Avance neo 400 mhz nmr spectrometer

Manufactured by Bruker

Sourced in United States

The Avance Neo 400 MHz NMR spectrometer is a high-performance nuclear magnetic resonance (NMR) instrument designed for analytical and research applications. It operates at a frequency of 400 MHz and is capable of performing various NMR spectroscopy techniques to analyze the chemical structure and composition of samples.

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Lab products found in correlation

Ft ir 1760x spectrophotometer, by PerkinElmer (3 mentions) Microtof mass spectrometer, by Bruker (2 mentions) Irtracer 100 infrared spectrometer, by Shimadzu (1 mentions) Tga550, by Mettler Toledo (1 mentions) Lc 20ad hplc system, by Shimadzu (1 mentions) C18 column, by Shimadzu (1 mentions) Uv detector, by Shimadzu (1 mentions) Fluoromax 4 spectrometer, by Horiba (1 mentions) Rp 18, by Merck Group (1 mentions) High performance liquid chromatography (hplc), by Shimadzu (1 mentions) Silica gel 60, by Merck Group (1 mentions) Silica gel 60 f254 plate, by Merck Group (1 mentions) Sephadex lh 20, by Merck Group (1 mentions) Spectronic 3000 array spectrophotometer, by Milton Roy (1 mentions) P 2000 digital polarimeter, by Jasco (1 mentions) J 810, by Jasco (1 mentions) Kieselgel 60, by Merck Group (1 mentions) Lct premier time of flight mass spectrometer, by Waters Corporation (1 mentions) Lct premier, by Waters Corporation (1 mentions) Unity plus 400 mhz spectrometer, by Bruker (1 mentions)

Close spelling variants of this product

Avance NEO 400 NMR spectrometer AVANCE NEO 400 MHz spectrometer Avance 400 MHz NMR spectrometer Avance Neo 400 MHz Avance Neo NMR spectrometer Avance Neo 400 spectrometer NEO 400 MHz spectrometer Avance 400 NMR spectrometer Avance 400 MHz spectrometer Avance 400 (400 MHz) spectrometer

9 protocols using avance neo 400 mhz nmr spectrometer

Structural and Thermal Characterization of LDMS

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The synthetic product was characterized by the potassium bromide (KBr) tabletting method using an IRTRacer-100 infrared spectrometer (Shimadzu Corporation, Kyoto, Japan). A small amount of sample was mixed well with KBr in a mortar and ground thoroughly, and the ground powder was pressed into a complete and transparent sheet using a tablet press. The scanning wavelength range was from 500 to 4000 cm⁻¹, the resolution ratio was 4 cm⁻¹, and the sample scanning times were 32.
To further characterize the molecular structure of LDMS, its NMR hydrogen spectrum was measured using an Avance NEO 400 MHz NMR spectrometer (Bruker, Berlin, Germany). The signal was collected by dissolving 3 mg of sample in heavy water and cycling 16 times.
The thermal stability of LDMS was measured using a thermal analyzer (TGA550, Mettler Toledo Co. Shanghai, China) in a N₂ environment. The experimental temperature range was 50.0 to 800.0 °C, and the heating rate was 10.0 °C/min.
The relative molecular mass distribution of the filtrate reducer samples LDMS and Driscal was determined by gel chromatography using a Waters 1515 GPC type gel chromatograph (Waters Corporation, Milford, MA, USA). The mobile phase of the test was 0.1 mol/L aqueous sodium nitrate solution, and the standard was polyethylene glycol (PEG).

Dong X., Sun J., Huang X., Li J., Lv K, & Zhang P. (2022). Synthesis of a Low-Molecular-Weight Filtrate Reducer and Its Mechanism for Improving High Temperature Resistance of Water-Based Drilling Fluid Gel System. Gels, 8(10), 619.

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Oxidation Product Identification of BH and PAH

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For identification of the oxidation product of BH, reaction mixtures of BH with [IrCl₆]²⁻ were analyzed by reversed-phase high performance liquid chromatography (RP-HPLC) using a Shimadzu LC-20 AD HPLC system equipped with a UV detector (Shimadzu Corporation, Kyoto, Japan). A C18 column of Shimadzu (250 × 4.6 mm, 5 µm in particle size) and an injection loop of 20 µL were used for sample separations and injections. Moreover, the injection loop was always fully filled with samples. After optimizations of mobile phase in an isocratic elution mode, a solvent mixture of H₂O:MeOH = 4:1 (v/v) was chosen as the mobile phase. The UV detector was set at 261 nm and the flow rate was at 1.0 mL/min. Under the optimized conditions, a reaction mixture containing 2.5 mM BH and 2.5 mM [IrCl₆]²⁻ in a phosphate buffer of pH 6.34 after a reaction time of 10 min was subjected to analysis.
In the case of PAH, ¹H-NMR spectroscopy (AVANCE NEO 400 MHz NMR spectrometer, Bruker, Switzerland) was utilized to analyze the oxidation product. Two samples were prepared for NMR experiments: (a) 1 mM PAH in D₂O which contained 0.02% TSP and (b) a reaction mixture of 1 mM PAH and 5 mM [IrCl₆]²⁻ after a reaction time of about 5 hrs.

