Plasma ET-1 was measured using Human ET-1 Quanti-Glo ELISA (R&D Systems, Minneapolis, MN). Insulin was measured with the Human Insulin ELISA (Crystal Chem, Elk Grove Village, IL).
Human insulin elisa
Manufactured by Crystal Chem
The Human Insulin ELISA is a quantitative test kit used to measure the concentration of human insulin in biological samples, such as serum or plasma. It employs the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the presence of human insulin. The kit provides the necessary reagents and materials to perform the assay.
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Lab products found in correlation
2 protocols using human insulin elisa
1
Plasma ET-1 and Insulin Measurement
2
Insulin Secretion Assay in EndoCβH2-Cre Cells
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Static insulin secretion was assessed as previously described (22 (link)) in EndoCβH2-Cre cells, which involved a 1-h incubation at 37°C in 2.5 mmol/L glucose secretion assay buffer, and then with freshly prepared 2.5 or 16.7 mmol/L glucose secretion assay buffer for another hour. The outcome was presented as the secreted insulin (Lumit Insulin Immunoassay, Promega, and human insulin ELISA, Crystal Chem) relative to the total insulin content. The secreted insulin data were normalized to basal secretion of the Sham at 2.5 mmol/L glucose. Insulin content was presented as the concentration of insulin normalized to total DNA content (Quant-iT PicoGreen; Invitrogen) in each condition (ng/ng DNA) relative to the Sham. At least three independently generated sets of samples were analyzed under each condition.
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