D 6072

Manufactured by Nabertherm

Sourced in Germany

The D-6072 is a laboratory equipment offered by Nabertherm. It is designed for specialized applications in controlled environments. The core function of this product is to provide precise temperature regulation and monitoring capabilities for research and testing purposes. No further details or interpretations are provided.

Automatically generated - may contain errors

Lab products found in correlation

Muffle furnace, by Nabertherm (2 mentions) Aa 6880f, by Shimadzu (1 mentions) Whatman filter paper, by Cytiva (1 mentions)

4 protocols using d 6072

Determination of Mineral Content in Anchote Tuber

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the determination of some minerals (Ca, Mg, Fe, Zn, and Cu), a 1.0 g anchote tuber flour was calcinated in a muffle furnace (Nabertherm, D‐6072 Dreieich, Germany) at 450°C for 4 hr. The ash was dissolved in 20% nitric acid and filtered using the Whatman filter paper (42 mm) before analysis. Then, the minerals Ca, Mg, Fe, Zn, and Cu were determined using atomic absorption spectrophotometer (SHIMADZU, AA‐6880F, Japan) using air/acetylene flame at wavelengths of 422.7, 285.2, 248.3, 213.9, and 324.7 nm respectively. Finally, the concentrations of the elements in the samples were calculated using the following equation.

Concentration (mg / 100 g) = \frac{(a ‐ b) x V}{10 x W s}

where a = concentration in ppm (mg/L) of sample; b = concentration in ppm (mg/L) of blank solution, V = Volume in ml of extract, Ws = Weight of sample in g, 10 = conversion factor.

Bikila A.M., Tola Y., Esho T.B, & Forsido S.F. (2020). Effect of predrying treatment and drying temperature on proximate composition, mineral contents, and thermophysical properties of anchote (Coccinia abyssinica (Lam.) Cogn.) flour. Food Science & Nutrition, 8(10), 5532-5544.

+ Open protocol

+ Expand

Determination of Ash Content in Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ash was determined by igniting the samples in a muffle furnace according to Ref. [19 ] method 923.03. Washed and dried crucibles were weighed correctly (W₁). Briefly, 2 g of the sample was weighed and placed on each crucible (W₃). The samples were burned and ignited in a muffle furnace (Nabertherm, D-6072 Dreieich, Germany) at 550 °C for 4 h until the grey-white color of the sample developed. Then the sample was cooled in a desiccator for about 1 h and immediately weighed (W₂). Ash was then determined using in Equation (2).

Equation 2.

Where: W₁ is the weight of the crucible, W₂ is the weight of the sample and crucible after drying, and W₃ is the weight of the sample in dry weight basis (db).

Yimer A., Forsido S.F., Addis G, & Ayelign A. (2023). Nutritional composition of some wild edible plants consumed in Southwest Ethiopia. Heliyon, 9(6), e16541.

+ Open protocol

+ Expand

Determination of Crude Fiber Content

Check if the same lab product or an alternative is used in the 5 most similar protocols

Crude fiber content was determined by the Weende scheme as adopted by Bekele and Bekele (2018). About one gram of the dried sample was boiled for 40 min in 50 ml dilute sulfuric acid (2.5%) and filtered. The residue then washed with distilled water and again boiled in 50 ml of 2.5% sodium hydroxide for 40 min. The residue consecutively washed with 20 ml ethanol (99.8%) and 20 ml diethyl ether two times, followed by washing with 20 ml acetone three times. The insoluble residue consisted of crude fiber, and ash was dried in oven (Blast Air Oven, DHG‐9240A, China) and weighed (w₁). This residue was burned in a furnace (Nabertherm, D‐6072 Dreieich, Germany) at 550°C for 3 hr (w₂) and the weight difference (w₁‐w₂) taken as crude fiber.

+ Open protocol

+ Expand

Crude Fiber Content Determination Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Crude fiber content was determined based on AOAC (2000) official method 962.09. One gram of sample (W₁) was boiled for 40 min in 50 ml dilute sulfuric acid (2.5 %) and then, the acid was drained using a vacuum pump. After washing with distilled water, the residue was boiled for 40 min in 50 ml of 2.5 % sodium hydroxide. After that, it was washed with once 20 ml of 99.8 % ethanol, twice with 20 ml of diethyl ether, and three times with 20 ml of acetone. The insoluble residue (crude fiber and ash) was dried in an oven (Blast Air Oven, DHG-9240A, China) and weighed (W₂). This residue was burned at 550 °C for 3 h (W₃) in a furnace (Nabertherm, D-6072 Dreieich, Germany), and the crude fiber percentage was calculated using Eq. (5).

Equation 5.

where; W₁ - the weight of the sample, W₂ - the weight of dried insoluble residue (crude fiber + ash), and W₃ - the weight of burned residue (ash).

Alemayehu G.F., Forsido S.F., Tola Y.B., Teshager M.A., Assegie A.A, & Amare E. (2021). Proximate, mineral and anti-nutrient compositions of oat grains (Avena sativa) cultivated in Ethiopia: implications for nutrition and mineral bioavailability. Heliyon, 7(8), e07722.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!