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Sumatriptan

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Sumatriptan is a synthetic drug that acts as a serotonin receptor agonist. It is commonly used as a reference standard in the analysis and characterization of pharmaceutical products.

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Lab products found in correlation

Picrotoxin, by Merck Group (2 mentions) U73122, by Bio-Techne (2 mentions) Am251, by Bio-Techne (2 mentions) Gr55562, by Bio-Techne (2 mentions) Phenylephrine, by Bio-Techne (2 mentions) Jnj16259685, by Bio-Techne (2 mentions) D 2 amino 5 phosphonopentanoic acid d ap5, by Bio-Techne (2 mentions) Tamoxetine, by Bio-Techne (2 mentions) Gbr12783, by Bio-Techne (2 mentions) Way 100635, by Abcam (2 mentions) Citalopram, by Bio-Techne (2 mentions) Sulpiride, by Bio-Techne (2 mentions) Quinpirole, by Bio-Techne (2 mentions) L 745870, by Bio-Techne (2 mentions) Gabazine, by Abcam (2 mentions) Kynurenic acid, by Abcam (2 mentions) Compound 48 80, by Merck Group (1 mentions) Peg400, by Merck Group (1 mentions) Phenytoin, by Merck Group (1 mentions) Naproxen, by Bio-Techne (1 mentions)

9 protocols using sumatriptan

1

Treating Post-Traumatic Headache in Rats

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Sumatriptan (Tocris, USA) was freshly dissolved in 0.9% saline and administered intra-peritoneal (i.p.) at a dose of 1 mg/kg in a volume of 1 ml/kg. Drug dose and times of administration were based on the pharmacokinetics of the drugs, studies demonstrating their efficacy in animal and human models of trigeminal pain (16 (link),23 (link)), and our previous data on PTH-like behaviors following mCHI in males (4 (link)). Anti-CGRP mAb and its corresponding isotype IgG were provided by TEVA Pharmaceuticals and formulated in phosphate-buffered saline (PBS). mAb and IgG were injected i.p. at a dose of 30 mg/kg, immediately after the head injury and every 6 days subsequently up to day 30. This dosing regimen has been shown previously to alleviate PTH-like pain behavior (i.e. cephalic allodynia) following mCHI in male rats (4 (link)) and pain behaviors in other chronic migraine models (24 (link)).
Bree D., Mackenzie K., Stratton J, & Levy D. (2020). Enhanced post-traumatic headache-like behaviors and diminished contribution of peripheral CGRP in female rats following a mild closed head injury. Cephalalgia : an international journal of headache, 40(7), 748-760.
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2

Intravascular and Supra-dural Injection Protocols

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2AT and C391 were made as previously described (21 (link)). Compound 48/80 was purchased from Sigma-Aldrich. Sumatriptan was purchased from Tocris. Wheat germ agglutinin conjugated to Alexa-Fluor 555 was purchased from Life Technologies and dissolved in synthetic interstitial fluid (SIF) (NaCl 107.8 mM, KCl 3.5 mM, CaCl2.2H2O 1.53 mM, MgSO4.7H2O 0.7 mM, NaH2PO4.2H2O 1.67 mM, NaHCO3 26.2 mM, C6H11NaO7 9.65 mM, Sucrose 7.6 mM, Glucose 5.55 mM, pH 7.4, 310 mOsm). Vehicle controls were SIF with 1% DMSO for supra-dural injections or saline (0.9%) for intravenous injections.
Hassler S.N., Ahmad F.B., Burgos-Vega C.C., Boitano S., Vagner J., Price T.J, & Dussor G. (2018). Protease activated receptor 2 (PAR2) activation causes migraine-like pain behaviors in mice. Cephalalgia : an international journal of headache, 39(1), 111-122.
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3

Multimodal Analgesic Regimen for Neuropathic Pain

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Naproxen (Tocris, 10 mg/kg), codeine (St. Martins Pharmacy, 7 or 30 mg/kg), sumatriptan (Tocris, 10 mg/kg), and gabapentin (Sigma, 30 mg/kg) were dissolved in physiological saline (Braun) and administered via a single i.p. injection. Phenytoin (Sigma, 15 mg/kg) and aprepitant (MedChemExpress, 20 mg/kg) were dissolved in PEG-400 (Sigma) and delivered via i.p. injections. Drug injection schedules are detailed in Figure 1A: Naproxen, sumatriptan, and aprepitant were administered once on the last testing day. Codeine was administered at the last testing day twice (1 h before testing and when testing was concluded–before the night cycle). Phenytoin was administered once daily over a 15 day period, starting with the first injection of oxaliplatin. gabapentin was injected once per day over a period of 5 consecutive days starting 2 days after CCI-surgery.
Draxler P., Moen A., Galek K., Boghos A., Ramazanova D, & Sandkühler J. (2021). Spontaneous, Voluntary, and Affective Behaviours in Rat Models of Pathological Pain. Frontiers in Pain Research, 2, 672711.
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4

