved caspase-3 (3596 citations) Caspase-3 (2138 citations) Total caspase-3 (31 citations) Caspase-3 and cleaved caspase-3 (7 citations) Caspases (2 citations)

4 protocols using cleaved and total caspase 3

Whole-Heart Protein Lysate Analyses

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Whole‐heart protein lysates made from freeze‐clamped hearts using lysis buffer (HEPES pH 7.4 [20 mmol/L], β‐glycerol phosphate [50 mmol/L], EGTA [2 mmol/L], DTT [1 mmol/L], NaF [10 mmol/L], NaVO4 [1 mmol/L], Triton X‐100 [1%], glycerol [10%,] and 1 protease inhibitor complete mini tablet‐EDTA free/20 mL [Roche]) were subjected to SDS‐PAGE with protein content quantified using the Bradford method (Bio‐Rad). Following transfer, nitrocellulose membranes were probed with primary antibodies (LKB1 [D60C5; Cell Signaling]), LKB1 (M‐18; Santa Cruz Biotechnology), phospho‐LKB1 (Ser428/431; Cell Signaling), phospho‐AMPK (Thr172; Cell Signaling), total AMPK α2 (Santa Cruz Biotechnology), GAPDH (Abcam), STRAD (S‐17; Santa Cruz Biotechnology), MO25 (Cell Signaling), VDAC (Abcam), PUMA (Cell Signaling), SNRK (Sigma‐Aldrich), cleaved and total caspase 3 (Cell Signaling), and visualized using LI‐COR secondary antibodies and the LI‐COR Odyssey IR imager. Densitometry was quantified using Odyssey software. Primary antibody directed against p53 (Cell Signaling) was detected using enhanced chemiluminescence and quantified using MultiGauge software (Fujifilm).

Miller E.J., Calamaras T., Elezaby A., Sverdlov A., Qin F., Luptak I., Wang K., Sun X., Vijay A., Croteau D., Bachschmid M., Cohen R.A., Walsh K, & Colucci W.S. (2015). Partial Liver Kinase B1 (LKB1) Deficiency Promotes Diastolic Dysfunction, De Novo Systolic Dysfunction, Apoptosis, and Mitochondrial Dysfunction With Dietary Metabolic Challenge. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 5(1), e002277.

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AMPK-p53 Signaling Modulation in Cell Assays

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Cell culture reagents were purchased from GE and fetal bovine serum (FBS) was purchased from PAN-Biotech; DTT was purchased from Millipore (Massachusetts, USA); Compound C, AICAR, Resveratrol, Suramin, SNAP, and ODQ were purchased from Sigma-Aldrich; N6022, Pifithrin-α, VO-Ohpic trihydrate, AS1842856, and MK 2206 were from MCE. Hydrogen peroxide (H₂O₂) was from Sinopharm Chemical Reagent; 3-(4,5-dimethyl-2-thiazolyl)−2,5-diphenyl-2-H-tetrazolium bromide (MTT) was purchased from Sangon Biotech; Lipofectamine® 3000 and Attractene were from Thermo Fisher Scientific and Qiagen, respectively. Fluor-de-Lys SIRT1 fluorometric drug discovery assay kit was from Enzo Life Sciences; Hydrogen Peroxide Assay Kit, Nitric Oxide Assay Kit, RIPA lysis buffer, Bicinchoninic Acid assay Kit, and Nuclear and Cytoplasmic Protein Extraction Kit were from Beyotime; Super ECL Prime was from US Everbright®Inc. The mRNA extraction kit was from Bioteke. Protein A Magnetic Beads and the Muse Count & Viability Assay Kit were purchased from Millipore. Anti-Flag M2 Magnetic Beads was from Sigma-Aldrich. Antibodies for phospho- and total-AMPK, acetyl- and total-p53, SIRT1, Cleaved- and total-Caspase-3, FOXO1, PTEN, β-actin, and acetylated-Lysine were from Cell Signaling Technology; antibodies for P21 was from Abcam; antibodies for Prdx2 was from Santa Cruz Biotechnology.

Zhang Y., Sun C., Xiao G., Shan H., Tang L., Yi Y., Yu W, & Gu Y. (2019). S-nitrosylation of the Peroxiredoxin-2 promotes S-nitrosoglutathione-mediated lung cancer cells apoptosis via AMPK-SIRT1 pathway. Cell Death & Disease, 10(5), 329.

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Antibody Panel for Cell Signaling

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eIF2α, P-eIF2α (Ser51), Total S6K1, P-S6K1 (T389), Cleaved PARP, total and cleaved caspase-3 antibodies were purchased from Cell Signaling Technologies, ATF4 (SC-200) from Santa Cruz and MAP1LC3B antibody from Novus. Antibody Puromycin (clone 12D10) was kindly given by Phillipe Pierre (Centre d'Immunologie de Marseille-Luminy, France).

Mesclon F., Lambert-Langlais S., Carraro V., Parry L., Hainault I., Jousse C., Maurin A.C., Bruhat A., Fafournoux P, & Averous J. (2017). Decreased ATF4 expression as a mechanism of acquired resistance to long-term amino acid limitation in cancer cells. Oncotarget, 8(16), 27440-27453.

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Islet Protein Quantification by Western Blot

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Freshly isolated islets from 3–4 month old male mice were sonicated in 150–200 μl lysis buffer supplemented with protease inhibitor tablet (Roche Diagnostics, Basel, Switzerland). The cell extract was diluted by 1–1.5 volume Laemmli loading buffer containing 5% β-mercaptoethanol (Sigma-Aldrich, St. Louis, MO) and boiled for 5 min before loading onto SDS-PAGE gels. Together with liver and visceral fat extracts, Western blotting was performed to quantify protein levels of 11β-HSD1 (1:250, H-10 sc-20175, Santa Cruz, Dallas, TX) and β-actin (Santa Cruz or Medimabs, Montreal, QC). Similarly, MIN6 cells were used to probe for total and cleaved caspase-3 (1:1000, Cell Signaling, Danvers, MA).

Chowdhury S., Grimm L., Gong Y.J., Wang B., Li B., Srikant C.B., Gao Z.H, & Liu J.L. (2015). Decreased 11β-Hydroxysteroid Dehydrogenase 1 Level and Activity in Murine Pancreatic Islets Caused by Insulin-Like Growth Factor I Overexpression. PLoS ONE, 10(8), e0136656.

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