Uplsapo 100 objective

The images of GFP-PetC1 localization in Synechocystis 6803 thylakoids were acquired by an Olympus FV1000 confocal microscope (Olympus, Japan) equipped with an UPLSAPO 100× objective (NA: 1.40, oil immersion) with the following parameters: excitation 488 nm (Ar-ion laser); dichroic mirror: DM405/488/559/635V; detection of GFP/chlorophyll fluorescence between 500–530 nm and 690–790 nm, respectively. Fluorescence correlation spectroscopy (FCS) was performed with an inverted Zeiss 780 (Carl-Zeiss Jena, Germany) confocal microscope using a water immersion objective (C-Apochromat 40×/1.2 W Korr), Ar-ion excitation (488 nm, 135 µW at the objective) and with detection by a GaAsP detector in photon counting mode (498–576 nm). The diffusion of PetC1-GFP was detected on the bottommost TM (face alignment) and correlation functions were analysed by Fluctuation Analyser 4G v14.3.14. software [35 (link)] using a 2D model (two-component anomalous diffusion with fluorescent protein-like blinking) of diffusion. The correction for GFP fading was applied in the software before the autocorrelogram was calculated.

Sheep whole blood was purchased from Kohjin Bio Co., Ltd. and used within two weeks after blood collection. Fluorescent images of SDS-PAGE gels were acquired with Typhoon 9400 (GE Healthcare). Fluorescent images of cells were acquired using an Olympus IX81 microscope equipped with UPLSAPO 100× objective (Olympus, Tokyo, Japan).