, & Zhang X. (2020). Oxidations of Benzhydrazide and Phenylacetic Hydrazide by Hexachloroiridate(IV): Reaction Mechanism and Structure–Reactivity Relationship. Molecules, 25(2), 308.

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Spectroscopic Analysis of Compounds 6a and 6b

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The ¹H and ¹³C NMR spectra were conducted with Bruker Avance NEO 400 MHz NMR spectrometer using DMSO-d₆ as the solvent. The UV-vis absorption and fluorescence emission spectra were recorded on Shimadzu UV-2550 spectrometer and Horiba FluoroMax®-4 spectrometer, respectively. Emission spectra of 6a and 6b were observed for the excitation wavelengths of 470 nm and 475 nm, respectively. Micro fluorometer cuvettes (1 mm light path, 0.35 mL volume) were used to avoid reabsorption of emission from the sample.

Chen H.Y., Yao C.C., Tseng T.Y., Yeh Y.C., Huang H.S, & Yeh M.Y. (2021). Synthesis and photophysical properties of benzoxazolyl-imidazole and benzothiazolyl-imidazole conjugates. RSC Advances, 11(63), 40228-40234.

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Analytical Techniques for Compound Characterization

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Optical rotation was
determined by a Jasco P-2000 digital polarimeter (Easton, MD, USA).
UV spectra were recorded with a Milton Roy Spectronic 3000 Array spectrophotometer
(Rochester, Monroe, NY, USA). CD spectra were obtained from a Jasco
J-815 CD spectrophotometer (Hachioji, Tokyo, Japan). FT-IR spectra
were measured with a PerkinElmer FT-IR 1760X spectrophotometer (Boston,
MA, USA). The Bruker MicroTOF mass spectrometer (ESI-MS) (Billerica,
MA, USA) was used to measure mass spectra. NMR spectra were carried
through a Bruker Avance Neo 400 MHz NMR spectrometer (Billerica, MA,
USA). Semipreparative high-performance liquid chromatography (HPLC)
was conducted using the Shimadzu HPLC (Kyoto, Japan). Chromatography
was conducted by silica gel 60 (no. 1.07734.2500), size 0.063–0.200
mm and (no. 1.09385.2500), size 0.040–0.063 mm (Merck, NJ,
USA). Reverse-phase column chromatography was done by RP-18 (no. 1.13900.1000),
size 40–63 μm (Merck KGaA, Darmstadt, Germany). Sephadex
LH-20 (Merck, NJ, USA) was also used for the isolation of compounds.
Diaion HP-20 column chromatography was performed on polar copolymer
styrene-divinylbenzene adsorbent resin with a particle size of 0.5
mm in diameter (Mitsubishi, Tokyo, Japan). The purity of isolated
compounds was initially assessed through thin-layer chromatography,
silica gel 60 F₂₅₄ plates (Merck, NJ, USA) under UV light.

Thant M.T., Bhummaphan N., Wuttiin J., Puttipanyalears C., Chaichompoo W., Rojsitthisak P., Punpreuk Y., Böttcher C., Likhitwitayawuid K, & Sritularak B. (2024). New Phenolic Glycosides from Coelogyne fuscescens Lindl. var. brunnea and Their Cytotoxicity against Human Breast Cancer Cells. ACS Omega, 9(7), 7679-7691.

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Comprehensive Analytical Characterization

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NMR spectra were obtained using a Bruker Avance Neo 400 MHz NMR spectrometer (Billerica, MA, USA). Mass spectra were recorded on a Bruker micro TOF mass spectrometer (ESI-TOF-MS) (Billerica, MA, USA). Optical rotation was obtained using Jasco p-2000 digital polarimeter (Easton, MD, USA). The ECD spectra were calculated by JASCO J-810. UV spectra were measured using a Milton Roy Spectronic 3000 Array spectrophotometer (Rochester, Monroe, NY, USA). IR spectra were determined with a PerkinElmer FT-IR 1760X spectrophotometer (Boston, MA, USA). Semi-preparative HPLC was performed using the Shimadzu HPLC (Kyoto, Japan).

Rivai B., H.a.s.r.i.a.d.i., Dasuni Wasana P.W., Chansriniyom C., Towiwat P., Punpreuk Y., Likhitwitayawuid K., Rojsitthisak P, & Sritularak B. (2023). Potential role of a novel biphenanthrene derivative isolated from Aerides falcata in central nervous system diseases. RSC Advances, 13(16), 10757-10767.

