The rhizome of Polygonatum sibiricum (Origin: Hebei, China) was supplied by Changsha central hospital (Hunan, China). Lipopolysaccharide (LPS) and thiazolyl blue (MTT) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). LDH and nitric oxide commercial kits were obtained from Nanjing Jiancheng Bio-engineering Institute (Nanjing, China). Cytokine detecting ELISA kits were purchased from Shanghai Westang Bio-Tech Co., Ltd. (Shanghai, China). Specific primers (IL-6, TNF-α, GAPDH) were purchased from Biosune Biotechnology Co., Ltd. (Shanghai, China). All the other chemicals used were of analytical grade.
Thiazolyl blue
Manufactured by Solarbio
Sourced in China
Thiazolyl blue is a tetrazolium compound commonly used in colorimetric assays to measure cell viability, proliferation, and cytotoxicity. It is a yellow solid that is reduced by cellular enzymes to form a dark blue formazan product, which can be quantified spectrophotometrically.
Automatically generated - may contain errors
2 protocols using thiazolyl blue
1
Polygonatum sibiricum Rhizome Anti-inflammatory Effects
2
Assessing Cell Viability and Colony Formation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was detected by Thiazolyl Blue Tetrazolium Bromide (MTT) assay (Beijing Solarbio Science & Technology).
For colony formation assay, clonogenic growth was determined by plating 1 × 104 cells in 0.5 mL of 0.33% agar/RPMI 1640 supplemented with 10% FBS. Medium was supplied twice weekly, and cells were cultured for around 2 weeks. Clusters of cells were considered to be a clonogenic colony if over 40 cells. Then the colonies were imaged, and the numbers were counted by using ImageJ.
For cell cycle assay, MM cells were fixed by 70% ethanol, washed with PBS and treated with propidium iodide (PI) solution (Yeasen, China) for 30 min. The samples were analyzed by flow cytometry (Merck Millipore, Germany).
For colony formation assay, clonogenic growth was determined by plating 1 × 104 cells in 0.5 mL of 0.33% agar/RPMI 1640 supplemented with 10% FBS. Medium was supplied twice weekly, and cells were cultured for around 2 weeks. Clusters of cells were considered to be a clonogenic colony if over 40 cells. Then the colonies were imaged, and the numbers were counted by using ImageJ.
For cell cycle assay, MM cells were fixed by 70% ethanol, washed with PBS and treated with propidium iodide (PI) solution (Yeasen, China) for 30 min. The samples were analyzed by flow cytometry (Merck Millipore, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!