Selexion

The SelexIon (Sciex, Framingham, MA, USA, SelexIon-technology">https://sciex.com/technology/SelexIon-technology) was utilized for all DMS analyses. The “low” setting was selected for the DMS temperature, which equates to 150°C. Ethyl acetate was the DMS modifier (MOD) and its composition was set to “low”, which corresponded to 1.5% v/v. The DMS offset was −3.0V and the DMS resolution enhancement (DR) was turned off. The separation voltage (SV) was 3500 V. The compensation voltage (COV) for each acylcarnitine was tuned prior to sample analysis. All DMS data were acquired with Analyst 1.6.2 (Sciex, Framingham, MA, USA).

The DMS instrument utilized was a SelexIon (Sciex, Framingham, MA, USA). The DMS conditions were as follows: DMS temperature (DT), low (150°C); modifier (MOD), ethyl acetate; modifier composition (MDC), low (1.5% v/v); DMS offset (DMO), −3.0 V; DMS resolution enhancement (DR), off. Optimal signal for acetylcarnitine was determined by varying the separation voltage (SV) and the compensation voltage (COV). With this system, we found that COV values for acetylcarnitine should be optimized daily. As a result, the COV values used for our analyses ranged from −46V to −37V. This change did not make any significant change to intensities. DMS data were acquired with Analyst 1.6.2.