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Characterization of Organic Compounds via NMR

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NMR spectra was recorded on a Bruker AVANCE NEO 400 MHz NMR spectrometer (400 MHz for ¹H-NMR) at 298 K. Chemical shifts were reported in δ (ppm) with the residual deuterated solvent peak as reference and all NMR spectra were performed in deuterated chloroform (modified according to use of deuterium solvent).

Wang Z., Zhao Y, & Wei Y. (2022). Syntheses and properties of tri- and multi-block copolymers consisting of polybutadiene and polylactide segments. RSC Advances, 12(46), 29777-29784.

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Characterization of Organic Compounds

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UV spectra were measured on a Milton Roy Spectronic 300 Array spectrophotometer (Rochester, Monroe, NY, USA). IR spectra were recorded on a Perkin-Elmer FT-IR 1760X spectrophotometer (Boston, MA, USA). Mass spectra were obtained on a Bruker micro TOF mass spectrometer (ESI-MS) (Billerica, MA, USA). NMR spectra were recorded on a Bruker Avance Neo 400 MHz NMR spectrometer (Billerica, MA, USA). Vacuum liquid column chromatography (VLC) and column chromatography (CC) were performed on silica gel 60 (Merck, Kieselgel 60, 70–320 µm) and silica gel 60 (Merck, Kieselgel 60, 230–400 µm) (Darmstadt, Germany).

Jimoh T.O., Costa B.C., Chansriniyom C., Chaotham C., Chanvorachote P., Rojsitthisak P., Likhitwitayawuid K, & Sritularak B. (2022). Three New Dihydrophenanthrene Derivatives from Cymbidium ensifolium and Their Cytotoxicity against Cancer Cells. Molecules, 27(7), 2222.

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Spectroscopic Characterization of Organic Compounds

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Chemicals were purchased from Fisher Scientifics and Avantor VWR International. ¹H NMR (400 MHz) and ¹³C NMR (100 MHz) spectra obtained from a Bruker Avance Neo 400-MHz NMR spectrometer, and were measured from a solution in CDCl₃ unless otherwise stated. The chemical shift data reported in ¹H NMR are given in units of δ relative to TMS (δ = 0) or CHCl₃ (δ = 7.26 ppm). For ¹³C NMR spectra, the chemical shifts are recorded in ppm relative to CDCl₃ (δ = 77.0 ppm). Low-resolution mass spectra were taken from a Waters Acquity TQD Ultra Performance LC/MS/MS system. High-resolution mass spectra obtained using a LCT Premier time of flight mass spectrometer (Waters Inc.). All solvents distilled over appropriated drying agent such as CaH₂ for DMF, dichloromethane and acetonitrile, or Na/benzophenone for THF and diethyl ether. Flash column chromatography carried out on silica gel (400 mesh) for purification of organic products. All compounds were characterized by ¹H and ¹³C NMR spectroscopies, and either low-resolution mass spectrometry for known compounds or high-resolution mass spectrometry for new molecules.

Mossialos D., Meyer J.M., Budzikiewicz H., Wolff U., Koedam N., Baysse C., Anjaiah V, & Cornelis P. (2000). Quinolobactin, a New Siderophore of Pseudomonas fluorescens ATCC 17400, the Production of Which Is Repressed by the Cognate Pyoverdine. Applied and Environmental Microbiology, 66(2), 487-492.

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Purification and Characterization of Organic Compounds

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Chemicals were purchased from Fisher Scientific, VWR international LLC, and Chem-Impex International, Inc. All solvents were dried over appropriated drying agent such as CaH₂ for DMF, dichloromethane, and acetonitrile, Na/benzophenone for THF and Et₂O followed by distillation. Column chromatography was carried out on silica gel (200 – 400 mesh). ¹H NMR spectra (400 MHz) and ¹³C NMR spectra (100 MHz) were recorded on a Varian Unity plus 400-MHz Spectrometer or a Bruker Avance Neo 400-MHz NMR spectrometer. The chemical shift data for each signal on ¹H NMR are given in units of δ relative to TMS (δ = 0 ppm) or CHCl₃ (δ = 7.26 ppm) and on ¹³C NMR are given in units of δ relative to CDCl₃ (δ = 77.0 ppm). Mass spectra were obtained from an API 2000-triple quadrupole ESI-MS/MS mass spectrometer (Applied Biosystems). High-resolution mass spectra were obtained from a LCT Premier (Waters Corp., Milford MA) time of flight mass spectrometer.

Gunaratna M.J., Hao B., Zhang M., Nakagomi M., Ito A., Iwamoto T, & Hua D.H. (2020). SYNTHESIS OF PROBE MOLECULES, 6-(DIMETHYLAMINO)-2-PHENYLISOINDOLIN-1-ONES, FOR MECHANISTIC STUDIES OF FIREFLY LUCIFERASE INHIBITION. Heterocycles, 103(1), 231-248.

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