Luminex 200 analyzer

Manufactured by Bio-Rad

Sourced in United States

The Luminex 200™ analyzer is a multiplex assay platform that utilizes color-coded magnetic microspheres to enable simultaneous measurement of multiple analytes in a single sample. The core function of the instrument is to quantify and identify specific proteins, nucleic acids, or other biomolecules present in the sample.

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Lab products found in correlation

Bio plex manager software, by Bio-Rad (2 mentions) Bio plex pro tgf β assay, by Bio-Rad (1 mentions) Luminex assay kit, by R&D Systems (1 mentions) Bio plex manager software version 6, by Bio-Rad (1 mentions)

Close spelling variants of this product

Luminex Bioplex 200 analyzer Luminex 200 Luminex analyzer

3 protocols using luminex 200 analyzer

Multiplex Fibrosis Biomarker Quantification

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The secreted fibrosis markers were measured in the serum samples of patients by multiplex fluorescent-bead-based technology (Luminex 200, Austin, TX, USA) using two commercial Luminex screening assay kits: a customized Luminex Assay kit from R&D Systems (Lilles, France) for MMP-1, resistin, Collagen IA1, PDGF-BB and TIMP-1 and the Bio-Plex Pro TGF-β assay from Bio–Rad Laboratories (Marnes-la-Coquette, France).
In brief, the samples were diluted before incubation with specific antibody-coated fluorescent beads according to the manufacturer’s recommendations. After washing, 50 beads were analyzed with the Luminex 200™ analyzer and Bio-Plex Manager software version 6 (Bio–Rad Laboratories), and the analyte concentrations of the samples were estimated through the serial dilution of cytokine standards (MMP-1 sensitivity < 3 pg/mL; resistin sensitivity < 20 ng/mL; Col IA1 sensitivity < 100 pg/mL; PDGF-BB sensitivity < 50 pg/mL; TIMP-1 sensitivity < 800 pg/mL; and TGF-β1 sensitivity < 15 pg/mL).

Lefeuvre C., Roux M., Blanchard S., Le Guillou-Guillemette H., Boursier J., Lunel-Fabiani F., Jeannin P., Pivert A, & Ducancelle A. (2022). Analysis of hepatic fibrosis markers in the serum of chronic hepatitis B patients according to basal core promoter/precore mutants. Scientific Reports, 12, 10261.

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Cytokinome Profiling Using Luminex

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The cytokinome was determined in the FF by multiplex fluorescent-bead-based technology (Luminex 200™, Austin, TX, USA), using two commercial Luminex screening assay kits (Bio-Plex Pro™ Human Cytokine 27-plex Assay and Bio-Plex Pro Human Inflammation Panel 1, 37-plex Bio-Rad) from Bio-Rad Laboratories (Marnes-la-Coquette, IDF, France), allowing the measurement of 57 cytokines and other inflammation-associated factors (Matrix MetalloProteinase-3 (MMP-3), Chitinase-3 like 1 protein). Briefly, the FF samples were diluted two-fold before incubation with specific antibody-coated fluorescent beads according to the manufacturer’s recommendations. After washing, 50 beads were analyzed with the Luminex 200™ analyzer and Bio-Plex Manager software version 6 (Bio-Rad Laboratories, Hercules, CA, USA, 2010), and the cytokine concentrations of the samples were estimated through the serial dilution of cytokine standards.

Bouet P.E., Chao de la Barca J.M., Boucret L., Descamps P., Legendre G., Hachem H.E., Blanchard S., Jeannin P., Reynier P, & May-Panloup P. (2020). Elevated Levels of Monocyte Chemotactic Protein-1 in the Follicular Fluid Reveals Different Populations among Women with Severe Endometriosis. Journal of Clinical Medicine, 9(5), 1306.

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Macrophage Phenotype on Scaffold Membranes

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Macrophages were seeded on different membranous scaffolds (PCL, PCL-epECM/H, PCL-epECM/H-IL-4) at a density of 5 × 10⁵ cells/well with 1640 medium supplemented with 5% heat-inactivated FBS in 6-well plates. The macrophages seeded on blank plates were set as negative control (labeled as TCP). The macrophages seeded on blank plates with the addition of 10 ng/mL IL-4 into the medium were set as the positive control (labeled as IL-4) (n = 3). The supernatants were collected at 1 day and 3 days, centrifuged at 250 g for 8 min and immediately stored at −80 °C. Then the magnetic beads (Luminex) multiplexed cytokine assays was performed, according to the manufacturer's recommendations. Magnetic beads with 7 distinct spectral signatures (regions) were coupled to cytokine-specific capture antibodies without cross-reactivity. Data were collected on Luminex 200 analyzer and analyzed using Bioplex Manager software (Bio-Rad). 4P regression algorithms was used to determine cytokine concentrations, values below the lower limit of quantification were set to zero.

Liu S., Yao L., Wang Y., Li Y., Jia Y., Yang Y., Li N., Hu Y., Kong D., Dong X., Wang K, & Zhu M. (2022). Immunomodulatory hybrid micro-nanofiber scaffolds enhance vascular regeneration. Bioactive Materials, 21, 464-482.

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