Corn fiber was provided by Samyang Genex Co. (Seoul, Korea). On a dry solid basis, the corn fiber used in this investigation had a starch content of 153.6 g/kg, a protein content of 105.2 g/kg, a fat content of 42.4 g/kg, and an ash content of 51.5 g/kg. In addition, the raw corn fiber had a soluble fiber content of 12.3 g/kg, an insoluble fiber content of 617.3 g/kg, and a total dietary fiber content of 629.4 g/kg. Cellulase was purchased from Novozymes Co. (Bagsvared, Denmark). Termamyl (i.e., thermostable α-amylase) and the xylose standard were purchased from Sigma Chemical Co. (Steinheim, Germany).
Thermostable α amylase
Manufactured by Merck Group
Sourced in United States
Thermostable α-amylase is a laboratory enzyme that catalyzes the hydrolysis of starch into smaller maltodextrin units. It is derived from a thermophilic microorganism and is stable at high temperatures, making it suitable for use in various industrial and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Termamyl, by Merck Group (1 mentions)
Cellulase, by Novozymes (1 mentions)
Xylose standard, by Merck Group (1 mentions)
Folin ciocalteu, by Merck Group (1 mentions)
Amyloglucosidase, by Merck Group (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
β carotene, by Merck Group (1 mentions)
Abts 2 2 azinobis 3 ethylbenzothiazoline 6 sulphonic acid, by Merck Group (1 mentions)
Concentrated hcl, by Thermo Fisher Scientific (1 mentions)
Laccase from trametes versicolor, by Merck Group (1 mentions)
Hexane, by Thermo Fisher Scientific (1 mentions)
Ethanol, by Thermo Fisher Scientific (1 mentions)
Methanolic hcl, by Merck Group (1 mentions)
Dinitrosalicylic acid dns, by Merck Group (1 mentions)
Endo arabinanase, by Megazyme (1 mentions)
Amyloglucosidase, by Megazyme (1 mentions)
5 protocols using thermostable α amylase
1
Characterization of Corn Fiber Composition
2
Comprehensive Phytochemical Analysis
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Trolox, caffeic acid, 3- and 4-caffeoylquinic acids, 3,4-, 3,5- and 4,5- dicaffeoylquinic acids, and β-carotene (≥97.0 %, UV) standards, thermostable α-amylase, amyloglucosidase, protease, Folin-Ciocalteu, ABTS (2,2′-azinobis-(3-ethylbenzothiazoline-6- sulphonic acid)) and DPPH (2,2-diphenyl-1-picrylhydazyl) reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA). Cyanidin-3-glucoside and chlorogenic acid standards were purchased from Fluka (St. Louis, MO, USA). Acetonitrile, methanol, ethylacetate, triethylamine and acetic acid were HPLC grade. The rest of the chemicals were of analytical grade.
3
Corn Bran Valorization and Pectin Analysis
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Dry milled corn bran was gifted from Agricor, Ltd. (Marion, IN, USA). A commercial sample of low methoxy pectin from the citrus peel (900-69-5) and fructooligosaccharide (FOS—F8052) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Thermostable α-amylase, laccase from Trametes versicolor, and methanolic-HCl were obtained from Sigma-Aldrich. Hexane, sodium hydroxide pellet, ethanol, concentrated HCl, and Tri-Sil were purchased from Fisher Scientific (Thermo Fisher Scientific, Suwanee, GA, USA).
4
Characterization of Native Corn Starch
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The native corn starch with 27.9% amylose was obtained from a large supermarket (Xi’an Guowei Starch Company, Xi’an, China). Its moisture content and protein content were 10.7% and ≤0.2%, respectively. Thermostable α-amylase (1400 U/mg) from porcine pancreas (type VI-B), pullulanase (1000 U/mL) and dinitrosalicylic acid (DNS) were purchased from Sigma–Aldrich Chemical Co. (St. Louis, MO, USA). A Megazyme Resistant Starch Assay Kit was purchased from Megazyme Int. Ireland Ltd., Co. (Wicklow, Ireland). All other reagents were of analytical grade.
5
Enzymatic Hydrolysis of Polysaccharides
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If not stated otherwise, all chemicals used were of “p.a.” grade or better and were purchased from VWR (Radnor, PA, United States), Sigma-Aldrich (Schnelldorf, Germany), or Carl Roth (Karlsruhe, Germany). Thermostable α-amylase (EC 3.2.1.1, from Bacillus licheniformis, 20,000–60,000 U/mL) was purchased from Sigma. Protease (EC 3.4.21.14, subtilisin A from B. licheniformis, 300 U/mL), amyloglucosidase (EC 3.2.1.3, from Aspergillus niger, 200 U/mL), endo-arabinanase (EC 3.2.1.99, from A. niger, 9 U/mg), and endo-galactanase (EC 3.2.1.89, from A. niger, 408 U/mg) were purchased from Megazyme (Bray, Ireland).
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