Pharmacological Manipulations in Slice Experiments

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For in vitro experiments, drugs were first dissolved in vehicle to make 1,000- or 10,000-fold concentrated stock solutions. The drugs were then applied to the slices by diluting the stock solution in the perfusion reservoir. DNQX, D-(−)-2-amino-5-phosphonopentanoic acid (D-AP5), GR55562, HEAT, JNJ16259685, MTEP, AM 251, THL, phenylephrine, sumatriptan, and U73122 were purchased from Tocris. Picrotoxin was purchased from Sigma. AAV1.Syn.Flex.GCaMP6f was purchased from the University of Pennsylvania Vector Core. 5-(4-Chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-3-[(E)-piperi-dinoiminomethyl]-1H-pyrazole (PIMSR1) was provided by Dr. Herbert H. Seltz-man at Research Triangle Institute (NIDA, Raleigh, NC; RTI International). For FSCV experiments, cocaine (Sigma-Aldrich) was dissolved in saline, rimonabant (SR141716A; RTI), and THL (Sigma-Aldrich) were freshly suspended in a 1:1:18 ratio of ethanol, emulphor (Alkamuls EL-620; Rhodia), and saline (0.9%).
Wang H., Treadway T., Covey D.P., Cheer J.F, & Lupica C.R. (2015). Cocaine-Induced Endocannabinoid Mobilization in the Ventral Tegmental Area. Cell reports, 12(12), 1997-2008.
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5

Pharmacological Manipulations in Slice Experiments

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For in vitro experiments, drugs were first dissolved in vehicle to make 1,000- or 10,000-fold concentrated stock solutions. The drugs were then applied to the slices by diluting the stock solution in the perfusion reservoir. DNQX, D-(−)-2-amino-5-phosphonopentanoic acid (D-AP5), GR55562, HEAT, JNJ16259685, MTEP, AM 251, THL, phenylephrine, sumatriptan, and U73122 were purchased from Tocris. Picrotoxin was purchased from Sigma. AAV1.Syn.Flex.GCaMP6f was purchased from the University of Pennsylvania Vector Core. 5-(4-Chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-3-[(E)-piperi-dinoiminomethyl]-1H-pyrazole (PIMSR1) was provided by Dr. Herbert H. Seltz-man at Research Triangle Institute (NIDA, Raleigh, NC; RTI International). For FSCV experiments, cocaine (Sigma-Aldrich) was dissolved in saline, rimonabant (SR141716A; RTI), and THL (Sigma-Aldrich) were freshly suspended in a 1:1:18 ratio of ethanol, emulphor (Alkamuls EL-620; Rhodia), and saline (0.9%).
Wang H., Treadway T., Covey D.P., Cheer J.F, & Lupica C.R. (2015). Cocaine-Induced Endocannabinoid Mobilization in the Ventral Tegmental Area. Cell reports, 12(12), 1997-2008.
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6

Pharmacological Agents and Administration

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Ketamine/xylazine was purchased from Western Medical Supply (Arcadia, CA). 17-β-estradiol, ICI 182,780 and sumatriptan were purchased from Tocris (Ellisville, MO). 17-β-estradiol and ICI 182,780 were dissolved in castor oil to appropriate concentrations the day of experiments. sumatriptan was dissolved in saline. Castor oil and sterile saline (0.9%) served as appropriate controls. All drugs were administered via intraperitoneal (i.p.) injections.
Sandweiss A.J., Cottier K.E., McIntosh M.I., Dussor G., Davis T.P., Vanderah T.W, & Largent-Milnes T.M. (2017). 17-β-Estradiol induces spreading depression and pain behavior in alert female rats. Oncotarget, 8(69), 114109-114122.
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7

Pharmacological Reagents Inventory

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Cocaine HCl was obtained from the National Institute on Drug Abuse. NBQX, CPP, sulpiride, quinpirole, sumatriptan, GBR12783, tamoxetine, citalopram, 5-HT, and L-745870 were purchased from Tocris. Gabazine, kynurenic acid (sodium salt), and WAY 100635 were obtained from Abcam. All other chemicals were from Sigma.
Matsui A, & Alvarez V.A. (2018). Cocaine Inhibition of Synaptic Transmission in the Ventral Pallidum Is Pathway-Specific and Mediated by Serotonin. Cell reports, 23(13), 3852-3863.
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8

Pharmacological Reagents Inventory

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Cocaine HCl was obtained from the National Institute on Drug Abuse. NBQX, CPP, sulpiride, quinpirole, sumatriptan, GBR12783, tamoxetine, citalopram, 5-HT, and L-745870 were purchased from Tocris. Gabazine, kynurenic acid (sodium salt), and WAY 100635 were obtained from Abcam. All other chemicals were from Sigma.
Matsui A, & Alvarez V.A. (2018). Cocaine Inhibition of Synaptic Transmission in the Ventral Pallidum Is Pathway-Specific and Mediated by Serotonin. Cell reports, 23(13), 3852-3863.
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9

Serotonin Receptor 1B Antagonist Effects

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Cell viability and proliferation were detected by MTS Assay (Promega, Madison, WI) after treatment with 5-HTR1B antagonist SB216641 (Tocris Inc.) [11, 14, 17] . Briefly, huLM cells were seeded into 96-well plates (2 Â 10 3 cells/well) and treated with 2, 4, 6, 8 and 10 mM doses of SB216641 for 72 h. huLM cells were also pretreated with 5 mM NAC, an antioxidant compound, for 24 h or 5 mM Sumatriptan (Tocris Inc.), a serotonin 5-HT1B agonist, for 6 h prior to SB216641 treatments and cell viability was detected by of MTS. Viable cells were quantified by absorbance levels at 490 nm wavelength using Spectrophotometry. All experiments were performed in triplicate and the results were given as mean AE standard deviation (SD).
Gurbuz N., Asoglu M.R., Ashour A.A., Salama S., Kilic G.S, & Ozpolat B. (2016). A selective serotonin 5-HT1B receptor inhibition suppresses cells proliferation and induces apoptosis in human uterine leiomyoma cells. European journal of obstetrics, gynecology, and reproductive biology, 206.
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