Pure reference standard of nirmatrelvir (99.36%) and ritonavir (99.62%) were kindly supplied by Pfizer, Inc, Egypt. The purity was checked by applying the reported method [13 ].
Paxlovid tablets, pink oval nirmatrelvir film-coated tablet (150 mg) co-packaged with white ritonavir film-coated tablet (100 mg), (B. NO: A1324, manufactured by Pfizer Company), were kindly supplied by Pfizer, Inc., Egypt. The recommended dose is two tablets of nirmatrelvir plus one tablet of ritonavir.
Acetonitrile, chloroform, ethanol and methanol (Sigma-Aldrich, Germany).
Tween 80, Sodium dodecyl sulphate “SDS”, El-Nasr Company, Egypt. Cetyl trimethyl ammonium bromide “CTAB” (Win lab, UK). β-cyclodextrin “β-CD”, Sigma-Aldrich, Germany.
Hydrochloric acid and Sodium hydroxide, El-Nasr Company, Egypt. 0.1 N aqueous solutions were prepared.
Boric acid, glacial acetic acid, potassium chloride acid and sodium acetate trihydrate, El-Nasr Company, Egypt.
Acetate buffer solutions in the pH range 4 to 6 and alkaline borate buffer in the pH range 8 to 10.
Drug-free human plasma of healthy volunteers was supplied by Blood Bank, Al-Azhar University Hospital, Damietta, Egypt.